A new StaRT-PCR approach to detect and quantify fish Viral Hemorrhagic Septicemia virus (VHSv): Enhanced quality control with internal standards

Journal of Virological Methods

Volumn 189, Issue 1, 2013, Pages 129-142

  Pierce, Lindsey R ^a   Willey, James C ^b   Crawford, Erin L ^b   Palsule, Vrushalee V ^a   Leaman, Douglas W ^a   Faisal, Mohamed ^c   Kim, Robert K ^c   Shepherd, Brian S ^d   Stanoszek, Lauren M ^b   Stepien, Carol A ^a  

^a UNIVERSITY OF TOLEDO   (United States)

^b MEDICAL COLLEGE OF OHIO   (United States)

^c MICHIGAN STATE UNIVERSITY   (United States)

^d UNIVERSITY OF WISCONSIN MILWAUKEE   (United States)

Author keywords

Cell culture; Diagnostic validation; Fish virus; Real time PCR; StaRT PCR; Viral Hemorrhagic Septicemia virus (VHSv)

Indexed keywords

EDETIC ACID; RNA;

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; ASIA; CONTROLLED STUDY; DIAGNOSTIC ACCURACY; EUROPE; FALSE NEGATIVE RESULT; FISH DISEASE; IMMERSION; INJECTION; NONHUMAN; NORTH AMERICA; PRIORITY JOURNAL; QUALITY CONTROL; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE; SENSITIVITY AND SPECIFICITY; SKIN DEFECT; STANDARD; STANDARDIZED REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; VIRAL HEMORRHAGIC SEPTICEMIA VIRUS; VIRUS DETECTION; VIRUS STRAIN;

ANIMALS; BASE SEQUENCE; CELL LINE; ESOCIDAE; FALSE NEGATIVE REACTIONS; FISH DISEASES; MOLECULAR SEQUENCE DATA; NOVIRHABDOVIRUS; ORGANIC CHEMICALS; PERCHES; QUALITY CONTROL; REFERENCE STANDARDS; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RHABDOVIRIDAE INFECTIONS;

EID: 84875254593     PISSN: 01660934     EISSN: 18790984     Source Type: Journal    
DOI: 10.1016/j.jviromet.2013.01.006     Document Type: Article

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