Event-specific real-time detection and quantification of genetically modified roundup ready soybean

Journal of Agricultural and Food Chemistry

Volumn 53, Issue 10, 2005, Pages 3833-3839

  Huang, Chia Chia ^a   Pan, Tzu Ming ^a  

^a NATIONAL TAIWAN UNIVERSITY   (Taiwan)

Author keywords

Genetically modified organisms (GMOs); Light upon extension (LUX); Real time PCR; RRS

Indexed keywords

GLYCINE MAX;

PLANT DNA;

ARTICLE; GENETICS; METHODOLOGY; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOYBEAN; TRANSGENIC PLANT;

DNA, PLANT; PLANTS, GENETICALLY MODIFIED; POLYMERASE CHAIN REACTION; SENSITIVITY AND SPECIFICITY; SOYBEANS;

EID: 20844462619     PISSN: 00218561     EISSN: None     Source Type: Journal    
DOI: 10.1021/jf048580x     Document Type: Article

References (29)

1. James, C. Global status of commercialized transgenic crops: 2003. ISAAA Briefs 2003, 30.
2. Pan, T. M. The impact and advantage of genetically modified crop. Bioindustry 2002, 13, 441-451.
3. Anklam, E.; Gadani, F.; Heinze, P.; Pijnenburg, H.; Eede, G. V. D. Analytical methods for detection and determination of genetically modified organisms in agricultural crops and plant-derived food products. Eur. Food Res. Technol. 2002, 214, 3-26.
4. Jung, R.; Soondrum, K.; Neumaier, M. Quantitative PCR. Clin. Chem. Lab. Med. 2000, 38, 833-836.
5. