The Use of fish-Derived cell lines for investigation of environmental contaminants: An update following OECD's fish toxicity testing framework no. 171

Current Protocols in Toxicology

Volumn 1, Issue SUPPL.56, 2013, Pages

  Dayeh, Vivian R ^a   Bols, Niels C ^a   Tanneberger, Katrin ^b   Schirmer, Kristin ^b   Lee, Lucy E J ^c  

^a UNIVERSITY OF WATERLOO   (Canada)

^b SWISS FEDERAL INSTITUTE OF AQUATIC SCIENCE AND TECHNOLOGY   (Switzerland)

^c UNIVERSITY OF THE FRASER VALLEY   (Canada)

Author keywords

Alamar blue; Cell viability; CFDA AM; Fish cell lines; In vitro toxicity; Neutral red; Toxicity test; Uorescent indicator dyes

Indexed keywords

5 CARBOXYFLUORESCEIN DIACETATE ACETOXYMETHYL ESTER; ALAMAR BLUE; FLUORESCENT DYE; INDICATOR; NEUTRAL RED; UNCLASSIFIED DRUG; WATER POLLUTANT;

ANIMAL CELL; ARTICLE; CELL ASSAY; CELL CULTURE; CELL FUNCTION; CELL LINE; CELL MEMBRANE; CELL MEMBRANE INTEGRITY; CELL METABOLISM; CELL VIABILITY; CELLULAR PARAMETERS; CYTOTOXICITY; DATA PROCESSING; FISH; FISH DERIVED CELL LINE; FLUOROMETRY; LYSOSOME; NONHUMAN; PRIORITY JOURNAL; SALMONID; TOXICITY TESTING; WATER POLLUTION INDICATOR; ANALYSIS; ANIMAL; ANIMAL TESTING ALTERNATIVE; CELL SURVIVAL; DRUG EFFECTS; FOOD CONTAMINATION; METHODOLOGY; PHYSIOLOGY; PROCEDURES; SEA FOOD; TOXICITY; WATER POLLUTANT; DRUG EFFECT;

ANIMAL USE ALTERNATIVES; ANIMALS; CELL LINE; CELL SURVIVAL; FOOD CONTAMINATION; RESEARCH DESIGN; SALMONIDAE; SEAFOOD; TOXICITY TESTS; WATER POLLUTANTS, CHEMICAL;

EID: 84883154221     PISSN: 19349254     EISSN: 19349262     Source Type: Journal    
DOI: 10.1002/0471140856.tx0105s56     Document Type: Article

References (41)

