Development of a colorimetric and a fluorescence phosphatase-inhibitor assay suitable for drug discovery approaches

Journal of Biomolecular Screening

Volumn 18, Issue 8, 2013, Pages 899-909

  Sotoud, Hannieh ^a   Gribbon, Philip ^b   Ellinger, Bernhard ^b   Reinshagen, Jeanette ^b   Boknik, Peter ^c   Kattner, Lars ^d   El Armouche, Ali ^e   Eschenhagen, Thomas ^a  

^a UNIVERSITY MEDICAL CENTER HAMBURG EPPENDORF   (Germany)

^b European ScreeningPort GmbH   (Germany)

^c UNIVERSITY HOSPITAL MÜNSTER   (Germany)

^d Endotherm Life Science Molecules   (Germany)

^e UNIVERSITY MEDICAL CENTER GÖTTINGEN   (Germany)

Author keywords

DiFMUP; inhibitor 1; phosphatase inhibitor assay; pNPP; protein phosphatase type 1

Indexed keywords

CYCLIC AMP DEPENDENT PROTEIN KINASE; PHOSPHOPROTEIN PHOSPHATASE INHIBITOR; PHOSPHOPROTEIN PHOSPHATASE INHIBITOR 1; RECOMBINANT PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; COLORIMETRY; CONTROLLED STUDY; ENZYME ACTIVITY; FLUORESCENCE ANALYSIS; FLUORESCENCE PHOSPHATASE INHIBITOR ASSAY; HIGH THROUGHPUT SCREENING; PRIORITY JOURNAL; PROTEIN DEPHOSPHORYLATION; PROTEIN PHOSPHORYLATION;

DIFMUP; INHIBITOR-1; PHOSPHATASE-INHIBITOR ASSAY; PNPP; PROTEIN PHOSPHATASE TYPE-1;

COLORIMETRY; DRUG DISCOVERY; ENZYME ASSAYS; ENZYME INHIBITORS; INTRACELLULAR SIGNALING PEPTIDES AND PROTEINS; PROTEIN PHOSPHATASE 1;

EID: 84882317821     PISSN: 10870571     EISSN: 1552454X     Source Type: Journal    
DOI: 10.1177/1087057113486000     Document Type: Article

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