Development and validation of a novel Taqman-based real-time RT-PCR assay suitable for demonstrating freedom from viral haemorrhagic septicaemia virus

Journal of Fish Diseases

Volumn 36, Issue 1, 2013, Pages 9-23

  Jonstrup, S P ^a   Kahns, S ^a   Skall, H F ^a   Boutrup, T S ^a   Olesen, N J ^a  

^a TECHNICAL UNIVERSITY OF DENMARK   (Denmark)

Author keywords

Real time RT PCR; Validation; Viral haemorrhagic septicaemia; Viral haemorrhagic septicaemia virus

Indexed keywords

PRIMER DNA; VIRUS RNA;

ANIMAL; ANIMAL DISEASE; ARTICLE; ENZYME LINKED IMMUNOSORBENT ASSAY; FISH DISEASE; FISHERY; GENETICS; METHODOLOGY; REAL TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY; RHABDOVIRUS INFECTION; SENSITIVITY AND SPECIFICITY; STANDARD; VALIDATION STUDY; VIRAL HEMORRHAGIC SEPTICEMIA VIRUS;

ANIMALS; DNA PRIMERS; ENZYME-LINKED IMMUNOSORBENT ASSAY; FISH DISEASES; FISHERIES; NOVIRHABDOVIRUS; REAL-TIME POLYMERASE CHAIN REACTION; REPRODUCIBILITY OF RESULTS; RHABDOVIRIDAE INFECTIONS; RNA, VIRAL; SENSITIVITY AND SPECIFICITY;

RHABDOVIRIDAE; VIRAL HEMORRHAGIC SEPTICEMIA VIRUS;

EID: 84871286199     PISSN: 01407775     EISSN: 13652761     Source Type: Journal    
DOI: 10.1111/j.1365-2761.2012.01416.x     Document Type: Article

References (65)

