The global carbon metabolism regulator Crc is a component of a signal transduction pathway required for biofilm development by Pseudomonas aeruginosa

Journal of Bacteriology

Volumn 182, Issue 2, 2000, Pages 425-431

  O'Toole, George A ^a,b   Gibbs, Karine A ^b   Hager, Paul W ^c   Phibbs Jr , Paul V ^c   Kolter, Roberto ^b  

^a DARTMOUTH MEDICAL SCHOOL   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c EAST CAROLINA UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ARTICLE; BACTERIAL GROWTH; BACTERIAL METABOLISM; BACTERIUM COLONY; BACTERIUM CULTURE; BIOFILM; CARBON DIOXIDE FIXATION; MICROSCOPY; NONHUMAN; PRIORITY JOURNAL; PSEUDOMONAS AERUGINOSA; SIGNAL TRANSDUCTION;

BACTERIAL PROTEINS; BIOFILMS; CARBON; MUTATION; PHENOTYPE; PSEUDOMONAS AERUGINOSA; REPRESSOR PROTEINS; SIGNAL TRANSDUCTION;

EID: 0033989302     PISSN: 00219193     EISSN: None     Source Type: Journal    
DOI: 10.1128/JB.182.2.425-431.2000     Document Type: Article

References (37)

1. Ausubel, F. A., R. Brent, R. E. Kingston, D. D. Moore, J. G. Seidman, J. A. Smith, and K. Struhl. 1990. Current protocols in molecular biology. Wiley Interscience, New York, N.Y.
2. Barzilay, G., and I. D. Hickson. 1995. Structure and function of apurinic/ apyrimidimic endonucleases. Bioessays 178:713-719.
3. Bloemberg, G. V., G. A. O'Toole, B. J. J. Lugtenberg, and R. Kolter. 1997. Green fluorescent protein as a marker for Pseudomonas spp. Appl. Environ. Microbiol. 63:4543-4551.
4. Bright, B. D., S. K. Arora, P. V. Phibbs, and A. Prince. 1995. Involvement of the crc locus in the regulation of the expression of Pseudomonas aeruginosa virulence factors. Pediatr. Pulm. 128:244-245.
5. Collier, D. N., C. Spence, M. J. Cox, and P. V. Phibbs. Isolation and characterization of Pseudomonas aeruginosa pseudorevertants containing suppressors of the catabolite repression control defective crc-10 allele. Submitted for publication.
6. Costerton, J. W., Z. Lewandowski, D. E. Caldwell, D. R Korber, and H. M. Lappin-Scott. 1995. Microbial biofilms. Annu. Rev. Microbiol. 49:711-745.
7. Davies, D. G., A. M. Chakabarty, and G. G. Geesey. 1993. Exopolysaccharide production in biofilms: substratum activation of alginate gene expression by Pseudomonas aeruginosa. Appl. Environ. Microbiol. 59:1181-1186.
8. Davies, D. G., and G. G. Geesey. 1995. Regulation of the alginate biosynthesis gene algC in Pseudomonas aeruginosa during biofilm development in continuous culture. Appl. Environ. Microbiol. 61:860-867.
9. Doetsch, P. W., and R. P. Cunningham. 1990. The enzymology of apurinic/ apyrimidimic endonucleases. Mutat. Res. 236:173-201.
10. Evans, L. R., and A. Linker. 1973. Production and characterization of the slime polysaccharide of Pseudomonas aeruginosa. J. Bacteriol. 116:915-924.
11. Fletcher, M. 1977. The effects of culture concentration and age, time, and temperature on bacterial attachment to polystyrene. Can. J. Microbiol. 23: 1-6.
12. Grossman, A. 1995. Genetic networks controlling the initiation of sporulation and the development of genetic competence in Bacillus subtilis. Annu. Rev. Genet. 29:477-508.
13. Hoyle, B. D., and W. J. Costerton. 1991. Bacterial resistance to antibiotics: the role of biofilms. Prog. Drug Res. 37:91-105.
14. Hylemon, P. B., and P. V. Phibbs, Jr. 1972. Independent regulation of hexose catabolizing enzymes and glucose transport activity in Pseudomonas aeruginosa. Biochem. Biophys. Res. Commun. 48:1041-1048.
15. Jensen, E. C., H. S. Schrader, B. Rieland, T. L. Thompson, K. W. Lee, K. W. Nickerson, and T. A. Kokjohn. 1998. Prevalence of broad-host-range lytic bacteriophages of Sphaerotilus natans, Escherichia coli, and Pseudomonas aeruginosa. Appl. Environ. Microbiol. 64:575-580.
16. Koga, T., K. Ishimoto, and S. Lory. 1993. Genetic and functional characterization of the gene cluster specifying expression of Pseudomonas aeruginosa pili. Infect. Immun. 61:1371-1377.
