Development and validation of a real-time PCR assay for the quantification of Verticillium dahliae in potato

Plant Disease

Volumn 97, Issue 5, 2012, Pages 608-618

  Pasche, J S ^a   Mallik, I ^a   Anderson, N R ^b   Gudmestad, N C ^a  

^a NORTH DAKOTA STATE UNIVERSITY   (United States)

^b PURDUE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

EID: 84876024076     PISSN: 01912917     EISSN: None     Source Type: Journal    
DOI: 10.1094/PDIS-06-12-0554-RE     Document Type: Article

References (70)

1. Atallah, Z. K., Bae, J., Jansky, S. H., Rouse, D. I., and Stevenson, W. R. 2007. Multiplex real-time quantitative PCR to detect and quantify Verticillium dahliae colonization in potato lines that differ in response to Verticil-lium wilt. Phytopathology 97:865-872.
2. Atallah, Z. K., and Stevenson, W. R. 2006. A methodology to detect and quantify five pathogens causing potato tuber decay using real-time quantitative PCR. Phytopathology 96:1037-1045.
3. Bae, J., Atallah, Z. K., Jansky, S. H., Rouse, D. I., and Stevenson, W. R. 2007. Colonization dynamics and spatial progression of Verticillium dahliae in individual stems of two potato cultivars with differing responses to potato early dying. Plant Dis. 91:1137-1141.
4. Bae, J., Halterman, D., and Jansky, S. 2008. Development of a molecular marker associated with Verticillium wilt resistance in diploid interspecific potato hybrids. Mol. Breed. 22:61-69.
5. Berbegal, M., Ortega, A., Garcia-Jimenez, J., and Armengol, J. 2007. Inoculum density-disease development relationship in Verticillium wilt of Artichoke caused by Verticillium dahliae. Plant Dis. 91:1131-1136.
6. Bowers, J. H., Nameth, S. T., Riedel, R. M., and Rowe, R. C. 1996. Infection and colonization of potato roots by Verticillium dahliae as affected by Pratylenchus penetrans and P. crenatus. Phytopathology 86:614-621.
7. Busch, L. V, and Edgington, V 1967. Correlation of photoperiod with tu-berization and susceptibility to Verticillium albo-atrum. Can. J. Bot. 45:691-693.
8. Bustin, S. A., and Nolan, T. 2004. Pitfalls of quantitative real-time reverse-transcription polymerase chain reaction. J. Biomol. Tech. 15:155-166.
9. Concibido, V C, Secor, G. A., and Jansky, S. H. 1994. Evaluations of resistance to Verticillium wilt in diploid, wild potato interspecific hybrids. Eu-phytica76:145-152.
10. Corsini, D. L., Pavek, J. J., and Davis, J. R. 1988. Verticillium wilt resistance in noncultivated tuber-bearing Solarium species. Plant Dis. 72:148-151.
11. Dan, H., Ali-Khan, S. T., and Robb, J. 2001. Use of quantitative PCR diagnostics to identify tolerance and resistance to Verticillium dahliae in potato. Plant Dis. 85:700-705.
12. Davis, J. R. 1985. Approaches to control of potato early dying caused by Verticillium dahliae. Am. Potato J. 62:177-185.
13. Davis, J. R. 2009. Evidence that two Verticillium-resistant potato varieties have the potential to enhance soil suppressiveness toward Verticillium wilt. (Abstr.) Am. J. Potato Res. 86:140.
14. Davis, J. R., and Huisman, O. C. 2001. Verticillium wilt. Pages 45-46 in: Compendium of Potato Diseases. W. R. Stevenson, R. Loria, G. D. Franc, and D. P. Weingartner, eds. American Phytopathological Society, St. Paul, MN.
15. Davis, J. R., Huisman, O. C, Everson, D. O., and Schneider, A. T. 2001. Verticillium wilt of potato: a model of key factors related to disease severity and tuber yield in southeastern Idaho. Am. J. Potato Res. 78:291-300.
16. Davis, J. R., Pavek, J. J., and Corsini, D. L. 1983. A sensitive method for quantifying Verticillium dahliae colonization in plant tissue and evaluating resistance among potato genotypes. Phytopathology 73:1009-1014.
17. Davis, J. R., and Sorenson, L. H. 1986. Influence of soil solarization at moderate temperatures on potato genotypes with differing resistance to Verticillium dahliae. Phytopathology 76:1021-1026.
