Metabolic engineering for the production of polyunsaturated fatty acids by oleaginous fungus Mortierella alpina 1S-4

Journal of Bioscience and Bioengineering

Volumn 116, Issue 4, 2013, Pages 417-422

  Sakuradani, Eiji ^a   Ando, Akinori ^a   Shimizu, Sakayu ^b   Ogawa, Jun ^a  

^a KYOTO UNIVERSITY   (Japan)

^b Ukyo ku   (Japan)

Author keywords

Arachidonic acid (AA); Eicosapentaenoic acid (EPA); Fatty acid desaturase; Molecular breeding; Mortierella alpina; Polyunsaturated fatty acid

Indexed keywords

ARACHIDONIC ACIDS; DESATURASE; EICOSAPENTAENOIC ACID; MOLECULAR BREEDING; MORTIERELLA ALPINA;

BIOCHEMISTRY; BIOSYNTHESIS; ENZYMES; FATTY ACIDS; GENES; SEED;

POLYUNSATURATED FATTY ACIDS;

ARACHIDONIC ACID; FISH OIL; POLYUNSATURATED FATTY ACID; TRIACYLGLYCEROL;

BIOSYNTHESIS; BREEDING; FUNGAL GENE; FUNGAL STRAIN; GENETIC MANIPULATION; ISOLATION PROCEDURE; METABOLIC ENGINEERING; MORTIERELLA ALPINA; MUTANT; NONHUMAN; NUTRITIONAL REQUIREMENT; PLANT SEED; PRODUCTIVITY; REVIEW;

FUNGI; MORTIERELLA ALPINA;

ARACHIDONIC ACID (AA); EICOSAPENTAENOIC ACID (EPA); FATTY ACID DESATURASE; MOLECULAR BREEDING; MORTIERELLA ALPINA; POLYUNSATURATED FATTY ACID;

ARACHIDONIC ACID; BIOSYNTHETIC PATHWAYS; EICOSAPENTAENOIC ACID; FATTY ACID DESATURASES; FATTY ACIDS, UNSATURATED; METABOLIC ENGINEERING; MORTIERELLA; MUTATION; TRANSFORMATION, GENETIC; TRIGLYCERIDES;

EID: 84883284901     PISSN: 13891723     EISSN: 13474421     Source Type: Journal    
DOI: 10.1016/j.jbiosc.2013.04.008     Document Type: Review

References (34)

