Functional analysis of a fatty acid elongase from arachidonic acid-producing Mortierella alpina 1S-4

Applied Microbiology and Biotechnology

Volumn 81, Issue 3, 2008, Pages 497-503

  Sakuradani, Eiji ^a   Murata, Shoichi ^a   Kanamaru, Hiroyuki ^a   Shimizu, Sakayu ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

Fatty acid elongase; Functional analysis; Gene expression; Mortierella; RNA interference

Indexed keywords

BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; CARBON; FUNCTIONAL ANALYSIS; GENE EXPRESSION; NUCLEIC ACIDS; RNA; STEARIC ACID; YEAST;

ARACHIDONIC ACIDS; FATTY ACID COMPOSITIONS; ISOLATION AND CHARACTERIZATIONS; MORTIERELLA; MORTIERELLA ALPINA; RNA INTERFERENCE; RNA INTERFERENCES; SATURATED FATTY ACIDS; YEAST SACCHAROMYCES CEREVISIAE; YEAST TRANSFORMANT;

ACIDS;

ARACHIDONIC ACID; BACTERIAL ENZYME; ELONGASE; FATTY ACID; STEARIC ACID; UNCLASSIFIED DRUG;

ENZYME ACTIVITY; FATTY ACID; FUNGUS; GENE EXPRESSION; YEAST;

ARTICLE; ENZYME ISOLATION; GENE EXPRESSION; GENE ISOLATION; MORTIERELLA ALPINA; NONHUMAN; NUCLEOTIDE SEQUENCE; RNA INTERFERENCE; YEAST;

ACETYLTRANSFERASES; AMINO ACID SEQUENCE; ARACHIDONIC ACID; FATTY ACIDS; FUNGAL PROTEINS; GENE EXPRESSION; MOLECULAR SEQUENCE DATA; MORTIERELLA; RNA INTERFERENCE; SACCHAROMYCES CEREVISIAE; SEQUENCE HOMOLOGY, AMINO ACID;

FUNGI; MORTIERELLA; MORTIERELLA ALPINA; SACCHAROMYCES CEREVISIAE;

EID: 56349100520     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-008-1675-z     Document Type: Article

References (19)

1. T Abe E Sakuradani T Ueda S Shimizu 2005 Identification of mutation sites on Δ5 desaturase genes from Mortierella alpina 1S-4 mutants J Biosci Bioeng 99 296 299
2. T Abe E Sakuradani T Asano H Kanamaru S Shimizu 2006 Functional characterization of Δ9 and ω9 desaturase genes in Mortierella alpina 1S-4 and its derivative mutants Appl Microbiol Biotechnol 70 711 719
3. Ausubel FM, Brent R, Kingston RE, Moore DD, Seidman JG, Smith JA, Struhl K (1994) Transformation by electroporation. In: Current protocols in molecular biology. Green and Wiley-Interscience, New York, pp 13.7.5.-13.7.7.
4. JM Fritzler JJ Millership G Zhu 2007 Cryptosporidium parvum long-chain fatty acid elongase Eukaryot Cell 6 2018 2028
5. AE Leonard SL Pereira H Sprecher YS Huang 2004 Elongation of long-chain fatty acids Prog Lipid Res 43 36 54
6. CS Oh DA Toke S Mandala CE Martin 1997 ELO2 and ELO3, homologues of the Saccharomyces cerevisiae ELO1 gene, function in fatty acid elongation and are required for sphingolipid formation J Biol Chem 272 17376 17384
7. JM Parker-Barnes T Das E Bobik AE Leonard JM Thurmond LT Chaung YS Huang P Mukerji 2000 Identification and characterization of an enzyme involved in the elongation of n-6 and n-3 polyunsaturated fatty acids Proc Natl Acad Sci U S A 97 8284 8289
8. P Prasitchoke Y Kaneko T Bamba E Fukusaki A Kobayashi S Harashima 2007 Identification and characterization of a very long-chain fatty acid elongase gene in the methylotrophic yeast, Hansenula polymorpha Gene 391 16 25
9. 9-Fatty acid desaturase from arachidonic acid-producing fungus. Unique gene sequence and its heterologous expression in a fungus, Aspergillus Eur J Biochem 260 208 216
10. E Sakuradani M Kobayashi T Ashikari S Shimizu 1999 Identification of Δ12-fatty acid desaturase from arachidonic acid-producing Mortierella fungus by heterologous expression in the yeast Saccharomyces cerevisiae and the fungus Aspergillus oryzae Eur J Biochem 261 812 820
11. E Sakuradani M Kobayashi S Shimizu 1999 Δ6-Fatty acid desaturase from an arachidonic acid-producing Mortierella fungus. Gene cloning and its heterologous expression in a fungus, Aspergillus Gene 238 445 453
12. E Sakuradani S Shimizu 2003 Gene cloning and functional analysis of a second Δ6-fatty acid desaturase from an arachidonic acid-producing Mortierella fungus Biosci Biotechnol Biochem 67 704 711
13. E Sakuradani T Abe K Iguchi S Shimizu 2005 A novel fungal ω3-desaturase with wide substrate specificity from arachidonic acid-producing Mortierella alpina 1S-4 Appl Microbiol Biotechnol 66 648 654
14. S Takeno E Sakuradani S Murata M Inohara-Ochiai H Kawashima T Ashikari S Shimizu 2004 Establishment of an overall transformation system for an oil-producing filamentous fungus, Mortierella alpina 1S-4 Appl Microbiol Biotechnol 65 419 425
15. S Takeno E Sakuradani S Murata M Inohara-Ochiai H Kawashima T Ashikari S Shimizu 2005 Molecular evidence that the rate-limiting step for the biosynthesis of arachidonic acid in Mortierella alpina is at the level of an elongase Lipids 40 25 30
16. S Takeno E Sakuradani A Tomi M Inohara-Ochiai H Kawashima T Ashikari S Shimizu 2005 Improvement of the fatty acid composition of an oil-producing filamentous fungus, Mortierella alpina 1S-4, through RNA interference with Δ12-desaturase gene expression Appl Environ Microbiol 71 5124 5128
17. DA Toke CE Martin 1996 Isolation and characterization of a gene affecting fatty acid elongation in Saccharomyces cerevisiae J Biol Chem 271 18413 18422
18. H Yamada S Shimizu Y Shinmen 1987 Production of arachidonic acid by Mortierella elongata 1S-5 Agric Biol Chem 51 785 790
19. S Zhang E Sakuradani K Ito S Shimizu 2007 Identification of a novel bifunctional Δ12/Δ15 fatty acid desaturase from a basidiomycete, Coprinus cinereus TD#822-2 FEBS Lett 581 315 319