Rapid real-time PCR detection of transgenic cry1C rice using plasmid molecule as calibrator

European Food Research and Technology

Volumn 237, Issue 2, 2013, Pages 101-107

  Wang, Hui ^a   Qian, Chong ^a   Su, Changqing ^b   Duan, Yuxi ^a   Bai, Hongzhi ^a  

^a SHENYANG AGRICULTURAL UNIVERSITY   (China)

^b HENGSHUI UNIVERSITY   (China)

Author keywords

cry1C rice; Event specific PCR; Plasmid molecule; Real time PCR

Indexed keywords

BACTERIOLOGY; DNA; GENES; STATISTICS;

BACILLUS THURINGIENSIS; CRY1C RICE; DETECTION METHODS; EVENT-SPECIFIC PCR; LIMIT OF QUANTIFICATIONS; REAL TIME POLYMERASE CHAIN REACTIONS; REAL-TIME PCR; STANDARD DEVIATION;

POLYMERASE CHAIN REACTION;

BACILLUS THURINGIENSIS; CNAPHAROCROSIS MEDINALIS;

EID: 84880812010     PISSN: 14382377     EISSN: 14382385     Source Type: Journal    
DOI: 10.1007/s00217-013-1957-2     Document Type: Article

References (32)

1. James C (2011) Global status of commercialized Biotech/GM crops. ISAAA Briefs 43.
2. Matsuoka T (2001) GMO labeling and detection methods in Japan. APEC-JIRCAS Joint symposium and workshop on Agricultural Biotechnology.
3. Notification 2000-31. (2000) Ministry of Agriculture and Forestry of Korea, Seoul, Korea.
4. Commission E (2003) Concerning on genetically modified food and feed. Commission Regulation (EC)No. 1829/2003 of September 22, 2003. Off J Eur Commun L268: 1-23.
5. Commission E (2003b) Concerning the traceability and labelling of genetically modified organisms and the traceability of food and feed products produced from genetically modified organisms and amending Directive. Commission Regulation (EC) No. 1830/2003 of September 22, 2003. 2001/18/EC. Off J Eur Commun L268.
6. Marmiroli N, Maestri E, Gulli M, Malcevschi A, Peano C, Bordoni R, Bellis GD (2008) Methods for detection of GMOs in food and feed. Anal Bioanal Chem 392: 369-384.
7. Holst-Jensen A, Rønning SB, Løvseth A, Berdal KG (2003) PCR technology for screening and quantification of genetically modified organisms (GMOs). Anal Bioanal Chem 375(8): 985-993.
8. Yang LT, Shen HF, Pan AH, Chen JX, Huang C, Zhang DB (2005) Screening and construct-specific detection methods of transgenic Huafan No 1 tomato by conventional and real-time PCR. J Sci Food Agr 85: 2159-2166.
9. Wu G, Wu Y, Xiao L, Lu C (2008) Event-specific qualitative and quantitative PCR methods for the detection of genetically modified rapeseed Oxy-235. Transgenic Res 17: 851-862.
10. Yang LT, Pan AH, Zhang KW, Guo JC, Yin CS, Chen JX, Huang C, Zhang DB (2005) Identification and quantification of three genetically modified insect resistant cotton lines using conventional and TaqMan real-time polymerase chain reaction methods. J Agric Food Chem 53: 6222-6229.
11. Su CQ, Sun Y, Xie JJ, Peng YF (2011) A construct-specific qualitative and quantitative PCR detection method of transgenic maize BVLA430101. Eur Food Res Technol 233: 117-122.
12. Berdal KG, Holst-Jensen A (2001) Roundup Ready® soybean event-specific real-time quantitative PCR assay and estimation of the practical detection and quantification limits in GMO analyses. Eur Food Res Technol 213: 432-438.
13. Kuribara H, Shindo Y, Matsuoka T, Takubo K, Futo S, Aoki N, Hirao T, Akiyama H, Goda Y, Toyada M, Hino A (2002) Novel reference molecules for quantification of genetically modified maize and soybean. J AOAC Int 85(5): 1077-1089.
14. Corbisier P, Broeders S, Charels D, Trapmann S, Vincent S, Emons H (2007) Certification of plasmidic DNA containing MON 810 maize DNA fragments, ERMR-AD413. European Commission. Joint Research Centre, Institute for Reference Materials and Measurements.
