Accurate Detection and Quantification of the Fish Viral Hemorrhagic Septicemia virus (VHSv) with a Two-Color Fluorometric Real-Time PCR Assay

PLoS ONE

Volumn 8, Issue 8, 2013, Pages

  Pierce, Lindsey R ^a   Willey, James C ^b   Palsule, Vrushalee V ^a   Yeo, Jiyoun ^b   Shepherd, Brian S ^c   Crawford, Erin L ^b   Stepien, Carol A ^a  

^a UNIVERSITY OF TOLEDO   (United States)

^b MEDICAL COLLEGE OF OHIO   (United States)

^c UNIVERSITY OF WISCONSIN MILWAUKEE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ACCURACY; AMEIURUS NEBULOSUS; ARTICLE; CONTROLLED STUDY; FALSE NEGATIVE RESULT; FISH; FLUOROMETRY; LARGEMOUTH BASS; MICROPTERUS; NONHUMAN; NUCLEOTIDE SEQUENCE; QUALITY CONTROL; QUANTITATIVE ANALYSIS; REAL TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; VIRAL HEMORRHAGIC SEPTICEMIA VIRUS; VIRAL HEMORRHAGIC SEPTICEMIA VIRUS INFECTION; VIRUS DETECTION; VIRUS INFECTION;

ANIMALS; FISH PROTEINS; FLUOROMETRY; GENES, VIRAL; HEMORRHAGIC SEPTICEMIA, VIRAL; LIMIT OF DETECTION; MOLECULAR DIAGNOSTIC TECHNIQUES; NOVIRHABDOVIRUS; PERCIFORMES; REAL-TIME POLYMERASE CHAIN REACTION; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION;

EID: 84882715462     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0071851     Document Type: Article

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