18. Davis, J. R., Sorensen, L. H., Stark, J. C, and Westermann, D. T. 1990. Fertility and management practices to control Verticillium wilt of the Russet Burbank potato. Am. Potato J. 67:55-65.
19. Dobinson, K. F, Grant, S. J., and Kang, S. 2004. Cloning and targeted disruption, via Agrobacterium tumefaciens-mediated transformation, of a trypsin protease gene from the vascular wilt fungus Verticillium dahliae. Curr. Genet. 45:104-110.
20. Dung, J. K. S., Ingram, J. T., Cummings, T. F, and Johnson, D. A. 2012. Impact of seed lot infection on the development of black dot and Verticillium wilt of potato in Washington. Plant Dis. 96:1179-1184.
21. Dung, J. K. S., and Johnson, D. A. 2012. Roles of infected seed tubers and soilborne inoculum on Verticillium wilt of 'Russet Burbank' potato. Plant Dis. 96:379-383.
22. Duressa, D., Rauscher, G., Koike, S. T., Mou, B., Hayes, R. J., Maruthacha-lam, K., Subbarao, K. V, and Klosterman, S. J. 2012. A real-time PCR assay for detection and quantification of Verticillium dahliae in spinach seed. Phytopathology 102:443-451.
23. Farley, J. D. 1972. A selective medium for assay of Colletotrichum coc-codes in soil. Phytopathology 62:1288-1293.
24. Hoyos, G. P., Lauer, F I., and Anderson, N. A. 1993. Early detection of Verticillium wilt resistance in a potato breeding program. Am. Potato J. 70:535-541.
25. Hu, X., Nazar, R. N., and Robb, J. 1993. Quantification of Verticillium biomass in wilt disease development. Physiol. Mol. Plant Pathol. 42:23-36.
26. Huisman, O. C, and Gerik, J. S. 1989. Dynamics of colonization of plant roots by Verticillium dahliae and other fungi. Pages 1-17 in: Vascular Wilt Diseases of Plants: Basic Studies and Control. E. C. Tjamos and C. H. Beckman, eds. Springer-Verlag, Berlin.
27. Hunter, D. E., Darling, H. M., Stevenson, F J., and Cunningham, C. E. 1968. Inheritance of resistance to Verticillium wilt in Wisconsin. Am. Potato J. 45:72-78.
28. Jansky, S. H. 2009. Identification of Verticillium wilt resistance in U.S. potato breeding programs. Am. J. Potato Res. 86:504-512.
29. Jansky, S. H., and Miller, J. C. 2010. Evaluation of Verticillium wilt resistance in Russet Norkotah and six strain selections. Am. J. Potato Res. 87:492-496.
30. Jansky, S., and Rouse, D. I. 2000. Identification of potato interspecific hybrids resistant to Verticillium wilt and determination of criteria for resistance assessment. Potato Res. 43:239-251.
31. Jansky, S. H., and Rouse, D. I. 2003. Multiple disease resistance in interspecific hybrids of potato. Plant Dis. 87:266-272.
32. Jansky, S., Rouse, D. I., and Kauth, P. J. 2004. Inheritance of resistance to Verticillium dahliae in diploid interspecific potato hybrids. Plant Dis. 88:1075-1078.
33. Johansen, R. H., Farnsworth, B., Secor, G. A., Gudmestad, N. C, Thompson-Johns, A., and Holm, E. T 1994. Goldrush: a new high quality russet-skinned potato cultivar. Am. Potato J. 71:809-815.
34. Li, K. N., Rouse, D. I., Eyestone, E. J., and German, T L. 1999. The generation of specific DNA primers using random amplified polymorphic DNA and its application to Verticillium dahliae. Mycol. Res. 103:1361-1368.
35. Love, S. L., Novy, R., Corsini, D. L., Pavek, J. J., Mosley, A. R., Thornton, R. E., James, S. R., and Hane, D. C. 2002. Gem Russet: a long russet potato variety with excellent fresh market and French fry processing quality. Am. Potato! 79:25-31.
36. Love, S. L., Novy, R. G., Whitworth, J., Corsini, D. L., Pavek, J. J., Mosley, A. R., Pavek, M. J., Knowles, N. R., Brown, C. R., James, S. R., Hane, D. C, and Miller, J. C. 2006. GemStar Russet: a potato variety with high yield, good culinary quality, excellent fresh market appearance, and resistance to common scab. Am. Potato J. 83:171-180.
37. Love, S. L., Novy, R. G., Whitworth, J., Corsini, D. L., Pavek, J. J., Mosley, A. R., Thornton, R. E., Knowles, N. R., James, S. R., and Hane, D. C. 2005. Summit Russet: a new russet potato variety with good fresh market and frozen processing qualities. Am. J. Potato Res. 82:425-432.