1. Benmansour A., Basurco B., Monnier A.F., Vende P., Winton J.R. & de Kinkelin P. (1997) Sequence variation of the glycoprotein gene identifies three distinct lineages within field isolates of viral haemorrhagic septicaemia virus, a fish rhabdovirus. Journal of General Virology 78, 2837-2846.
2. Bernard J., Bremont M. & Winton J. (1992) Nucleocapsid gene sequence of a North American isolate of viral haemorrhagic septicaemia virus, a fish rhabdovirus. Journal of General Virology 73, 1011-1014.
3. Brunson R., True K. & Yancey J. (1989) VHS virus isolated at Makah national fish hatchery. American Fisheries Society Fish Health Section Newsletter 17, 3.
4. Buh G.M., Tengs T., La Paz J.L., Holst-Jensen A., Pla M., Esteve T., Zel J. & Gruden K. (2010) Comparison of nine different real-time PCR chemistries for qualitative and quantitative applications in GMO detection. Analytical and Bioanalytical Chemistry 396, 2023-2029.
5. Castric J., Jeffroy J., Bearzotti M. & de Kinkelin P. (1992) Isolation of viral haemorrhagic septicaemia virus (VHSV) from wild elvers Anguilla anguilla. Bulletin of the European Association of Fish Pathologists 12, 21-23.
6. Chico V., Gomez N., Estepa A. & Perez L. (2006) Rapid detection and quantitation of viral hemorrhagic septicemia virus in experimentally challenged rainbow trout by real-time RT-PCR. Journal of Virological Methods 132, 154-159.
7. Cutrín J.M., Olveira J.G., Bandín I. & Dopazo C.P. (2009) Validation of real time RT-PCR applied to cell culture for diagnosis of any known genotype of viral haemorrhagic septicaemia virus. Journal of Virological Methods 162, 155-162.
8. Dale O.B., Ørpetveit I., Lyngstad T.M., Kahns S., Skall H.F., Olesen N.J. & Dannevig B.H. (2009) Outbreak of viral haemorrhagic septicaemia (VHS) in seawater-farmed rainbow trout in Norway caused by VHS virus Genotype III. Diseases of Aquatic Organisms 85, 93-103.
9. Dixon P.F., Feist S., Kehoe E., Parry L., Stone D.M. & Way K. (1997) Isolation of viral haemorrhagic septicaemia virus from Atlantic herring Clupea harengus from the English Channel. Diseases of Aquatic Organisms 30, 81-89.
10. Dopazo C.P., Bandín I., López-Vazquez C., Lamas J., Noya M. & Barja J.L. (2002) Isolation of viral hemorrhagic septicemia virus from Greenland halibut Reinhardtius hippoglossoides caught at the Flemish Cap. Diseases of Aquatic Organisms 50, 171-179.
11. Drake J.W. & Holland J.J. (1999) Mutation rates among RNA viruses. Proceedings of the National Academy of Science of the United States of America 96, 13910-13913.
12. Einer-Jensen K., Ahrens P., Forsberg R. & Lorenzen N. (2004) Evolution of the fish rhabdovirus viral haemorrhagic septicaemia virus. Journal of General Virology 85, 1167-1179.
13. Elsayed E., Faisal M., Thomas M., Whelan G., Batts W. & Winton J. (2006) Isolation of viral haemorrhagic septicaemia virus from muskellunge, Esox masquinongy (Mitchill), in Lake St Clair, Michigan, USA reveals a new sublineage of the North American genotype. Journal of Fish Diseases 29, 611-619.
14. Emmenegger E.J., Kentop E., Thompson T.M., Pittam S., Ryan A., Keon D., Carlino J.A., Ranson J., Life R.B., Troyer R.M., Garver K.A. & Kurath G. (2011) Development of an aquatic pathogen database (AquaPathogen X) and its utilization in tracking emerging fish virus pathogens in North America. Journal of Fish Diseases 34, 579-587.
15. EU Commission (2001) 2001/183/EC: Commission Decision of 22 February 2001 laying down the sampling plans and diagnostic methods for the detection and confirmation of certain fish diseases and repealing Decision 92/532/EEC (Text with EEA relevance) (notified under document number C(2001) 426). Official Journal of the European Communities L 67, 65-76.
16. Gagné N., Mackinnon A.M., Boston L., Souter B., Cook-Versloot M., Griffiths S. & Olivier G. (2007) Isolation of viral haemorrhagic septicaemia virus from mummichog, stickleback, striped bass and brown trout in eastern Canada. Journal of Fish Diseases 30, 213-223.
17. Garver K.A., Hawley L.M., McClure C.A., Schroeder T., Aldous S., Doig F., Snow M., Edes S., Baynes C. & Richard J. (2011) Development and validation of a reverse transcription quantitative PCR for universal detection of viral hemorrhagic septicemia virus. Diseases of Aquatic Organisms 95, 97-112.
18. Groocock G.H., Getchell R.G., Wooster G.A., Britt K.L., Batts W.N., Winton J.R., Casey R.N., Casey J.W. & Bowser P.R. (2007) Detection of viral hemorrhagic septicemia in round gobies in New York State (USA) waters of Lake Ontario and the St. Lawrence River. Diseases of Aquatic Organisms 76, 187-192.
19. Guillou J.P., Merle G., Henault S. & Hattenberger A.M. (1999) Detection of viral hemorrhagic septicemia virus (VHSV) in rainbow trout (Oncorhynchus mykiss) by reverse transcription followed by polymerase chain reaction. Diagnostic validation. Veterinary Research 30, 49-60.
