Peptide microarrays for profiling of serine/threonine kinase activity of recombinant kinases and lysates of cells and tissue samples

Methods in Molecular Biology

Volumn 977, Issue , 2013, Pages 259-271

  Hilhorst, Riet ^a   Houkes, Liesbeth ^a   Mommersteeg, Monique ^a   Musch, Joyce ^a   Van Den Berg, Adriënne ^a   Ruijtenbeek, Rob ^a  

^a NONE

Author keywords

Kinase activity; Kinase activity profiling; Kinase inhibition; Kinase kinetics; Kinase substrate identification; Multiplex assay; Peptide microarray; Serine threonine kinase; Tyrosine kinase

Indexed keywords

PHOSPHOTRANSFERASE; PROTEIN SERINE THREONINE KINASE; CELL EXTRACT; IMMOBILIZED PROTEIN; PEPTIDE FRAGMENT; RECOMBINANT PROTEIN;

ARTICLE; CELL LYSATE; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME SUBSTRATE; MICROARRAY ANALYSIS; PEPTIDE ANALYSIS; PRIORITY JOURNAL; ANIMAL; CELL FRACTIONATION; CELL STRAIN MCF 7; CHEMISTRY; ENZYME ASSAY; HUMAN; KINETICS; METHODOLOGY; PHOSPHORYLATION; PROTEIN MICROARRAY; PROTEIN PROCESSING;

ANIMALS; CELL EXTRACTS; CELL FRACTIONATION; ENZYME ASSAYS; HUMANS; IMMOBILIZED PROTEINS; KINETICS; MCF-7 CELLS; PEPTIDE FRAGMENTS; PHOSPHORYLATION; PROTEIN ARRAY ANALYSIS; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEIN-SERINE-THREONINE KINASES; RECOMBINANT PROTEINS;

MLCS; MLOWN;

EID: 84881000119     PISSN: 10643745     EISSN: None     Source Type: Book Series    
DOI: 10.1007/978-1-62703-284-1_21     Document Type: Article

References (19)

1. Thiele A, Stangl GI, Schutkowski M. (2011) Deciphering enzyme function using peptide arrays. Mol Biotechnol 49: 283-305
2. Arsenault R, Griebel P, Napper S. (2011) Peptide arrays for kinome analysis: new opportunities and remaining challenges. Proteomics 11: 4595-4609
3. Lemeer S, Jopling C, Naji F et al (2007) Protein-tyrosine kinase activity profiling in knock down zebrafish embryos. PLoS One 2: e581
4. Versele M, Talloen W, Rockx C et al (2009) Response prediction to a multitargeted kinase inhibitor in cancer cell lines and xenograft tumors using high-content tyrosine peptide arrays with a kinetic readout. Mol Cancer Ther 8: 1846-1855
5. Hilhorst R, Houkes L, van den Berg A et al (2009) Peptide microarrays for detailed, high-throughput substrate identification, kinetic characterization, and inhibition studies on protein kinase A. Anal Biochem 387: 150-161
6. Sanz A, Ungureanu D, Pekkala T et al (2011) Analysis of Jak2 catalytic function by peptide microarrays: the role of the JH2 domain and V617F mutation. PLoS One 6: e18522
7. Poot AJ, van Ameijde J, Slijper M et al (2009) Development of selective bisubstrate-based inhibitors against protein kinase C (PKC) isozymes by using dynamic peptide microarrays. Chembiochem 10: 2042-2051
8. Harmsen S, Dolman ME, Nemes Z et al (2011) Development of a cell-selective and intrinsically active multikinase inhibitor bioconjugate. Bioconjug Chem 22: 540-545
9. Sikkema AH, Diks SH, den Dunnen WF et al (2009) Kinome profiling in pediatric brain tumors as a new approach for target discovery. Cancer Res 69: 5987-5995
10. Bratland A, Boender PJ, Hoifodt HK et al (2009) Osteoblast-induced EGFR/ERBB2 signaling in androgen-sensitive prostate carcinoma cells characterized by multiplex kinase activity profiling. Clin Exp Metastasis 26: 485-496
11. Plaza-Menacho I., Morandi A, Mologni L et al (2011) Focal adhesion kinase (FAK) binds RET kinase via its FERM domain, priming a direct and reciprocal RET-FAK transactivation mechanism. J Biol Chem 286: 17292-17302
12. Maat W, el Filali M, Dirks-Mulder A et al (2009) Episodic Src activation in uveal melanoma revealed by kinase activity profiling. Br J Cancer 101: 312-319
13. Saelen MG, Flatmark K, Folkvord S et al (2011) Tumor kinase activity in locally advanced rectal cancer: angiogenic signaling and early systemic dissemination. Angiogenesis 14: 481-489
14. Ter Elst A., Diks SH, Kampen KR et al (2011) Identification of new possible targets for leukemia treatment by kinase activity profiling. Leuk Lymphoma 52: 122-130
15. Jinnin M, Medici D, Park L et al (2008) Suppressed NFAT-dependent VEGFR1 expression and constitutive VEGFR2 signaling in infantile hemangioma. Nat Med 14: 1236-1246
16. Folkvord S, Flatmark K, Dueland S et al (2010) Prediction of response to preoperative chemoradiotherapy in rectal cancer by multiplex kinase activity profiling. Int J Radiat Oncol Biol Phys 78: 555-562
17. Hilhorst R, Schaake E, van Pel R et al (2011) Application of kinase activity profiles to predict response to erlotinib in a neoadjuvant setting in early stage non-small cell lung cancer (NSCLC). J Clin Oncol 28: suppl. abstract 10566
18. Hilhorst R, Schaake E., van Pel R et al (2011) Blind prediction of response to erlotinib in early stage non-small cell lung cancer (NSCLC) in a neoadjuvant settin based on kinase activity profiles. J Clin Oncol 29: suppl abstract 10521
19. Lemeer S, Ruijtenbeek R, Pinkse MW et al (2007) Endogenous phosphotyrosine signaling in zebrafish embryos. Mol Cell Proteomics 6: 2088-2099