38. MacRae, A., and Noling, J. 2010. Overview of new EPA regulations affecting use of metam sodium and metam potassium. Univ. Fla. Ext HS1167.
39. Mahuku, G. S., Platt, H. W, and Maxwell, P. 1999. Comparison of polymerase chain reaction based methods with plating on media to detect and identify Verticillium wilt pathogens of potato. Can. J. Plant Pathol. 21:125-131.
40. Mercado-Blanco, J., Rodriguez-Jurado, D., Parrilla-Araujo, S., and Jimenez-Diaz, R. M. 2003. Simultaneous detection of the defoliating and non-defoliating Verticillium dahliae pathotypes in infected olive plants by duplex, nested polymerase chain reaction. Plant Dis. 87:1487-1494.
41. Mercado-Blanco, J., Rodriguez-Jurado, D., Perez-Artes, E., and Jimenez-Diaz, R. M. 2001. Detection of the nondefoliating pathotype of Verticillium dahliae in infected olive plants by nested PCR. Plant Pathol. 50:1-12.
42. Mercado-Blanco, J., Rodriguez-Jurado, D., Perez-Artes, E., and Jimenez-Diaz, R. M. 2002. Detection of the defoliating pathotype of Verticillium dahliae in infected olive plants by nested PCR. Eur. J. Plant Pathol. 108:1-13.
43. Miller, P. M. 1955. V8 juice agar as a general purpose medium for fungi and bacteria. Phytopathology 45:460-462.
44. Mosley, A. R., James, S. R., Hane, D. C, Rykbost, K. A., Shock, C. C, Charlton, B. A., Pavek, J. J., Love, S. L., Corsini, D. L., and Thornton, R. E. 1999. Umatilla Russet: a full season long russet for processing and fresh market use. Am. J. Potato Res. 77:83-87.
45. Mosley, A. R., James, S. R., Rykbost, K. A., Hane, D. C, Stranger, C. E., Shock, C. C, Pavek, J. J., Corsini, D. L., Miller, J. C, Love, S. L., Holm, D. G., Thornton, R. E., and Voss, R. E. 2000. Century Russet: a high-yielding fresh market cultivar with Verticillium resistance. Am. Potato J. 77:161-165.
46. Mosley, A. R., Rykbost, K. A., James, S. R., Hane, D. C, Shock, C. C, Charlton, B. A., Pavek, J. J., Love, S. L., Corsini, D. L., and Thornton, R. E. 2001. Klamath Russet: a full season, fresh market, long russet. Am. J. Potato Res. 78:377-381.
47. Navarre, R., Shakya, R., Holden, J., and Crosslin, J. M. 2009. LC-MS Analysis of phenolic compounds in tubers showing Zebra Chip symptoms. Am. J. Potato Res. 86:88-95.
48. Nazar, R. N., Hu, X., Schmidt, J., Culham, D., and Robb, J. 1991. Potential use of PCR-amplified ribosomal intergenic sequences in the detection and differentiation of Verticillium wilt pathogens. Physiol. Mol. Plant Pathol. 39:1-11.
49. Nicot, P. C, and Rouse, D. I. 1987. Relationship between soil inoculums density of Verticillium dahliae and systemic colonization of potato stems in commercial fields over time. Phytopathology 77:1346-1355.
50. Nnodu, E. C, and Harrison, M. D. 1979. The relationship between Verticillium albo-atrum inoculum density and potato yield. Am. J. Potato Res. 56:11-25.
51. Novy, R. G., Corsini, D. L., Love, S. L., Pavek, J. J., Mosley, A. R., James, S. R., Hane, D. C, Shock, C. C, Rykbost, K. A., Brown, C. R., and Thornton, R. E. 2002. Bannock Russet: a dual-purpose, russet potato cultivar with high U.S. No. 1 yield and multiple disease resistances. Am. J. Potato Res. 79:147-153.
52. Novy, R. G., Corsini, D. L., Love, S. L., Pavek, J. J., Mosley, A. R., James, S. R., Hane, D. C., Shock, C. C., Rykbost, K. A., Brown, C. R., and Thornton, R. E. 2003. Alturas: a multi-purpose, russet potato cultivar with high yield and tuber specific gravity. Am. J. Potato Res. 80:295-301.
53. Novy, R. G., Love, S. L., Corsini, D. L., Pavek, J. J., Whitworth, J. L., Mosely, A. R., James, S. R., Hane, D. C., Shock, C. C., Rykbost, K. A., Brown, C. R., Thornton, R. E., Knowles, N. R., Pavek, M. J., Olsen, N., and Inglis, D. A. 2006. Defender: a high-yielding, processing potato cultivar with foliar and tuber resistance to late blight. Am. Potato J. 83:9-19.