1. Carlson S.E., Werkman S.H., Peeples J.M., Cooke R.J., Tolley E.A. Arachidonic acid status correlates with first year growth in preterm infants. Proc. Natl. Acad. Sci. USA 1993, 90:1073-1077.
2. Gill I., Valivety R. Polyunsaturated fatty acids, part 1: occurrence, biological activities and application. Trends Biotechnol. 1997, 15:401-409.
3. Shimokawa H. Beneficial effects of eicosapentaenoic acid on endothelial vasodilator functions in animals and humans. World Rev. Nutr. Diet. 2001, 88:100-108.
4. Mori T.A., Beilin L.J., Burke V., Morris J., Ritchie J. Interactions between dietary fat, fish, and fish oils and their effects on platelet function in men at risk of cardiovascular disease. Arterioscler. Thromb. Vasc. Biol. 1997, 17:279-286.
5. Qi B., Fraser T., Mugford S., Dobson G., Sayanova O., Butler J., Napier J.A., Stobart A.K., Lazarus C.M. Production of very long chain polyunsaturated ω3 and ω6 fatty acids in plants. Nat. Biotechnol. 2004, 22:739-745.
6. Ratledge C. Microbial lipids: commercial realities or academic curiosities. Industrial applications of single cell oils 1992, 1-15. AOCS Press, IL. D.J. Kyle, C. Ratledge (Eds.).
7. Ratledge C. Single cell oils-have they a biotechnological future?. Trends Biotechnol. 1993, 11:278-284.
8. Shimizu S., Kawashima H., Shinmen Y., Akimoto K., Yamada H. Production of eicosapentaenoic acid by Mortierella fungi. J.Am. Oil Chem. Soc. 1988, 65:1455-1459.
9. Shinmen Y., Shimizu S., Yamada H. Production of arachidonic acid by Mortierella fungi: selection of a potent producer and optimization of culture conditions for large-scale production. Appl. Microbiol. Biotechnol. 1989, 31:11-16.
10. Yamada H., Shimizu S., Shinmen Y. Production of arachidonic acid by Mortierell elongata 1S-5. Agric. Biol. Chem. 1987, 51:785-790.
11. Jareonkitmongkol S., Shimizu S., Yamada H. Fatty acid desaturation defective mutants of an arachidonic acid-producing fungus, Mortierella alpina 1S-4. J.Gen. Microbiol. 1992, 138:997-1002.
12. Certik M., Sakuradani E., Shimizu S. Desaturase-defective fungal mutants: useful tools for the regulation and overproduction of polyunsaturated fatty acids. Trends Biotechnol. 1998, 16:500-505.
13. Jareonkitmongkol S., Sakuradani E., Shimizu S. Isolation and characterization of a δ9-desaturation-defective mutant of an arachidonic acid-producing fungus, Mortierella alpina 1S-4. J.Am. Oil Chem. Soc. 2002, 79:1021-1026.
14. Sakuradani E., Kamada N., Hirano Y., Nishihara M., Kawashima H., Akimoto K., Higashiyama K., Ogawa J., Shimizu S. Production of 5,8,11-cis-eicosatrienoic acid by a δ5 and δ6 desaturation activity-enhanced mutant derived from a δ12 desaturation activity-defective mutant of Mortierella alpina 1S-4. Appl. Microbiol. Biotechnol. 2002, 60:281-287.
15. Kamada N., Kawashima H., Sakuradani E., Akimoto K., Ogawa J., Shimizu S. Production of 8,11-cis-eicosadienoic acid by a δ5 and δ12 desaturase-defective mutant derived from the arachidonic acid producing fungus Mortierella alpina 1S-4. J.Am. Oil Chem. Soc. 1999, 76:1269-1274.
16. Jareonkitmongkol S., Shimizu S., Yamada H. Occurrence of two nonmethylene-interrupted fatty acids in a δ6-desaturase-defective mutant of the fungus Mortierella alpina 1S-4. Biochim. Biophys. Acta 1993, 1167:137-141.
17. Jareonkitmongkol S., Sakuradani E., Shimizu S. Anovel δ5-desaturase-defective mutant of Mortierella alpina 1S-4 and its dihomo-γ-linolenic acid productivity. Appl. Environ. Microbiol. 1993, 59:4300-4304.
18. Sakuradani E., Hirano Y., Kamada N., Nojiri M., Ogawa J., Shimizu S. Improvement of arachidonic acid production by mutants with lowered ω3-desaturation activity derived from Mortierella alpina 1S-4. Appl. Microbiol. Biotechnol. 2004, 66:243-248.
19. 9-Fatty acid desaturase from arachidonic acid-producing fungus. Unique gene sequence and its heterologous expression in a fungus, Aspergillus. Eur. J. Biochem. 1999, 260:208-216.
20. Abe T., Sakuradani E., Asano T., Kanamaru H., Shimizu S. Functional characterization of δ9 and ω9 desaturase genes in Mortierella alpina 1S-4 and its derivative mutants. Appl. Microbiol. Biotechnol. 2006, 70:711-719.
21. Sakuradani E., Shimizu S. Gene cloning and functional analysis of a second δ6-fatty acid desaturase from an arachidonic acid-producing Mortierella fungus. Biosci. Biotechnol. Biochem. 2003, 67:704-711.
22. Sakuradani E., Abe T., Iguchi K., Shimizu S. Anovel fungal ω3-desaturase with wide substrate specificity from arachidonic acid-producing Mortierella alpina 1S-4. Appl. Microbiol. Biotechnol. 2005, 66:648-654.
23. Sakuradani E., Nojiri M., Suzuki H., Shimizu S. Identification of a novel fatty acid elongase with a wide substrate specificity from arachidonic acid-producing fungus Mortierella alpina 1S-4. Appl. Microbiol. Biotechnol. 2009, 84:709-716.
24. Parker-Barnes J.M., Das T., Bobik E., Leonard A.E., Thurmond J.M., Chaung L.T., Huang Y.S., Mukerji P. Identification and characterization of an enzyme involved in the elongation of n-6 and n-3 polyunsaturated fatty acids. Proc. Natl. Acad. Sci. USA 2000, 97:8284-8289.
25. Sakuradani E., Murata S., Kanamaru H., Shimizu S. Functional analysis of a fatty acid elongase from arachidonic acid-producing Mortierella alpina 1S-4. Appl. Microbiol. Biotechnol. 2008, 81:497-503.
26. Takeno S., Sakuradani E., Murata S., Inohara-Ochiai M., Kawashima H., Ashikari T., Shimizu S. Cloning and sequencing of the ura3 and ura5genes, and isolation and characterization of uracil auxotrophs of the fungus Mortierella alpina 1S-4. Biosci. Biotechnol. Biochem. 2004, 68:277-285.
27. Takeno S., Sakuradani E., Murata S., Inohara-Ochiai M., Kawashima H., Ashikari T., Shimizu S. Establishment of an overall transformation system for an oil-producing filamentous fungus, Mortierella alpina 1S-4. Appl. Microbiol. Biotechnol. 2004, 65:419-425.
28. Ando A., Sumida Y., Negoro H., Suroto D.A., Ogawa J., Sakuradani E., Shimizu S. Establishment of Agrobacterium tumefaciens-mediated transformation of an oleaginous fungus, Mortierella alpina 1S-4, and its application for eicosapentaenoic acid producer breeding. Appl. Environ. Microbiol. 2009, 75:5529-5535.
29. Takeno S., Sakuradani E., Tomi A., Inohara-Ochiai M., Kawashima H., Shimizu S. Transformation of oil-producing fungus, Mortierella alpina 1S-4, using Zeocin, and application to arachidonic acid production. J.Biosci. Bioeng. 2005, 100:617-622.
30. Ando A., Sakuradani E., Horinaka K., Ogawa J., Shimizu S. Transformation of an oleaginous zygomycete Mortierella alpina 1S-4 with the carboxin resistance gene conferred by mutation of the iron-sulfur subunit of succinate dehydrogenase. Curr. Genet. 2009, 55:349-356.
31. 560 subunit of succinate dehydrogenase complex (Complex II). Mol. Genet. Genomics 2004, 272:328-335.
32. Wynn J.P., Ratledge C. Evidence that the rate-limiting step for the biosynthesis of arachidonic acid in Mortierella alpina is at the level of the 18:3 to 20:3 elongase. Microbiology 2000, 146:2325-2331.
33. Takeno S., Sakuradani E., Tomi A., Inohara-Ochiai M., Kawashima H., Ashikari T., Shimizu S. Improvement of the fatty acid composition of an oil-producing filamentous fungus, Mortierella alpina 1S-4, through RNA interference with δ12-desaturase gene expression. Appl. Environ. Microbiol. 2005, 71:5124-5128.
34. Takeno S., Sakuradani E., Murata S., Inohara-Ochiai M., Kawashima H., Ashikari T., Shimizu S. Molecular evidence that the rate-limiting step for the biosynthesis of arachidonic acid in Mortierella alpina is at the level of an elongase. Lipids 2005, 40:25-30.