Purification and characterization of a protein with antifungal, antiproliferative, and HIV-1 reverse transcriptase inhibitory activities from small brown-eyed cowpea seeds

Biotechnology and Applied Biochemistry

Volumn 60, Issue 4, 2013, Pages 393-398

  Tian, Guo Ting ^a   Zhu, Meng Juan ^b   Wu, Ying Ying ^b   Liu, Qin ^b   Wang, He Xiang ^b   Ng, Tzi Bun ^c  

^a INSTITUTE OF MEDICINAL PLANTS   (China)

^b CHINA AGRICULTURAL UNIVERSITY   (China)

^c Prince of Wales Hospital   (Hong Kong)

Author keywords

antifungal protein; antiproliferative; HIV 1 reverse transcriptase; isolation; small brown eyed cowpea seeds; Vigna unguiculata

Indexed keywords

ANTI-PROLIFERATIVE; ANTIFUNGAL PROTEIN; HIV-1 REVERSE TRANSCRIPTASE; ISOLATION; VIGNA UNGUICULATA;

ANTIFUNGAL AGENTS; DISEASES; FUNGI; ONCOLOGY; VIRUSES;

PROTEINS;

BROWN EYED COWPEA SEED PROTEIN; POLYGALACTURONASE; RNA DIRECTED DNA POLYMERASE INHIBITOR; UNCLASSIFIED DRUG; VEGETABLE PROTEIN;

ANTIFUNGAL ACTIVITY; ANTIPROLIFERATIVE ACTIVITY; ARTICLE; BOTRYTIS CINEREA; COWPEA; DNA SYNTHESIS; ENZYME INHIBITION; FUNGUS; FUSARIUM OXYSPORUM; HUMAN IMMUNODEFICIENCY VIRUS 1; IC 50; LEUKEMIA L 1210; LYMPHOMA CELL LINE; MYCOSPHAERELLA ARACHIDICOLA; NUCLEOTIDE SEQUENCE; PLANT SEED; PROTEIN PURIFICATION;

ANTIFUNGAL PROTEIN; ANTIPROLIFERATIVE; HIV-1 REVERSE TRANSCRIPTASE; ISOLATION; SMALL BROWN-EYED COWPEA SEEDS; VIGNA UNGUICULATA;

AMINO ACID SEQUENCE; ANTIFUNGAL AGENTS; ANTINEOPLASTIC AGENTS; CELL LINE, TUMOR; CELL PROLIFERATION; FABACEAE; FUNGI; HIV REVERSE TRANSCRIPTASE; HUMANS; MOLECULAR SEQUENCE DATA; PLANT PROTEINS; REVERSE TRANSCRIPTASE INHIBITORS; SEEDS;

EID: 84883184908     PISSN: 08854513     EISSN: 14708744     Source Type: Journal    
DOI: 10.1002/bab.1102     Document Type: Article

References (40)