54. Novy, R. G., Whitworth, J. L., Stark, J. C., Love, S. L., Corsini, D. L., Pavek, J. J., Vales, M. L., James, S. R., Hane, D. C, Shock, C. C, Charlton, B. A., Brown, C. R., Knowles, N. R., Pavek, M. J., Brandt, T L., Gupta, S., and Olsen, N. 2010. Clear water Russet: a dual purpose cultivar with cold sweetening resistance, high protein content, and low incidence of external defects and sugar ends. Am. Potato J. 87:458-471.
55. Novy, R. G., Whitworth, J. L., Stark, J. C, Love, S. L., Corsini, D. L., Pavek, J. J., Vales, M. I., James, S. R., Hane, D. C, Shock, C. C, Charlton, B. A., Brown, C. R., Knowles, N. R., Pavek, M. J., Brandt, T L., and Olsen, N. 2008. Premier Russet: a dual purpose, potato cultivar with significant resistance to low temperature sweetening during long-term storage. Am. J. Potato Res. 85:198-209.
56. Otazu, V, Gudmestad, N. C, and Zink, R. T, 1978. The role of Colleto-trichum atramentarium in the potato wilt complex in North Dakota. Plant Dis. Rep. 62:847-851.
57. Perez-Artes, E., Garcia-Pedrajas, M. D., Bejarano-Alcazar, J., and Jimenez-Diaz, R. M. 2000. Differentiation of cotton-defoliating and nondefoliating pathotypes of Verticillium dahliae by RAPD and specific PCR analyses. Eur. J. Plant Pathol. 106:507-517.
58. Powelson, M. L., Johnson, K. B., and Rowe, R. C. 1993. Management of diseases caused by soilborne pathogens. Pages 149-151 in: Potato Health Management. R. C. Rowe, ed. American Phytopathological Society, St. Paul, MN.
59. Powelson, M. L., and Rowe, R. C. 1993. Biology and management of early dying of potatoes. Annu. Rev. Phytopathol. 31:111-126.
60. Powelson, M. L., and Rowe, R. C. 1994. Potato early dying: causes and management tactics in the eastern and western United States. Pages 178-190 in: Advances in Potato Pest Biology and Management. G. W. Zehnder, M. L. Powelson, R. K. Jansson, and K. V Raman, eds. American Phytopathological Society, St. Paul, MN.
61. Robinson, D. B., and Ayers, G. W 1961. Verticillium wilt of potato in relation to vascular infection of the tuber. Can. J. Plant Sci. 41:703-708.
62. Rowe, R. C. 1985. Potato early dying-a serious threat to the potato industry. Am. Potato J. 62:157-161.
63. Rowe, R. C, and Powelson, M. L. 2002. Potato early dying: management challenges in a changing production environment. Plant Dis. 86:1184-1193.
64. Shaner, G., and Finney, R. E. 1977. The effect of nitrogen fertilization on the expression of slow-mildewing resistance in Knox wheat. Phytopathology 67:1051-1056.
65. Stark, J. C, Novy, R. G., Whitworth, J. L., Love, S. L., Corsini, D. L., Pavek, J. J., Vales, M. I., James, S. R., Hane, D. C, Charlton, B. A., Brown, C. R., Knowles, N. R., Pavek, M. J., and Brandt, T L. 2009. Highland Russet: a full season, processing variety with high yields of uniform U.S. no. 1 tubers. Am. Potato J. 86:171-182.
66. Taylor, R. J., Pasche, J. S., and Gudmestad, N. C. 2005. Influence of tillage and method of metam sodium application on distribution and survival of Verticillium dahliae in the soil and the development of Verticillium wilt of potato. Am. J. Potato Res. 82:451-461.
67. Tsompana, M., Abad, J., Purugganan, M., and Moyer, J. W. 2005. The molecular population genetics of the Tomato spotted wilt virus (TSWV) genome. Mol. Ecol. 14:53-66.
68. Westfall, P. H., Tobias, R. D., Rom, D., Wolfinger, R. D., and Hochberg, Y. 1999. Chapter 2: Concepts in basic methods for multiple comparisons and tests. Pages 13-40 in: Multiple Comparisons and Multiple Tests. SAS Institute, Inc., Cary, NC.
69. Wilhelm, S. 1955. Longevity of Verticillium wilt fungus in the laboratory and field. Phytopathology 45:180-181.
70. Xiao, C. L., and Subbarao, K. V 1998. Relationships between Verticillium dahliae inoculum density and wilt incidence, severity, and growth of cauliflower. Phytopathology 88:1108-1115.