20. Håstein T., Holt G. & Krogsrud J. (1968) Hemorrhagisk virusseptikemi (Egtvedsyke) hos regnbueørret i Norge. Nordisk Veterinærmedicin 20, 708-711.
21. Hedrick R.P., Batts W.N., Yun S., Traxler G.S., Kaufman J. & Winton J.R. (2003) Host and geographic range extensions of the North American strain of viral hemorrhagic septicemia virus. Diseases of Aquatic Organisms 55, 211-220.
22. Heid C.A., Stevens J., Livak K.J. & Williams P.M. (1996) Real time quantitative PCR. Genome Research 6, 986-994.
23. Hope K.M., Casey R.N., Groocock G.H., Getchell R.G., Bowser P.R. & Casey J.W. (2010) Comparison of quantitative RT-PCR with cell culture to detect viral hemorrhagic septicemia virus (VHSV) IVb infections in the Great Lakes. Journal of Aquatic Animal Health 22, 50-61.
24. Hopper K. (1989) The isolation of vhsv from chinook salmon at Glenwood Springs, Orcas Island, Washington. American Fisheries Society Fish Health Section Newsletter 17, 1.
25. Ito T., Olesen N.J., Skall H.F., Sano M., Kurita J., Nakajima K. & Iida T. (2010) Development of a monoclonal antibody against viral haemorrhagic septicaemia virus (VHSV) genotype IVa. Diseases of Aquatic Organisms 89, 17-27.
26. Jensen M.H. (1963) Preparation of fish tissue cultures for virus research. Bulletin Office International Epizooties 59, 131-134.
27. Jensen M.H. (1965) Research on the virus of Egtved disease. Annals of the New York Academy of Sciences 126, 422-426.
28. Jensen N.J., Bloch B. & Larsen J.L. (1979) The ulcus-syndrome in cod (Gadus morhua). III. A preliminary virological report. Nordisk Veterinærmedicin 31, 436-442.
29. Jonstrup S.P., Gray T., Kahns S., Skall H.F., Snow M. & Olesen N.J. (2009) FishPathogens.eu/vhsv: a user-friendly viral haemorrhagic septicaemia virus isolate and sequence database. Journal of Fish Diseases 32, 925-929.
30. Jonstrup S.P., Schütze H., Kurath G., Gray T., Jensen B.B. & Olesen N.J. (2010) An isolate and sequence database of infectious haematopoietic necrosis virus (IHNV). Journal of Fish Diseases 33, 469-471.
31. Jørgensen P.E.V., Einer-Jensen K., Higman K.H. & Winton J.R. (1995) Sequence comparison of the central region of the glycoprotein gene of neutralizable, non-neutralizable, and serially passed isolates of viral haemorrhagic septicaemia virus. Diseases of Aquatic Organisms 23, 77-82.
32. Kahns S., Skall H.F., Kaas R.S., Korsholm H., Bang Jensen B., Jonstrup S.P., Dodge M.J., Einer-Jensen K., Stone D. & Olesen N.J. (2012) European freshwater VHSV genotype Ia isolates divides into two distinct subpopulations. Diseases of Aquatic Organisms 99, 23-35.
33. de Kinkelin P. & Le Berre M. (1977) Isolement d'un Rhabdovirus pathogéne de la Truite Fario (Salmo trutta L., 1766). Comptes Rendus Academic Science 284, 101-104.
34. Knüsel R., Bergmann S.M., Einer-Jensen K., Casey J., Segner H. & Wahli T. (2007) Virus isolation vs RT-PCR: which method is more successful in detecting VHSV and IHNV in fish tissue sampled under field conditions? Journal of Fish Diseases 30, 559-568.
35. Le Berre M., de Kinkelin P. & Metzger A. (1977) Identification sérologique des Rhabdovirus des Salmonidés. Bulletin Office International Epizooties 87, 391-393.
36. Liu Z., Teng Y., Liu H., Jiang Y., Xie X., Li H., Lv J., Gao L., He J., Shi X., Tian F., Yang J. & Xie C. (2008) Simultaneous detection of three fish rhabdoviruses using multiplex real-time quantitative RT-PCR assay. Journal of Virological Methods 149, 103-109.
37. Lorenzen N., Olesen N.J. & Jørgensen P.E.V. (1988) Production and characterization of monoclonal antibodies to four Egtved virus structural proteins. Diseases of Aquatic Organisms 4, 35-42.
38. Lorenzen N., Olesen N.J., Vestergård Jørgensen P.E., Etzerodt M., Holtet T.L. & Thøgersen H.C. (1993) Molecular cloning and expression in Escherichia coli of the glycoprotein gene of VHS virus, and immunization of rainbow trout with the recombinant protein. Journal of General Virology 74, 623-630.
39. Lorenzen E., Carstensen B. & Olesen N.J. (1999) Inter-laboratory comparison of cell lines for susceptibility to three viruses: VHSV, IHNV and IPNV. Diseases of Aquatic Organisms 37, 81-88.
40. Mackay I.M. (2004) Real-time PCR in the microbiology laboratory. Clinical Microbiology and Infection 10, 190-212.
41. Matejusova I., McKay P., McBeath A.J., Collet B. & Snow M. (2008) Development of a sensitive and controlled real-time RT-PCR assay for viral haemorrhagic septicaemia virus (VHSV) in marine salmonid aquaculture. Diseases of Aquatic Organisms 80, 137-144.
42. Meyers T.R. & Winton J.R. (1995) Viral hemorrhagic septicemia virus in North America. Annual Review of Fish Diseases 5, 3-24.
43. Miller T.A., Rapp J., Wastlhuber U., Hoffmann R.W. & Enzmann P.J. (1998) Rapid and sensitive reverse transcriptase-polymerase chain reaction based detection and differential diagnosis of fish pathogenic rhabdoviruses in organ samples and cultured cells. Diseases of Aquatic Organisms 34, 13-20.
