Trapped intermediates in crystals of the FMN-dependent oxidase PhzG provide insight into the final steps of phenazine biosynthesis

Acta Crystallographica Section D: Biological Crystallography

Volumn 69, Issue 8, 2013, Pages 1403-1413

  Xu, Ningna ^a,b   Ahuja, Ekta Gayanji ^b,c   Janning, Petra ^b   Mavrodi, Dmitri Valeryevich ^d   Thomashow, Linda S ^e   Blankenfeldt, Wulf ^a,b  

^a UNIVERSITY OF BAYREUTH   (Germany)

^b MAX PLANCK INSTITUTE OF MOLECULAR PHYSIOLOGY   (Germany)

^c IKP Knowledgepark   (India)

^d WASHINGTON STATE UNIVERSITY   (United States)

^e AGRICULTURAL RESEARCH SERVICE   (United States)

Author keywords

antibiotics; flavins; hydride transfer; PdxH; reaction mechanism

Indexed keywords

1 PHENAZINECARBOXYLIC ACID; 1,6 PHENAZINEDICARBOXYLIC ACID; 1-PHENAZINECARBOXYLIC ACID; ANTIBIOTIC AGENT; BACTERIAL PROTEIN; FLAVINE MONONUCLEOTIDE; OXIDOREDUCTASE; PHENAZINE DERIVATIVE; PHENAZINE-1,6-DICARBOXYLATE; RIBOFLAVIN DERIVATIVE;

ARTICLE; BURKHOLDERIA; CHEMICAL STRUCTURE; CHEMISTRY; ENZYME SPECIFICITY; ENZYMOLOGY; HYDRIDE TRANSFER; METABOLISM; PDXH; PROTEIN CONFORMATION; PSEUDOMONAS FLUORESCENS; REACTION ANALYSIS; X RAY CRYSTALLOGRAPHY;

ANTIBIOTICS; FLAVINS; HYDRIDE TRANSFER; PDXH; REACTION MECHANISM;

BACTERIAL PROTEINS; BURKHOLDERIA; CRYSTALLOGRAPHY, X-RAY; FLAVIN MONONUCLEOTIDE; MODELS, MOLECULAR; OXIDOREDUCTASES; PHENAZINES; PROTEIN CONFORMATION; PSEUDOMONAS FLUORESCENS; SUBSTRATE SPECIFICITY;

EID: 84881301070     PISSN: 09074449     EISSN: 13990047     Source Type: Journal    
DOI: 10.1107/S0907444913008354     Document Type: Article

References (35)

