Isolation of a Polygalacturonase-Inhibiting Protein (PGIP) from Wheat

Molecular Plant-Microbe Interactions

Volumn 16, Issue 11, 2003, Pages 955-961

  Kemp, Gabré ^a,b   Bergmann, Carl W ^b   Clay, Ron ^c   Van Der Westhuizen, Amie J ^a   Pretorius, Zacharias A ^a  

^a UNIVERSITY OF THE FREE STATE   (South Africa)

^b UNIVERSITY OF GEORGIA   (United States)

^c Gainesville College   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

COCHLIOBOLUS SATIVUS; FUNGI; POACEAE; TRITICUM AESTIVUM;

EID: 0142183520     PISSN: 08940282     EISSN: None     Source Type: Journal    
DOI: 10.1094/MPMI.2003.16.11.955     Document Type: Article

References (39)

1. Abu-Goukh, A. A., Greve, L. C., and Labavitch, J. M. 1983. Purification and partial characterization of 'Bartlett' pear fruit polygalacturonase inhibitors. Physiol. Plant Pathol. 23:111-122.
2. Albersheim, P., and Anderson, A. J. 1971. Proteins from plant cell walls inhibit polygalacturonase secreted by plant pathogens. Proc. Natl. Acad. Sci. U.S.A. 68:1815-1819.
3. Albersheim, P., and Anderson-Prouty, A. J. 1975. Carbohydrates, proteins, cell surfaces, and the biochemistry of pathogenesis. Ann. Rev. Plant Physiol. 26:31-52.
4. Barmore, C. R., and Nguyen, T. K. 1985. Polygalacturonase inhibition in rind of Valencia oranges infected with Diplodia natalensis. Phytopathology 75:446-449.
5. Bergmann, C. W., Ito, Y., Singer, D., Albersheim, P., and Darvill, A. G. 1994. Polygalacturonase-inhibiting protein accumulates in Phaseolus vulgaris L. in response to wounding, elicitors and fungal infection. Plant J. 5:625-634.
6. Bradford, M. M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72:248-253.
7. Caprari, C., Richter, A., Bergmann, C. W., Lo Cicero, S., Salvi, G., Cervone, F. and De Lorenzo, G. 1993. Cloning and characterization of a gene encoding the endopolygalacturonase of Fusarium moniliforme. Mycol. Res. 97:497-505.
8. Cervone, F. 2003. Structure-function and molecular studies on fungal polygalacturonases and their inhibitors PGIPs. Pages 293-297 in: Advances in Pectin and Pectinases Research. F. Voragen, H. Schols, and R. Visser, eds. Kluwer Academic Publishers, Dordrecht, The Netherlands.
9. Cervone, F., De Lorenzo, G., Degrà, L., Salvi, G., and Bergami, M. 1987. Purification and characterization of a polygalacturonase-inhibiting protein from Phaseolus vulgaris L. Plant Physiol. 85:631-637.
10. Cervone, F., De Lorenzo, G., Pressey, R., Darvill, A. G., and Albersheim, P. 1989. Host-pathogen interactions. XXXIII. A plant protein converts a fungal pathogenesis factor into an elicitor of plant defense responses. Plant Physiol. 90:542-545.
11. Clay, R. P., Bergmann, C. W., and Fuller, M. S. 1997. Isolation and characterization of an endopolygalacturonase from Cochliobolus sativus and a cytological study of fungal penetration of barley. Phytopathology 87:1148-1159.
12. Cook, B. J., Clay, R. P., Bergmann, C. W., Albersheim, P., and Darvill, A. G. 1999. Fungal polygalacturonases exhibit different substrate degradation patterns and differ in their susceptibilities to polygalacturonase-inhibiting proteins. Mol. Plant-Microbe Interact. 12:703-711.
13. Cooper, R. M. 1984. The role of cell wall degrading enzymes in infection and damage. Pages 13-28 in: Plant Disease, Infection, Damage and Loss. R. K. S. Wood and G. J. Jellis, eds., Blackwell Scientific, Oxford.
14. Coutinho, P. M., and Henrissat, B. 1999. The modular structure of cellulases and other carbohydrate-active enzymes: An integrated database approach. Pages 15-23 in: Genetics Biochemistry and Ecology of Cellulose Degradation. K. Ohmiya, K. Hayashi, K. Sakka, Y. Kobayashi, S. Karita, and T. Kimura, eds. Uni Publishers, Tokyo.
15. Darvill, A. G., McNeil, M., Albersheim, P., and Delmer, D. P. 1980. The primary cell walls of flowering plants. Pages 91-162 in: The Biochemistry of Plants. Vol. 1. The Plant Cell. N. E. Tolbert, ed. Academic Press, New York.
16. De Lorenzo, G., Cervone, F., Bellincampi, D., Caprari, C., Clark, A. J., Desiderio, A., Devoto, A., Forrest, R., Leckie, F., Nuss, L., and Salvi, G. 1994. Polygalacturonase, PGIP and oligogalacturonides in cell-cell communication. Biochem. Soc. Trans. 22:396-399.
17. Dunbar, B. S., and Schwoebel, E. D. 1990. Preparation of polyclonal antibodies. Pages 633-670 in: Guide to Protein Purification, Methods in Enzymology. Vol. 182. M. P. Deutscher, ed. Academic Press, New York.
