RNA helicase A is not required for RISC activity

Biochimica et Biophysica Acta - Gene Regulatory Mechanisms

Volumn 1829, Issue 10, 2013, Pages 1092-1101

  Liang, Xue hai ^a   Crooke, Stanley T ^a  

^a ISIS PHARMACEUTICALS   (United States)

Author keywords

Ago2; ASOs; Competition; MiRNA; RHA; RISC; SiRNA

Indexed keywords

ANTISENSE OLIGONUCLEOTIDE; MESSENGER RNA; MICRORNA; RNA HELICASE; RNA HELICASE A; RNA INDUCED SILENCING COMPLEX; SMALL INTERFERING RNA; UNCLASSIFIED DRUG;

ARTICLE; BIOLOGICAL ACTIVITY; CONTROLLED STUDY; FEMALE; HELA CELL; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DEPLETION; PROTEIN EXPRESSION; PROTEIN FUNCTION; PROTEIN INTERACTION; REVERSE TRANSCRIPTION POLYMERASE CHAIN REACTION; RNA INTERFERENCE;

AGO2; ANTISENSE OLIGONUCLEOTIDES; ASOS; COMPETITION; MIRNA; RHA; RISC; RNA HELICASE A; RNA INDUCED SILENCING COMPLEX; SIRNA;

BLOTTING, WESTERN; HELA CELLS; HUMANS; IMMUNOPRECIPITATION; MICRORNAS; OLIGONUCLEOTIDES, ANTISENSE; PHOSPHOPROTEINS; REAL-TIME POLYMERASE CHAIN REACTION; REVERSE TRANSCRIPTASE POLYMERASE CHAIN REACTION; RNA HELICASES; RNA, MESSENGER; RNA, SMALL INTERFERING; RNA-BINDING PROTEINS; RNA-INDUCED SILENCING COMPLEX;

EID: 84882687109     PISSN: 18749399     EISSN: 18764320     Source Type: Journal    
DOI: 10.1016/j.bbagrm.2013.07.008     Document Type: Article

References (27)

