Establishment of Agrobacterium tumefaciens-mediated transformation of an oleaginous fungus, Mortierella alpina 1S-4, and its application for eicosapentaenoic acid producer breeding

Applied and Environmental Microbiology

Volumn 75, Issue 17, 2009, Pages 5529-5535

  Ando, Akinori ^a   Sumida, Yosuke ^a   Negoro, Hiroaki ^a   Suroto, Dian Anggraini ^a   Ogawa, Jun ^a   Sakuradani, Eiji ^a   Shimizu, Sakayu ^a  

^a KYOTO UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

AGROBACTERIUM TUMEFACIENS-MEDIATED TRANSFORMATION; AUXOTROPHIC MUTANTS; DESATURASE; EICOSAPENTAENOIC ACID; END-PRODUCTS; FILAMENTOUS FUNGI; GENE MANIPULATION; HOST STRAIN; INDUSTRIAL STRAIN; MITOTIC STABILITY; MORTIERELLA ALPINA 1S-4; N-3 FATTY ACIDS; OLEAGINOUS FUNGI; RANDOM POSITION; SELECTABLE MARKER GENES; TRANSFORMANTS;

DNA; FATTY ACIDS; GENE EXPRESSION; NUCLEIC ACIDS;

ACIDS;

ICOSAPENTAENOIC ACID; OMEGA 3 FATTY ACID; OMEGA 6 FATTY ACID; URACIL;

BACTERIUM; BIOTRANSFORMATION; CARBOXYLIC ACID; CATALYSIS; CHROMOSOME; FUNGUS; GENE EXPRESSION; GENETIC ENGINEERING; GENETIC MARKER; INDUSTRIAL PRODUCTION; MUTATION; SPORE;

ARTICLE; AUXOTROPHIC MUTANT; BACTERIAL STRAIN; BREEDING; CATALYSIS; ENZYME SYNTHESIS; FUNGUS; MARKER GENE; MITOSIS; MORTIERELLA ALPINA; MUTAGENESIS; NONHUMAN; PROTEIN EXPRESSION; RHIZOBIUM RADIOBACTER;

CHROMOSOMES, FUNGAL; EICOSAPENTAENOIC ACID; FATTY ACID DESATURASES; FATTY ACIDS; GENE DOSAGE; GENE TRANSFER TECHNIQUES; GENETIC ENGINEERING; GENOMIC INSTABILITY; MORTIERELLA; RECOMBINATION, GENETIC; RHIZOBIUM RADIOBACTER; TRANSFORMATION, GENETIC;

AGROBACTERIUM TUMEFACIENS; FUNGI; MORTIERELLA ALPINA;

EID: 69449083094     PISSN: 00992240     EISSN: 10985336     Source Type: Journal    
DOI: 10.1128/AEM.00648-09     Document Type: Article

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