44. Mortensen H.F., Heuer O.E., Lorenzen N., Otte L. & Olesen N.J. (1999) Isolation of viral haemorrhagic septicaemia virus (VHSV) from wild marine fish species in the Baltic Sea, Kattegat, Skagerrak and the North Sea. Virus Research 63, 95-106.
45. Nishizawa T., Iida H., Takano R., Isshiki T., Nakajima K. & Muroga K. (2002) Genetic relatedness among Japanese, American and European isolates of viral hemorrhagic septicemia virus (VHSV) based on partial G and P genes. Diseases of Aquatic Organisms 48, 143-148.
46. Nordblom B. (1998) Report on an Outbreak of Viral Haemorrhagic Septicaemia in Sweden. Report for the Standing Veterinary Committee, Swedish Board of Agriculture, Department for Animal Production and Health.
47. Nordblom B. & Norell A.W. (2000) Report on an Outbreak of VHS (Viral Hemorrhagic Septicaemia) in Farmed Fish in Sweden. Report for the Standing Veterinary Committee, Swedish Board of Agriculture, Department for Animal Production and Health.
48. Olesen N.J., Lorenzen N. & Jørgensen P.E.V. (1991) Detection of rainbow trout antibody to Egtved virus by enzym-linked immunosorbent assay (ELISA), immunofluorescence (IF), and plaque neutralization tests (50%PNT). Diseases of Aquatic Organisms 10, 31-38.
49. Orlando C., Pinzani P. & Pazzagli M. (1998) Developments in quantitative PCR. Clinical Chemistry and Laboratory Medicine 36, 255-269.
50. Raja-Halli M., Vehmas T.K., Rimaila-Parnanen E., Sainmaa S., Skall H.F., Olesen N.J. & Tapiovaara H. (2006) Viral haemorrhagic septicaemia (VHS) outbreaks in Finnish rainbow trout farms. Diseases of Aquatic Organisms 72, 201-211.
51. Rose J. & Schubert M. (1987) Rhabdovirus genomes and their products. In: The Rhabdoviruses (ed. by R.R. Wagner), pp. 129-166. Plenum Press, New York.
52. Ross K., McCarthy U., Smail D.A., Wood P., Stuart D., Rough E.I. & Bruno W. (1994) An outbreak of viral haemorrhagic septicaemia (VHS) in turbot (Scophthalmus maximus) in Scotland. Bulletin of the European Association of Fish Pathologists 14, 213-214.
53. Rovozzo G.C. & Burke C.N. (1973) A Manual of Basic Virological Techniques. Prentice Hall, Englewood Cliffs, NJ.
54. Schlotfeldt H.J., Ahne W., Jørgensen P.E.V. & Glende W. (1991) Occurrence of viral haemorrhagic septicaemia in turbot (Scophthalmus maximus) - a natural outbreak. Bulletin of the European Association of Fish Pathologists 11, 105-107.
55. Schütze H., Mundt E. & Mettenleiter T.C. (1999) Complete genomic sequence of viral hemorrhagic septicemia virus, a fish rhabdovirus. Virus Genes 19, 59-65.
56. Skall H.F., Olesen N.J. & Mellergaard S. (2005) Viral haemorrhagic septicaemia virus in marine fish and its implications for fish farming-a review. Journal of Fish Diseases 28, 509-529.
57. Smail D.A. (2000) Isolation and identification of viral haemorrhagic septicaemia (VHS) viruses from cod Gadus morhua with the ulcus syndrome and from haddock Melanogrammus aeglefinus having skin haemorrhages in the North Sea. Diseases of Aquatic Organisms 41, 231-235.
58. Snow M., Cunningham C.O., Melvin W.T. & Kurath G. (1999) Analysis of the nucleoprotein gene identifies distinct lineages of viral haemorrhagic septicaemia virus within the European marine environment. Virus Research 63, 35-44.
59. Snow M., Bain N., Black J., Taupin V., Cunningham C.O., King J.A., Skall H.F. & Raynard R.S. (2004) Genetic population structure of marine viral haemorrhagic septicaemia virus (VHSV). Diseases of Aquatic Organisms 61, 11-21.
60. Snow M., McKay P., McBeath A.J., Black J., Doig F., Kerr R., Cunningham C.O., Nylund A. & Devold M. (2006) Development, application and validation of a Taqman real-time RT-PCR assay for the detection of infectious salmon anaemia virus (ISAV) in Atlantic salmon (Salmo salar). Developmental Biology 126, 133-145.
61. Takano R., Nishizawa T., Arimoto M. & Muroga K. (2000) Isolation of viral haemorrhagic septicaemia virus (VHSV) from wild Japanese flounder, Paralichthys olivaceus. Bulletin of the European Association of Fish Pathologists 20, 186-192.
62. Traxler G., Kieser D. & Evelyn T.P.T. (1995) Isolation of North American strain of VHS virus from farmed Atlantic salmon. In: Aquaculture Update No. 72 (ed. by L. Margolis). pp. 1-2. Aquaculture Divison, Pacific Biological Station, Nanaimo, BC.
63. Vestergard Jorgensen P.E. & Olesen N.J. (1987) Cod ulcus syndrome rhabdovirus is indistinguishable from the Egtved (VHS) virus. Bulletin of the European Association of Fish Pathologists 7, 73-74.
64. Vestergård Jørgensen P.E. (1974) A study of viral diseases in Danish rainbow trout. Their diagnosis and control. Thesis from the Royal Veterinary- and Agricultural University, Denmark. pp. 1-101.
65. Williams K., Blake S., Sweeney A., Singer J.T. & Nicholson B.L. (1999) Multiplex reverse transcriptase PCR assay for simultaneous detection of three fish viruses. Journal of Clinical Microbiology 37, 4139-4141.