17. Lowry, O. H., N. J. Rosebrough, A. L. Farr, and R. J. Randall. 1951. Protein measurement with the Folin phenol reagent. J. Biol. Chem. 193:265-275.
18. Lynch, W. H., and M. Franklin. 1978. Effect of temperature on diauxic growth with glucose and organic acids in Pseudomonas fluorescens. Arch. Microbiol. 118:131-140.
19. MacGregor, C. H., S. K. Arora, P. W. Hager, M. B. Dail, and P. V. Phibbs, Jr. 1996. The nucleotide sequence of the Pseudomonas aeruginosa pyrE-crc-rph region and the purification of the crc gene product. J. Bacteriol. 178: 5627-5635.
20. MacGregor, C. H., J. A. Wolff, S. K. Arora, and P. V. Phibbs, Jr. 1991. Cloning of the catabolite repression control (crc) gene from Pseudomonas aeruginosa, expression of the gene in Escherichia coli, and identification of the gene product in Pseudomonas aeruginosa. J. Bacteriol. 173:7204-7212.
21. Mattick, J. S., C. B. Whitchurch, and R. A. Alm. 1996. The molecular genetics of type-4 fimbriae in Pseudomonas aeruginosa - a review. Gene 179:147-155.
22. Miller, J. H. 1992. A short course in bacterial genetics. Cold Spring Harbor Laboratory Press, Cold Spring Harbor, N.Y.
23. Nunn, D., S. Bergman, and S. Lory. 1990. Products of three accessory genes, pilB, pilC, and pilD, are required for biogenesis of Pseudomonas aeruginosa pili. J. Bacteriol. 172:2911-2919.
24. O'Toole, G. A., and R. Kolter. 1998. Flagellar and twitching motility are necessary for Pseudomonas aeruginosa biofilm development. Mol. Microbiol. 30:295-304.
25. O'Toole, G. A., and R. Kolter. 1998. The initiation of biofilm formation in Pseudomonas aeruginosa WCS365 proceeds via multiple, convergent signaling pathways: a genetic analysis. Mol. Microbiol. 28:449-461.
26. O'Toole, G. A., and R. Kolter. 1999. Unpublished data.
27. O'Toole, G. A., L. A. Pratt, P. I. Watnick, D. K. Newman, V. B. Weaver, and R. Kolter. 1999. Genetic approaches to the study of biofilms. Methods Enzymol. 310:91-109.
28. Pardee, A. B., F. Jacob, and J. Monod. 1959. The genetic control and cytoplasmic expression of "inducibility" in the synthesis of β-galactosidase in E. coli. J. Mol. Biol. 1:165-178.
29. Phillips, A. T., and L. M. Mulfinger. 1981. Cyclic adenosine 3′,5′-monophosphate levels in Pseudomonas putida and Pseudomonas aeruginosa during induction and carbon catabolite repression in histidase synthesis. J. Bacteriol. 145:1285-1292.
30. Pratt, L. A., and R. Kolter. 1998. Genetic analysis of Escherichia coli biofilm formation: defining the roles of flagella, motility, chemotaxis and type I pili. Mol. Microbiol. 30:285-294.
31. Rahme, L. G., E. J. Stevens, S. F. Wolfort, J. Shao, R. G. Tompkins, and F. M. Ausubel. 1995. Common virulence factors for bacterial pathogenicity in plants and animals. Science 268:1899-1902.
32. Schweizer, H. P. 1991. Escherichia-Pseudomonas shuttle vectors derived from pUC18/19. Gene 103:109-112.
33. Shapiro, J. A., and M. Dworkin (ed.). 1997. Bacteria as multicellular organisms. Oxford University Press, Inc., New York, N.Y.
34. Siegel, L. S., P. B. Hylemon, and P. V. Phibbs, Jr. 1977. Cyclic adenosine 3′,5′-monophosphate levels and activities of adenylate cyclase and cyclic adenosine 3′,5′-monophosphate phosphodiesterase in Pseudomonas and Bacteroides. J. Bacteriol. 129:87-96.
35. Tiwari, N. P., and J. R. R. Campbell. 1969. Enzymatic control of the metabolic activity of Pseudomonas aeruginosa grown in glucose or succinate media. Biochim. Biophys. Acta 192:395-401.
36. Wimpenny, J. W. T., and R. Colasanti. 1997. A unifying hypothesis for the structure of microbial biofilms based on cellular automation models. FEMS Microbiol. Ecology 22:1-16.
37. Wolff, J. A., C. H. MacGregor, R. C. Eisenberg, and P. V. Phibbs, Jr. 1991. Isolation and characterization of catabolite repression control mutants of Pseudomonas aeruginosa PAO. J. Bacteriol. 173:4700-4706.