1. Blagosklonny,M.V. 2000. Cell death beyond apop-tosis. Leukemia 14:1502-1508.
2. Bols, N.C., Mosser, D.D., Steels, G.B. 1992. Temperature studies and recent advances with fish cells in vitro. Comp. Biochem. Physiol. 103A:1-14.
3. Borenfreund, E. and Puerner, J.A. 1984. A simple quantitative procedure usingmonolayer cultures for cytotoxicity assays. J. Tissue Cult. Methods 9:7-12.
4. Brennan, L.M., Widder, M.W., Lee, L.E., van der Schalie, W.H. 2012. Long-term storage and impedance-based water toxicity testing capabil-ities of uidic biochips seeded with RTgill-W1 cells. Toxicol. In Vitro 26:736-745.
5. Clemons, J.H., van den Heuvel, M.R., Stegeman, J.J., Dixon, D.G., Bols, N.C. 1994. A com-parison of toxic equivalent factors for selected dioxin and furan congeners derived using fish and mammalian liver cell lines. Can. J. Fish. Aquat. Sci. 51:1577-1584.
6. Dayeh, V.R., Schirmer, K., Bols, N.C. 2009. Ammonia-containing industrial efuents lethal to rainbow trout vacuolization and neutral red uptake in the rainbow trout gill cell line, RTgill-W1. ATLA 37:77-87.
7. Essig-Marcello, J.S. and van Buskirk, R.G. 1990. A double-label in situ cytotoxicity assay using the uorescent probes neutral red and BCECF-AM. In Vitro Toxicol. 3:219-227.
8. Evans, S.M., Casartelli, A., Herreros, E., Minnick, D.T., Day, C., George, E., Westmoreland, C. 2001. Development of a high throughput in vitro toxicity screen predictive of high acute in vivo toxic potential. Toxicol. In Vitro 15:579-584
9. Flint, O.P. 1990. In vitro toxicity testing: Pur-pose, validation and strategy. Altern. Lab. Anim. 18:11-18.
10. Haugland, R.P. 1996. Handbook of Fluorescent Probes and Research Chemicals. pp. 365-398, 549. Molecular Probes, Inc., Eugene, Oregon.
11. Hestermann, E.V., Stegemann, J.J., Hahn,M.E. 2000. Serum alters the uptake and relative po-tencies of halogenated aromatic hydrocarbons in cell culture bioassays. Toxicol. Sci. 53:316-325.
12. Kramer, N.I., Busser, F.J.M., Oosterwijk, M.T.T., Schirmer, K., Escher, B.I., Hermens, J.L.M. 2010. Development of a partition-controlled dosing system for cell assays. Chem. Res. Toxicol. 23:1806-1814.
13. Lee, L.E.J., Dayeh, V.R., Schirmer, K., Bols, N.C. 2009. Applications and potential uses of fish gill cell lines: Examples with RTgill-W1. In Vitro Cell Dev. Biol. Anim. 45:127-134.
14. Leibovitz, A. 1963. The growth and maintenance of tissue-cell cultures in free gas exchange with the atmosphere. Am. J. Hygiene 78:173-180.
15. O'Brien, J., Wilson, I., Orton, T., Pognan, F. 2000. Investigation of the alamar blue (re-sazurin) uorescent dye for the assessment of mammalian cell cytotoxicity. Eur. J. Biochem. 267:5421-5426.
16. O'Connor, S.,McNamara, L., Swerdin,M., Van Buskirk, R.G. 1991. Multiuorescent assays re-veal mechanisms underlying cytotoxicity-phase ICFTAcompounds. In Vitro Toxicol. 4:197-207.
17. O'Donnell-Tormey, J.,Nathan, C.F., Lanks,K.,De-Boer, C.J., de la Harpe, J. 1987. Secretion of pyruvate: An antioxidant defense ofmammalian cells. J. Exp. Med. 165:500-514.
18. OECD. 2012. Fish Testing Framework. Series on Testing and Assessment. No 171. http://search. oecd.org/ofcialdocuments/displaydocumentpdf /?cote=ENV/JM/MONO(2012)16&doclanguage=en.
19. Oris, J.T. and Giesy, J.P. 1987. The photo-induced toxicity of polycyclic aromatic hydrocarbons to larvae of the fathead minnow (Pimephales promelas). Chemosphere 16:1395-1404.
20. Parkinson, C. and Agius, C. 1987. The effects of solvents on the toxicity of DDT to fish cells. Arch. Toxicol. Suppl. 11:240-242.
21. Reader, S.J., Blackwell, V., O'Hara, R., Clothier, R.H., Grifn, G., Balls, M. 1990. Neutral red release from pre-loaded cells as an in vitro approach to testing for eye irritancy potential. Toxicol. In Vitro 4:264-266.
22. Rotman, B. and Papermaster, B.W. 1966. Mem-brane properties of living mammalian cells as studied by enzymatic hydrolysis of uorogenic esters. Proc. Natl. Acad. Sci. U.S.A. 55:134-141.