18. English, P. D., Maglothin, A., Keegstra, K., and Albersheim, P. 1972. A cell wall-degrading endopolygalacturonase secreted by Colletrotrichum lindemuthianum. Plant Physiol. 49:293-297.
19. Favaron, F. 2001. Gel detection of Allium porrum polygalacturonase- inhibiting protein reveals a high number of isoforms. Physiol. Mol. Plant Pathol. 58:239-245.
20. Favaron, F., D'Ovidio, R., Porceddu, E., and Alghisi, P. 1994. Purification and molecular characterization of a soybean polygalacturonase- inhibiting protein. Planta 195:80-87.
21. Favaron, F., Castiglioni, C., D'Ovidio, R., and Alghisi, P. 1997. Polygalacturonase inhibiting proteins from Allium porrum L. and their role in plant tissue against fungal endo-polygalacturonases. Physiol. Mol. Plant Pathol. 50:403-417.
22. Fritig, B., Heitz, T., and Legrand, M. 1998. Antimicrobials proteins in induced plant defense. Curr. Opin. Immunol. 10:16-22.
23. James, J. T., and Dubery, I. A. 2001. Inhibition of polygalacturonase from Verticillium dahliae by a polygalacturonase inhibiting protein from cotton. Phytochemistry 57:149-156.
24. Janick, J. 2001. New crops for the 21st century. Pages 307-327 in: Crop Science: Progress and Prospects. J. Nösberger, H. H. Geiger, and P. C. Struik, eds. CABI Publishing, Wallingford, U.K.
25. Kemp, G., Botha, A.-M., Kloppers, F. J., and Pretorius, Z. A. 1999. Disease development and β-1,3-glucanase expression following leaf rust infection in resistant and susceptible near-isogenic wheat seedlings. Physiol. Mol. Plant Pathol. 55:45-92.
26. King, D., Bergmann, C., Orlando, R., Benen, J. A. E., Kester, H. C., and Visser, J. 2002. Use of amide exchange mass spectrometry to study conformational changes within the endopolygalacturonase II-homogalacturonan- polygalacturonase inhibiting protein system. Biochemistry 41:10225-10233.
27. Laemmli, U. K. 1970. Cleavage of structural proteins during the assembly of the head of the bacteriophage T4. Nature 227:680-685.
28. Leckie, F., Mattei, B., Capodicasa, C., Hemmings, A., Nuss, L., Aracri, B., De Lorenzo, G., and Cervone, F. 1999. The specificity of polygalacturonase-inhibiting protein (PGIP): A single amino acid substitution in the solvent-exposed β-strand/β-turn region of the leucine-rich repeats (LRRs) confers a new recognition capability. EMBO (Eur. Mol. Biol. Organ.) J. 18:2352-2363.
29. Peretto, R., Favaron, F., Bettini, V., De Lorenzo, G., Marini, S., Alghisi, P., Cervone, F., and Bonfante, P. 1992. Expression and localization of polygalacturonase during the outgrowth of lateral roots in Allium porrum L. Planta 188:164-172.
30. Reynolds, E. S. 1963. The use of lead citrate at high pH as an electron-opaque stain in electron microscopy. J. Cell Biol. 17:208-210.
31. Rohringer, R., Ebrahim-Nesbat, F., and Wolf, G. 1983. Proteins in intercellular washing fluids from leaves of barley (Hordeum vulgare L.). J. Exp. Bot. 34:1589-1605.
32. Salvi, G., Giarrizzo, F., De Lorenzo, G., and Cervone, F. 1990. A polygalacturonase-inhibiting protein in the flowers of Phaseolus vulgaris L. J. Plant Physiol. 136:513-518.
33. Shevchenko, A., Wilm, M., Vorm, O., and Mann, M. 1996. Mass spectrometric sequencing of proteins silver-stained polyacrylamide gels. Anal. Chem. 68:850-858.
34. Staskawicz, B. J., Ausubel, F. M., Baker, B. J., Ellis, J. G., and Jones, J. D. G. 1995. Molecular genetics of plant disease resistance. Science 268:661-667.
35. Stotz, H. U., Bishop, J. G., Bergmann, C. W., Koch, M., Albersheim, P., Darvill, A. G., and Labavitch, J. M. 2000. Identification of target amino acids that affect interactions of fungal polygalacturonases and their plant inhibitors. Physiol. Mol. Plant Pathol. 56:117-130.
36. Towbin, H., Staehelin, T., and Gordon, I. 1979. Electrophoretic transfer of proteins from acrylamide gels to nitrocellulose sheets: Procedure and some applications. Proc. Natl. Acad. Sci. U.S.A. 76:4350-4354.
37. Wiese, M. V. 1987. Compendium of Wheat Diseases. 2nd ed. The American Phytopathological Society, St. Paul, MN, U.S.A.
38. York, W. S., Darvill, A. G., McNeil, M., Stevenson, T. T., and Albersheim, P. 1985. Isolation and characterization of plant cell walls and plant cell wall components. Methods Enzymol. 118:3-40.
39. Zheng, Y., Yang, Z., Li, J., Yu, L., and Wu, W. 1994. Purification of polygalacturonase inhibiting protein from Triticum aestivum L. Acta Biochim. Biophys. Sinica 26:555-558.