1. Fire A., Xu S., Montgomery M.K., Kostas S.A., Driver S.E., Mello C.C. Potent and specific genetic interference by double-stranded RNA in Caenorhabditis elegans. Nature 1998, 391:806-811.
2. Agrawal N., Dasaradhi P.V., Mohmmed A., Malhotra P., Bhatnagar R.K., Mukherjee S.K. RNA interference: biology, mechanism, and applications. Microbiol. Mol. Biol. Rev. 2003, 67:657-685.
3. Ghildiyal M., Zamore P.D. Small silencing RNAs: an expanding universe. Nat. Rev. Genet. 2009, 10:94-108.
4. Chekulaeva M., Filipowicz W. Mechanisms of miRNA-mediated post-transcriptional regulation in animal cells. Curr. Opin. Cell Biol. 2009, 21:452-460.
5. Koller E., Propp S., Murray H., Lima W., Bhat B., Prakash T.P., Allerson C.R., Swayze E.E., Marcusson E.G., Dean N.M. Competition for RISC binding predicts in vitro potency of siRNA. Nucleic Acids Res. 2006, 34:4467-4476.
6. Vickers T.A., Lima W.F., Nichols J.G., Crooke S.T. Reduced levels of Ago2 expression result in increased siRNA competition in mammalian cells. Nucleic Acids Res. 2007, 35:6598-6610.
7. Khan A.A., Betel D., Miller M.L., Sander C., Leslie C.S., Marks D.S. Transfection of small RNAs globally perturbs gene regulation by endogenous microRNAs. Nat. Biotechnol. 2009, 27:549-555.
8. Grimm D., Streetz K.L., Jopling C.L., Storm T.A., Pandey K., Davis C.R., Marion P., Salazar F., Kay M.A. Fatality in mice due to oversaturation of cellular microRNA/short hairpin RNA pathways. Nature 2006, 441:537-541.
9. Pan Q., de Ruiter P.E., von Eije K.J., Smits R., Kwekkeboom J., Tilanus H.W., Berkhout B., Janssen H.L., van der Laan L.J. Disturbance of the microRNA pathway by commonly used lentiviral shRNA libraries limits the application for screening host factors involved in hepatitis C virus infection. FEBS Lett. 2011, 585:1025-1030.
10. Grimm D., Wang L., Lee J.S., Schurmann N., Gu S., Borner K., Storm T.A., Kay M.A. Argonaute proteins are key determinants of RNAi efficacy, toxicity, and persistence in the adult mouse liver. J. Clin. Invest. 2010, 120:3106-3119.
11. Kai Z.S., Pasquinelli A.E. MicroRNA assassins: factors that regulate the disappearance of miRNAs. Nat. Struct. Mol. Biol. 2010, 17:5-10.
12. Liang X.H., Hart C.E., Crooke S.T. Transfection of siRNAs can alter miRNA levels and trigger non-specific protein degradation in mammalian cells. Biochim. Biophys. Acta 2013, 1829:455-468.
13. Meister G., Landthaler M., Peters L., Chen P.Y., Urlaub H., Luhrmann R., Tuschl T. Identification of novel argonaute-associated proteins. Curr. Biol. 2005, 15:2149-2155.
14. Chendrimada T.P., Finn K.J., Ji X., Baillat D., Gregory R.I., Liebhaber S.A., Pasquinelli A.E., Shiekhattar R. MicroRNA silencing through RISC recruitment of eIF6. Nature 2007, 447:823-828.
15. Liu Y., Ye X., Jiang F., Liang C., Chen D., Peng J., Kinch L.N., Grishin N.V., Liu Q. C3PO, an endoribonuclease that promotes RNAi by facilitating RISC activation. Science 2009, 325:750-753.
16. Robb G.B., Rana T.M. RNA helicase A interacts with RISC in human cells and functions in RISC loading. Mol. Cell 2007, 26:523-537.
17. Lee C.G., Hurwitz J. A new RNA helicase isolated from HeLa cells that catalytically translocates in the 3' to 5' direction. J. Biol. Chem. 1992, 267:4398-4407.
18. Zhong X., Safa A.R. RNA helicase A in the MEF1 transcription factor complex up-regulates the MDR1 gene in multidrug-resistant cancer cells. J. Biol. Chem. 2004, 279:17134-17141.
19. Tetsuka T., Uranishi H., Sanda T., Asamitsu K., Yang J.P., Wong-Staal F., Okamoto T. RNA helicase A interacts with nuclear factor kappaB p65 and functions as a transcriptional coactivator. Eur. J. Biochem. 2004, 271:3741-3751.
20. Pellizzoni L., Charroux B., Rappsilber J., Mann M., Dreyfuss G. A functional interaction between the survival motor neuron complex and RNA polymerase II. J. Cell Biol. 2001, 152:75-85.
21. Vickers T.A., Lima W.F., Wu H., Nichols J.G., Linsley P.S., Crooke S.T. Off-target and a portion of target-specific siRNA mediated mRNA degradation is Ago2 'Slicer' independent and can be mediated by Ago1. Nucleic Acids Res. 2009, 37:6927-6941.
22. Liang X.H., Crooke S.T. Depletion of key protein components of the RISC pathway impairs pre-ribosomal RNA processing. Nucleic Acids Res. 2011, 39:4875-4889.
23. Ginisty H., Sicard H., Roger B., Bouvet P. Structure and functions of nucleolin. J. Cell Sci. 1999, 112(Pt 6):761-772.
24. Loinger A., Shemla Y., Simon I., Margalit H., Biham O. Competition between small RNAs: a quantitative view. Biophys. J. 2012, 102:1712-1721.
25. Tomari Y., Du T., Haley B., Schwarz D.S., Bennett R., Cook H.A., Koppetsch B.S., Theurkauf W.E., Zamore P.D. RISC assembly defects in the Drosophila RNAi mutant armitage. Cell 2004, 116:831-841.
26. Geary R.S., Watanabe T.A., Truong L., Freier S., Lesnik E.A., Sioufi N.B., Sasmor H., Manoharan M., Levin A.A. Pharmacokinetic properties of 2'-O-(2-methoxyethyl)-modified oligonucleotide analogs in rats. J. Pharmacol. Exp. Ther. 2001, 296:890-897.
27. Tschuch C., Schulz A., Pscherer A., Werft W., Benner A., Hotz-Wagenblatt A., Barrionuevo L.S., Lichter P., Mertens D. Off-target effects of siRNA specific for GFP. BMC Mol. Biol. 2008, 9:60.