15. Yang L, Pan A, Zhang K, Yin CS, Qian BJ, Chen JX, Huang C, Zhang DB (2005) Qualitative and quantitative PCR methods for event-specific detection of genetically modified cotton Mon1445 and Mon531. Transgenic Res 14: 817-831.
16. http://www. stee. agri. gov. cn/biosafety/spxx/P020091127591594596689. pdf, last Accessed 8 Sep 2012.
17. Babekova R, Funk T, Pecoraro S, Engel K-H, Busch U (2009) Development of an event-specific Real-time PCR detection method for the transgenic Bt rice line KMD1. Eur Food Res Technol 228: 707-716.
18. Su C, Xie J, Wang X, Peng Y (2011) Integrated structure and event-specific real-time detection of transgenic cry1Ac/SCK rice Kefeng 6. Eur Food Res Technol 232: 351-359.
19. Cao Y, Wu G, Wu Y, Nie S, Zhang L, Lu C (2011) Characterization of the transgenic rice event TT51-1 and construction of a reference plasmid. J Agric Food Chem 59(16): 8550-8559.
20. Wang W, Zhu T, Lai F, Fu Q (2012) Event-specific qualitative and quantitative detection of transgenic rice Kefeng-8 by characterization of the transgene flanking sequence. Eur Food Res Technol 234: 477-484.
21. Tang W, Chen H, Xu C, Li X, Lin Y, Zhang Q (2006) Development of insect-resistant transgenic indica rice with a synthetic cry1C* gene. Mol Breeding 18: 1-10.
22. Hernández M, Esteve T, Pla M (2005) Real-time polymerase chain reaction based assays for quantitative detection of barley, rice, sunflower, and wheat. J Agric Food Chem 53(18): 7003-7009.
23. Lee C, Kim J, Shin SG, Hwang S (2006) Absolute and relative QPCR quantification of plasmid copy number in Escherichia coli. J Biotechnol 123: 273-280.
24. ENGL (European Network of GMO Laboratories) (2008) Definition of minimum performance requirements for analytical methods of GMO testing. http://gmocrl. jrc. ec. europa. eu/guidancedo/Min_Perf_Requir_Analyt_methods. pdf.
25. International Organization for Standardization(ISO)5725, 1994. Accuracy (trueness and precision) of measurement methods and results. International Organization for standardisation, Genéve, Switzerland.
26. Huang C-C, Pan T-M (2005) Event-specific real-time detection and quantification of genetically modified roundup ready soybean. J Agric Food Chem 53(10): 3833-3839.
27. Taverniers I, Bockstaele EV, Loose MD (2004) Cloned plasmid DNA fragments as calibrators for controlling GMOs: different real-time duplex quantitative PCR methods. Anal Bioanal Chem 378: 1198-1207.
28. Nielsen CR, Berdal KG, Holst-Jensen A (2004) Characterisation of the 5′ integration site and development of an event-specific real-time PCR assay for NK603 maize from a low starting copy number. Eur Food Res Technol 219(4): 421-427.
29. Taverniers I, Windels P, Vaitilingom M, Milcamps A, van Bockstaele E, van den Eede G, De Loose M (2005) Event-specific plasmid standards and real-time PCR methods for transgenic Bt11, Bt176, and GA21 maize and transgenic GT73 canola. J Agric Food Chem 53(8): 3041-3052.
30. Mattarucchi E, Weighardt F, Barbati C, Querci M, van den Eede G (2005) Development and applications of real-time PCR standards for GMO quantification based on tandem-marker plasmids. Eur Food Res Technol 221(3-4): 511-519.
31. Rossen L, Nørskov P, Holmstrøm K, Rasmussen OF (1992) Inhibition of PCR by components of food samples, microbial diagnostic assays and DNA-extraction solutions. Int J Food Micobiol 17(1): 37-45.
32. Cankar K, Štebih D, Dreo T, Žel J, Gruden K (2006) Critical points of DNA quantification by real-time PCR-effects of DNA extraction method and sample matrix on quantification of genetically modified organisms. BMC Biotechnol 6: 37.