1. Pressey, R., (1997) Phytochemistry 44, 1241-1245.
2. Vogelsang, R., and, Barz, W., (1993) Planta 189, 60-69.
3. Lam, Y. W., Wang, H. X., and, Ng, T. B., (2000) Biochem. Biophys. Res. Commun. 279, 74-80.
4. Roberts, W. K., and, Selitrennikoff, C. P., (1986) Biochim. Biophys. Acta 880, 161-170.
5. Thevissen, K., Ferket, K. K., Francois, I. E., and, Cammue, B. P., (2003) Peptides 24, 1705-1712.
6. Wong, J. H., and, Ng, T. B., (2003) Peptides 24, 963-968.
7. Wang, H., and, Ng, T. B., (2000) Biochem. Biophys. Res. Commun. 279, 407-411.
8. Ye, X. Y., and, Ng, T. B., (2002) Life Sci. 71, 1667-1680.
9. Caruso, C., Chilosi, G., Leonardi, L., Bertini, L., Magro, P., Buonocore, V., and, Caporale, C., (2001) Phytochemistry 58, 743-750.
10. Parkash, A., Ng, T. B., and, Tso, W. W., (2002) Peptides 23, 1019-1024.
11. Ye, X. Y., Ng, T. B., Tsang, P. W., and, Wang, J., (2001) J. Protein Chem. 20, 367-375.
12. Wang, S. Y., Wu, J. H., Ng, T. B., Ye, X. Y., and, Rao, P. F., (2004) Peptides 25, 1235-1242.
13. Iijima, R., Kurata, S., and, Natori, S., (1993) J. Biol. Chem. 268, 12055-12061.
14. Oeser, B., Heidrich, P. M., Muller, U., Tudzynski, P., and, Tenberge, K. B., (2002) Fungal Genet. Biol. 36, 176-186.
15. Poinssot, B., Vandelle, E., Bentejac, M., Adrian, M., Levis, C., Brygoo, Y., Garin, J., Sicilia, F., Coutos-Thevenot, P., and, Pugin, A., (2003) Mol. Plant Microbe Interact. 16, 553-564.
16. De Lorenzo, G., D'Ovidio, R., and, Cervone, F., (2001) Annu. Rev. Phytopathol. 39, 313-335.
17. Laemmli, U. K., and, Favre, M., (1973) J. Mol. Biol. 80, 575-599.
18. Lam, S. S., Wang, H., and, Ng, T. B., (1998) Biochem. Biophys. Res. Commun. 253, 135-142.
19. Ma, D. Z., Wang, H. X., and, Ng, T. B., (2009) Peptides 30, 2089-2094.
20. Wang, H., and, Ng, T. B., (2001) Biochem. Biophys. Res. Commun. 289, 120-124.
21. Ferrari, S., Vairo, D., Ausubel, F. M., Cervone, F., and, De Lorenzo, G., (2003) Plant Cell 15, 93-106.
22. Ng, T. B., Au, T. K., Lam, T. L., Ye, X. Y., and, Wan, D. C., (2002) Life Sci. 70, 927-935.
23. Kemp, G., Bergmann, C. W., Clay, R., Van der Westhuizen, A. J., and, Pretorius, Z. A., (2003) Mol. Plant Microbe Interact. 16, 955-961.
24. Sathiyaraj, G., Srinivasan, S., Subramanium, S., Kim, Y. J., Kim, Y. J., Kwon, W. S., and, Yang, D. C., (2010) Mol. Biol. Rep. 37, 3445-3454.
25. Brouwer, J. N., Glaser, D., Hard, A. S. C., Hellekant, G., Ninomiya, Y., and, Van der Wel, H., (1983) J. Physiol. 337, 221-240.
26. Ye, X. Y., Wang, H. X., and, Ng, T. B., (2000) Life Sci. 67, 775-781.
27. van der Wel, H., and, Loeve, K,. (1972) Eur. J. Biochem. 31, 221-225.
28. Lam, S. K., and, Ng, T. B., (2009) Phytomedicine 16, 172-180.
29. Hartweck, L. M., Vogelzang, R. D., and, Osborn, T. C., (1991) Plant Physiol. 97, 204-211.
30. Benjannet, S., Seidah, N. G., Routhier, R., and, Chrétien, M., (1980) Nature 285, 415-416.
31. O'Donohue, T. L., Handelmann, G. E., Loh, Y. P., and, Olton, D. S., (1981) Peptides 2, 101-104.
32. McLean, C., and, Lowry, P. J., (1981) Nature 290, 341-343.
33. Park, S. C., Lee, J. R., Shin, S. O., Jung, J. H., Lee, Y. M., Son, H., Park, Y., Lee, S. Y., and, Hahm, K. S., (2007) J. Agric. Food Chem. 55, 5277-5281.
34. Bottcher, M., and, Grosse, F., (1997) Nucleic Acids Res. 25, 1709-1714.
35. Li, M,., Wang, H. X., and, Ng, T. B., (2011) Protein Pept. Let t. 18, 594-600.
36. Ma, D. Z., Wang, H. X., and, Ng, T. B., (2009) Peptides 30, 2089-2094.
37. Wu, X., Sun, J., Zhang, G. Q., Wang, H. X., and, Ng, T. B., (2011) Phytomedicine 18, 104-109.
38. Zhu, M. J., Zhang, G. Q., Wang, H. X., and, Ng, T. B., (2011) Protein J. 30, 240-246.
39. Zhao, Y. C., Zhang, G. Q., Ng, T. B., and, Wang, H. X., (2011) J. Microbiol. 49, 803-808.
40. Sun, J,., Wang, H. X., and, Ng, T. B., (2010) J. Biomed. Biotechnol. 2012, 219793.