A mammalian expression system for high throughput antibody screening

Journal of Immunological Methods

Volumn 395, Issue 1-2, 2013, Pages 45-53

  Xu, Linda ^a   Jin, Xiaofang ^a   Rainey, G Jonah ^a   Wu, Herren ^a   Gao, Changshou ^a  

^a MEDIMMUNE   (United States)

Author keywords

Adenovirus expression system; High throughput screening; Mammalian expression; Phage display

Indexed keywords

ADENOVIRUS VECTOR; DNA FRAGMENT; HYBRID PROTEIN; IMMUNOGLOBULIN G; RESTRICTION ENDONUCLEASE; SINGLE CHAIN FRAGMENT VARIABLE ANTIBODY;

ANTIBODY SCREENING; ANTIGEN BINDING; ARTICLE; BACTERIOPHAGE; CELL STRAIN HEK293; CLONAL VARIATION; DNA VIRUS; GENE EXPRESSION SYSTEM; GENOTYPE PHENOTYPE CORRELATION; HIGH THROUGHPUT SCREENING; MAMMAL CELL; MOLECULAR CLONING; POLYMERASE CHAIN REACTION; PRIORITY JOURNAL; PROTEIN EXPRESSION; TRANSIENT EXPRESSION; VIRUS PARTICLE;

ADENOVIRUS EXPRESSION SYSTEM; CMV; CYTOMEGALOVIRUS.; EBNA-1; EPSTEIN-BARR VIRUS NUCLEAR ANTIGEN 1; FAB; FRAGMENT ANTIGEN-BINDING; HEAVY CHAIN VARIABLE REGIONS; HIGH THROUGHPUT; HIGH THROUGHPUT SCREENING; HTP; INTERNAL RIBOSOME ENTRY SITE; IRES; MAMMALIAN EXPRESSION; PHAGE DISPLAY; SCFV; SINGLE CHAIN FV; VH;

ADENOVIRUSES, HUMAN; ANTIBODIES; CELL LINE; EPSTEIN-BARR VIRUS NUCLEAR ANTIGENS; GENETIC VECTORS; HEK293 CELLS; HIGH-THROUGHPUT SCREENING ASSAYS; HUMANS; IMMUNOGLOBULIN FC FRAGMENTS; IMMUNOGLOBULIN G; PEPTIDE LIBRARY; RECOMBINANT PROTEINS; SINGLE-CHAIN ANTIBODIES;

ADENOVIRIDAE; MAMMALIA;

EID: 84881552515     PISSN: 00221759     EISSN: 18727905     Source Type: Journal    
DOI: 10.1016/j.jim.2013.06.009     Document Type: Article

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