Finding the same needles in the haystack? A comparison of phosphotyrosine peptides enriched by immuno-affinity precipitation and metal-based affinity chromatography

Journal of Proteomics

Volumn 91, Issue , 2013, Pages 331-337

  Di Palma, Serena ^a,b,c   Zoumaro Djayoon, Adja ^a,b   Peng, Mao ^a,b   Post, Harm ^a,b   Preisinger, Christian ^a,b,d   Munoz, Javier ^a,b   Heck, Albert J R ^a,b  

^a UTRECHT UNIVERSITY   (Netherlands)

^b NETHERLANDS PROTEOMICS CENTRE   (Netherlands)

^c UNIVERSITY OF ZURICH   (Switzerland)

^d UNIVERSITY HOSPITAL RWTH AACHEN   (Germany)

Author keywords

Hela cells; Immuno affinity enrichment; K562 cells; Phosphoproteomics; Ti4+ IMAC; Tyrosine phosphorylation

Indexed keywords

PHOSPHOPEPTIDE; PHOSPHOTYROSINE;

AFFINITY CHROMATOGRAPHY; AMINO ACID SEQUENCE; ARTICLE; DATA PROCESSING; FEMALE; HELA CELL; HUMAN; HUMAN CELL; HYDROPHILIC INTERACTION CHROMATOGRAPHY; IMMUNO AFFINITY PRECIPITATION; IMMUNOPRECIPITATION; IN VITRO STUDY; PEPTIDE ANALYSIS; PRIORITY JOURNAL; PROTEIN PHOSPHORYLATION;

HELA CELLS; IMMUNO-AFFINITY ENRICHMENT; K562 CELLS; PHOSPHOPROTEOMICS; TI(4+)-IMAC; TYROSINE PHOSPHORYLATION;

CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, LIQUID; HELA CELLS; HUMANS; IMMUNOPRECIPITATION; K562 CELLS; METALS; PEPTIDES; PHOSPHOPEPTIDES; PHOSPHOPROTEINS; PHOSPHORYLATION; PHOSPHOTYROSINE; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEOMICS; SIGNAL TRANSDUCTION; TYROSINE;

EID: 84882954283     PISSN: 18743919     EISSN: 18767737     Source Type: Journal    
DOI: 10.1016/j.jprot.2013.07.024     Document Type: Article

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