Human chorionic gonadotropin (hCG) in the secretome of cultured embryos: Hyperglycosylated hCG and hCG-free beta subunit are potential markers for infertility management and treatment

Reproductive Sciences

Volumn 20, Issue 9, 2013, Pages 1038-1045

  Butler, Stephen A ^a   Luttoo, Jameel ^a   Freire, Maísa O T ^b   Abban, Thomas K ^a   Borrelli, Paola T A ^c   Iles, Ray K ^d  

^a MIDDLESEX UNIVERSITY   (United Kingdom)

^b WHITTINGTON HOSPITAL   (United Kingdom)

^c BEDFORD HOSPITAL NHS TRUST   (United Kingdom)

^d ELK Foundation for Health Research   (United Kingdom)

Author keywords

embryo secretome; hCG; hCG ; hCGh; ICSI; implantation; IVF; marker

Indexed keywords

BIOLOGICAL MARKER; CHORIONIC GONADOTROPIN; CHORIONIC GONADOTROPIN BETA SUBUNIT; HYPERGLYCOSYLATED HUMAN CHORIONIC GONADOTROPIN; UNCLASSIFIED DRUG;

ARTICLE; BLASTOCYST; CLINICAL ARTICLE; CONTROLLED STUDY; CULTURE MEDIUM; EMBRYO; EMBRYO CELL; EMBRYO CULTURE; EMBRYO DEVELOPMENT; EMBRYO TRANSFER; EMBRYO VIABILITY; ENZYME LINKED IMMUNOSORBENT ASSAY; FERTILIZATION IN VITRO; GENE EXPRESSION; HATCHING; HUMAN; HUMAN CELL; IMMUNOREACTIVITY; INFERTILITY; INFERTILITY THERAPY; MATRIX ASSISTED LASER DESORPTION IONIZATION TIME OF FLIGHT MASS SPECTROMETRY; MORULA; PRIORITY JOURNAL;

EMBRYO SECRETOME; HCG; HCGΒ; HCGH; ICSI; IMPLANTATION; IVF; MARKER;

BIOLOGICAL MARKERS; BLASTOCYST; CHORIONIC GONADOTROPIN; CHORIONIC GONADOTROPIN, BETA SUBUNIT, HUMAN; CULTURE MEDIA, CONDITIONED; EMBRYO CULTURE TECHNIQUES; EMBRYO IMPLANTATION; EMBRYO TRANSFER; FEMALE; FERTILIZATION IN VITRO; GLYCOSYLATION; HUMANS; INFERTILITY; PREGNANCY; PROGNOSIS; SPECTROMETRY, MASS, MATRIX-ASSISTED LASER DESORPTION-IONIZATION;

EID: 84882333860     PISSN: 19337191     EISSN: 19337205     Source Type: Journal    
DOI: 10.1177/1933719112472739     Document Type: Article

References (45)