1. Adams, P. D. et al. (2010). Acta Cryst. D66, 213-221
2. Ahuja, E. G., Janning, P., Mentel, M., Graebsch, A., Breinbauer, R., Hiller, W., Costisella, B., Thomashow, L. S., Mavrodi, D. V. & Blankenfeldt, W. (2008). J. Am. Chem. Soc. 130, 17053-17061
3. Blankenfeldt, W., Kuzin, A. P., Skarina, T., Korniyenko, Y., Tong, L., Bayer, P., Janning, P., Thomashow, L. S. & Mavrodi, D. V. (2004). Proc. Natl Acad. Sci. USA, 101, 16431-16436
4. Chen, V. B., Arendall, W. B., Headd, J. J., Keedy, D. A., Immormino, R. M., Kapral, G. J., Murray, L. W., Richardson, J. S. & Richardson, D. C. (2010). Acta Cryst. D66, 12-21
5. Salvo, M. L. di, Ko, T-P., Musayev, F. N., Raboni, S., Schirch, V. & Safo, M. K. (2002). J. Mol. Biol. 315, 385-397
6. Driscoll, J. A., Brody, S. L. & Kollef, M. H. (2007). Drugs, 67, 351-368
7. Emsley, P., Lohkamp, B., Scott, W. G. & Cowtan, K. (2010). Acta Cryst. D66, 486-501
8. Fordos, M. J. (1859). Rec. Trav. Soc. Emul. Sci. Pharm. 3, 30
9. Giddens, S. R., Feng, Y. & Mahanty, H. K. (2002). Mol. Microbiol. 45, 769-783
10. Ho Sui, S. J., Lo, R., Fernandes, A. R., Caulfield, M. D. G., Lerman, J. A., Xie, L., Bourne, P. E., Baillie, D. L. & Brinkman, F. S. L. (2012). Int. J. Antimicrob. Agents, 40, 246-251
11. Kabsch, W. (2010). Acta Cryst. D66, 125-132
12. Kazarinoff, M. N. & McCormick, D. B. (1975). J. Biol. Chem. 250, 3436-3442
13. Lau, G. W., Hassett, D. J., Ran, H. & Kong, F. (2004). Trends Mol. Med. 10, 599-606
14. Lau, G. W., Ran, H., Kong, F., Hassett, D. J. & Mavrodi, D. (2004). Infect. Immun. 72, 4275-4278
15. Laursen, J. B. & Nielsen, J. (2004). Chem. Rev. 104, 1663-1686
16. Li, Q-A., Mavrodi, D. V., Thomashow, L. S., Roessle, M. & Blankenfeldt, W. (2011). J. Biol. Chem. 286, 18213-18221
17. Mavrodi, D. V., Blankenfeldt, W. & Thomashow, L. S. (2006). Annu. Rev. Phytopathol. 44, 417-445
18. Mavrodi, D. V., Ksenzenko, V. N., Bonsall, R. F., Cook, R. J., Boronin, A. M. & Thomashow, L. S. (1998). J. Bacteriol. 180, 2541-2548
19. Mavrodi, D. V., Parejko, J. A., Mavrodi, O. V., Kwak, Y-S., Weller, D. M., Blankenfeldt, W. & Thomashow, L. S. (2013). Environ. Microbiol. 15, 675-686
20. McDonald, M., Mavrodi, D. V., Thomashow, L. S. & Floss, H. G. (2001). J. Am. Chem. Soc. 123, 9459-9460
21. Mentel, M., Ahuja, E. G., Mavrodi, D. V., Breinbauer, R., Thomashow, L. S. & Blankenfeldt, W. (2009). Chembiochem, 10, 2295-2304
22. Parsons, J. F., Calabrese, K., Eisenstein, E. & Ladner, J. E. (2003). Biochemistry, 42, 5684-5693
23. Parsons, J. F., Calabrese, K., Eisenstein, E. & Ladner, J. E. (2004). Acta Cryst. D60, 2110-2113
24. Parsons, J. F., Song, F., Parsons, L., Calabrese, K., Eisenstein, E. & Ladner, J. E. (2004). Biochemistry, 43, 12427-12435
25. Pierson, L. S., Gaffney, T., Lam, S. & Gong, F. (1995). FEMS Microbiol. Lett. 134, 299-307
26. Price-Whelan, A., Dietrich, L. E. & Newman, D. K. (2006). Nature Chem. Biol. 2, 71-78
27. Price-Whelan, A., Dietrich, L. E. & Newman, D. K. (2007). J. Bacteriol. 189, 6372-6381
28. Rui, Z., Ye, M., Wang, S., Fujikawa, K., Akerele, B., Aung, M., Floss, H. G., Zhang, W. & Yu, T. W. (2012). Chem. Biol. 19, 1116-1125
29. Safo, M. K., Musayev, F. N., di Salvo, M. L. & Schirch, V. (2001). J. Mol. Biol. 310, 817-826
30. Saleh, O., Gust, B., Boll, B., Fiedler, H. P. & Heide, L. (2009). J. Biol. Chem. 284, 14439-14447
31. Sinnokrot, M. O., Valeev, E. F. & Sherrill, C. D. (2002). J. Am. Chem. Soc. 124, 10887-10893
32. Turner, J. M. & Messenger, A. J. (1986). Adv. Microb. Ecol. 27, 211-275
33. Vagin, A. & Teplyakov, A. (2010). Acta Cryst. D66, 22-25
34. Weiss, M. S. (2001). J. Appl. Cryst. 34, 130-135
35. Winn, M. D. et al. (2011). Acta Cryst. D67, 235-242