23. Schirmer, K., Chan, A.G.J., Greenberg, B.M., Dixon,D.G., Bols,N.C. 1997.Methodology for demonstrating and measuring the photocy-totoxicity of uoranthene to fish cells in culture. Toxicol. In Vitro 11:107-119.
24. Schirmer,K.,Greenberg,B.,Dixon,D.G., Bols, N.C. 1998. Ability of 16 priority PAHs to be directly cytotoxic to a cell line from the rainbow trout gill. Toxicology 127:129-141.
25. Schirmer, K., Chan, A.G.J., Greenberg, B.M., Dixon,D.G., Bols,N.C. 1998.Ability of 16 priority PAHs to be photocytotoxic to a cell line fromthe rainbow trout gill. Toxicology 127:143-155.
26. Schirmer, K., Chan, A.G.J., Bols, N.C. 2000. Transitorymetabolic disruption and cytotoxicity elicited by benzo[a]pyrene in two cell lines from rainbow trout liver. J. Biochem. Mol. Toxicol. 14:262-276.
27. Schnell, S., Kawano, A., Porte, C., Lee, L.E.J., Bols, N.C. 2009. Effects of ibuprofen on the viability and proliferation of rainbow trout liver cell lines and potential problems and inter-actions in effect assessment. Environ. Toxicol. 24:157-165.
28. Schrek, R. 1965. In vitro methods for measuring viability and vitality of lymphocytes exposed to 45{ring operator} C, 47{ring operator} C and 50{ring operator} C. Cryobiology 3:322-328.
29. Segner, H., Behrens, A., Joyce, E.M., Schirmer, K., Bols, N.C. 2000. Transient induction of 7-ethoxyresorun-o-deethylase (EROD) activ-ity by medium change in the rainbow trout liver cell line, RTL-W1. Mar. Environ. Res. 50:489-493.
30. Shaw, A.J. 1994. Dening cell viability and cyto-toxicity. A.T.L.A. 22:124-126.
31. Smith, K.E.C., Oostingh, G.J., Mayer, P. 2010. Passive dosing for producing dened and con-stant exposure of hydrophobic organic com-pounds during in vitro toxicity tests. Chem. Res. Toxicol. 23:55-65.
32. Tanneberger, K., Rico-Rico, A., Kramer, N.I., Busser, F.J.M., Hermens, J.L.M., Schirmer, K. 2010. Effects of solvents and dosing proce-dure on chemical toxicity in cell-based in vitro assays. Environ. Sci. Technol. 44:4775-4781.
33. Tanneberger, K., Kn̈obel, M., Busser, F.J.M., Sinnige, T.L., Hermens, J.L.M., Schirmer, K. 2013. Predicting fish acute toxicity using a fish gill cell line-based toxicity assay. Environ. Sci. Technol. 47:1110-1119.
34. Yu, K.O., Fisher, J.W., Burton, G.A., Tillitt, D.E. 1997. Carrier effects of dosing the H4IIE cells with 3,3,4,4-tetrachlorobiphenyl (PCB 77) in dimethyl sulfoxide or isooctane. Chemo-sphere 35:895-904.
35. Zhang, S.Z., Lipsky, M.M., Trump, B.F., Hsu, I.C. 1990. Neutral red (NR) assay for cell vi-ability and xenobiotic-induced cytotoxicity in primary cultures of human and rat hepatocytes. Cell. Biol. Toxicol. 6:210-234
36. Bols, N.C. and Lee, L.E.J. 1994. Cell lines: Avail-ability, propagation and isolation. In Biochem-istry and Molecular Biology of Fishes, vol. 3. (P.W. Hochachka andT.P. Mommsen, eds.) pp. 145-159. Elsevier Science, Amsterdam.
37. Ganassin, R.C., Schirmer, K., Bols, N.C. 2000. Cell and tissue culture. In The Laboratory Fish (G.K. Ostrander, ed.) pp. 631-651. Academic Press, San Diego.
38. Babich, H and Borenfreund, E. 1991. Cytotoxicity and genotoxicity assays with cultured fish cells: Areview. Toxicol. In Vitro 5:91-100.
39. Bols, N.C., Dayeh, V.R., Lee, L.E.J. and Schirmer, K. 2005. Use of fish cell lines in toxicology of fish. In Biochemistry and Molecular Biology of Fishes-Environmental Toxicology. Vol. 6. (T.W. Moon andT. P. Mommsen, eds) pp. 43-84. Else-vier Science, Amsterdam.
40. Fent, K. 2001. Fish cell lines as versatile tools in ecotoxicology: Assessment of cytotoxicity, cy-tochrome P4501A induction potential and estro-genic activity of chemicals and environmental samples. Toxicol. In Vitro 15:477-488.
41. Schirmer, K. 2006. Proposal to improve vertebrate cell cultures to establish them as substitutes for the regulatory testing of chemicals and efuents using fish. Toxicology 224:163-183