1. Cole LA, Butler S. Detection of hCG in trophoblastic disease. the USA hCG reference service experience. J Reprod Med. 2002 ; 47 (6). 433-444
2. Cole LA, Dai D, Butler SA, Leslie KK, Kohorn EI. Gestational trophoblastic diseases: 1. pathophysiology of hyperglycosylated hCG. Gynecol Oncol. 2006 ; 102 (2). 145-150
3. Birken S, Krichevsky A, O'Connor J, et al. Development and characterization of antibodies to a nicked and hyperglycosylated form of hCG from a choriocarcinoma patient: Generation of antibodies that differentiate between pregnancy hCG and choriocarcinoma hCG. Endocrine. 1999 ; 10 (2). 137-144
4. Butler SA, Khanlian SA, Cole LA. Detection of early pregnancy forms of human chorionic gonadotropin by home pregnancy test devices. Clin Chem. 2001 ; 47 (12). 2131-2136
5. Cole LA. New discoveries on the biology and detection of human chorionic gonadotropin. Reprod Biol Endocrinol. 2009 ; 7: 8
6. Kovalevskaya G, Birken S, Kakuma T, O'Connor JF. Early pregnancy human chorionic gonadotropin (hCG) isoforms measured by an immunometric assay for choriocarcinoma-like hCG. J Endocrinol. 1999 ; 161 (1). 99-106
7. Kovalevskaya G, Genbacev O, Fisher SJ, Caceres E, O'Connor JF. Trophoblast origin of hCG isoforms: Cytotrophoblasts are the primary source of choriocarcinoma-like hCG. Mol Cell Endocrinol. 2002 ; 194 (1-2). 147-155
8. Kovalevskaya G, Kakuma T, Schlatterer J, O'Connor JF. Hyperglycosylated HCG expression in pregnancy: Cellular origin and clinical applications. Mol Cell Endocrinol. 2007 ;: 260 237-262 243
9. Mock P, Kovalevskaya G, O'Connor JF, Campana A. Choriocarcinoma-like human chorionic gonadotrophin (HCG) and HCG bioactivity during the first trimester of pregnancy. Hum Reprod. 2000 ; 15 (10). 2209-2214
10. Elliott MM, Kardana A, Lustbader JW, Cole LA. Carbohydrate and peptide structure of the alpha- and beta-subunits of human chorionic gonadotropin from normal and aberrant pregnancy and choriocarcinoma. Endocrine. 1997 ; 7 (1). 15-32
11. Dimitriadou F, Phocas I, Mantzavinos T, Sarandakou A, Rizos D, Zourlas PA. Discordant secretion of pregnancy specific beta 1-glycoprotein and human chorionic gonadotropin by human pre-embryos cultured in vitro. Fertil Steril. 1992 ; 57 (3). 631-636
12. Dokras A, Sargent IL, Ross C, Gardner RL, Barlow DH. The human blastocyst: Morphology and human chorionic gonadotrophin secretion in vitro. Hum Reprod. 1991 ; 6 (8). 1143-1151
13. Hay DL, Lopata A. Chorionic gonadotropin secretion by human embryos in vitro. J Clin Endocrinol Metab. 1988 ; 67 (6). 1322-1324
14. Lopata A, Hay DL. The potential of early human embryos to form blastocysts, hatch from their zona and secrete HCG in culture. Hum Reprod. 1989 ; 4 (suppl 8). 87-94
15. Lopata A, Hay DL. The surplus human embryo: Its potential for growth, blastulation, hatching, and human chorionic gonadotropin production in culture. Fertil Steril. 1989 ; 51 (6). 984-991
16. Lopata A, Oliva K. Chorionic gonadotrophin secretion by human blastocysts. Hum Reprod. 1993 ; 8 (6). 932-938
17. Lopata A, Oliva K, Stanton PG, Robertson DM. Analysis of chorionic gonadotrophin secreted by cultured human blastocysts. Mol Hum Reprod. 1997 ; 3 (6). 517-521
18. Turner K, Lenton EA. The influence of vero cell culture on human embryo development and chorionic gonadotrophin production in vitro. Hum Reprod. 1996 ; 11 (9). 1966-1974
19. Woodward BJ, Lenton EA, Turner K.. Human chorionic gonadotrophin: Embryonic secretion is a time-dependent phenomenon. Hum Reprod. 1993 ; 8 (9). 1463-1468
20. Jurisicova A, Antenos M, Kapasi K, Meriano J, Casper RF. Variability in the expression of trophectodermal markers beta-human chorionic gonadotrophin, human leukocyte antigen-G and pregnancy specific beta-1 glycoprotein by the human blastocyst. Hum Reprod. 1999 ; 14 (7). 1852-1858
21. Kovalevskaya G, Birken S, Kakuma T, et al. Differential expression of human chorionic gonadotropin (hCG) glycosylation isoforms in failing and continuing pregnancies: Preliminary characterization of the hyperglycosylated hCG epitope. J Endocrinol. 2002 ; 172 (3). 497-506
22. O'Connor JF, Ellish N, Kakuma T, Schlatterer J, Kovalevskaya G. Differential urinary gonadotrophin profiles in early pregnancy and early pregnancy loss. Prenat Diagn. 1998 ; 18 (12). 1232-1240
23. Sutton-Riley JM, Khanlian SA, Byrn FW, Cole LA. A single serum test for measuring early pregnancy outcome with high predictive value. Clin Biochem. 2006 ; 39 (7). 682-687
24. Sasaki Y, Ladner DG, Cole LA. Hyperglycosylated human chorionic gonadotropin and the source of pregnancy failures. Fertil Steril. 2008 ; 89 (6). 1781-1786
25. Cole LA. Hyperglycosylated hCG and pregnancy failures. J Reprod Immunol. 2012 ; 93 (2). 119-122
26. Rienzi L, Ubaldi F, Iacobelli M, et al. Significance of morphological attributes of the early embryo. Reprod Biomed Online. 2005 ; 10 (5). 669-681
27. Jones GM, Cram DS, Song B, Kokkali G, Pantos K, Trounson AO. Novel strategy with potential to identify developmentally competent IVF blastocysts. Hum Reprod. 2008 ; 23 (8). 1748-1759
28. Cole LA, Seifer DB, Kardana A, Braunstein GD. Selecting human chorionic gonadotropin immunoassays: Consideration of cross-reacting molecules in first-trimester pregnancy serum and urine. Am J Obstet Gynecol. 1993 ; 168 (5). 1580-1586
29. Pandian R, Lu J, Ossolinska-Plewnia J. Fully automated chemiluminometric assay for hyperglycosylated human chorionic gonadotropin (invasive trophoblast antigen). Clin Chem. 2003 ; 49 (5). 808-810
30. Cole LA, Shahabi S, Oz UA, Bahado-Singh RO, Mahoney MJ. Hyperglycosylated human chorionic gonadotropin (invasive trophoblast antigen) immunoassay: A new basis for gestational down syndrome screening. Clin Chem. 1999 ; 45 (12). 2109-2119
31. Katz-Jaffe MG, Gardner DK. Symposium: Innovative techniques in human embryo viability assessment. can proteomics help to shape the future of human assisted conception?. Reprod Biomed Online. 2008 ; 17 (4). 497-501
32. Brison DR, Houghton FD, Falconer D, et al. Identification of viable embryos in IVF by non-invasive measurement of amino acid turnover. Hum Reprod. 2004 ; 19 (10). 2319-2324
33. Seli E, Sakkas D, Scott R, Kwok SC, Rosendahl SM, Burns DH. Noninvasive metabolomic profiling of embryo culture media using raman and near-infrared spectroscopy correlates with reproductive potential of embryos in women undergoing in vitro fertilization. Fertil Steril. 2007 ; 88 (5). 1350-1357
34. Soupart P, Noyes RW. Sialic acid as a component of the zona pellucida of the mammalian ovum. J Reprod Fertil. 1964 ; 8: 251-253
35. Sellens MH, Jenkinson EJ. Permeability of the mouse zona pellucida to immunoglobulin. J Reprod Fertil. 1975 ; 42 (1). 153-157
36. Turner K, Horobin RW. Permeability of the mouse zona pellucida: A structure-staining-correlation model using coloured probes. J Reprod Fertil. 1997 ; 111 (2). 259-265
37. Elliott MM, Kardana A, Lustbader JW, Cole LA. Carbohydrate and peptide structure of the alpha- and beta-subunits of human chorionic gonadotropin from normal and aberrant pregnancy and choriocarcinoma. Endocrine. 1997 ; 7 (1). 15-32
38. Bahado-Singh RO, Oz AU, Kingston JM, Shahabi S, Hsu CD, Cole L. The role of hyperglycosylated hCG in trophoblast invasion and the prediction of subsequent pre-eclampsia. Prenat Diagn. 2002 ; 22 (6). 478-481
39. Gras L, Trounson AO. Pregnancy and birth resulting from transfer of a blastocyst observed to have one pronucleus at the time of examination for fertilization. Hum Reprod. 1999 ; 14 (7). 1869-1871
40. Munne S, Cohen J. Chromosome abnormalities in human embryos. Hum Reprod Update. 1998 ; 4 (6). 842-855
41. Yu SL, Lee RK, Su JT, et al. Distinction between paternal and maternal contributions to the tripronucleus in human zygotes obtained after in vitro fertilization. Taiwan J Obstet Gynecol. 2006 ; 45 (4). 313-316
42. Lyall F. The human placental bed revisited. Placenta. 2002 ; 23 (8-9). 555-562
43. Rull K, Laan M. Expression of beta-subunit of HCG genes during normal and failed pregnancy. Hum Reprod. 2005 ; 20 (12). 3360-3368
44. Butler SA, Laidler P, Porter JR, et al. The beta-subunit of human chorionic gonadotrophin exists as a homodimer. J Mol Endocrinol. 1999 ; 22 (2). 185-192
45. Butler SA, Iles RK. The free monomeric beta subunit of human chorionic gonadotrophin (hCG beta) and the recently identified homodimeric beta-beta subunit (hCG beta beta) both have autocrine growth effects. Tumour Biol. 2004 ; 25 (1-2). 18-23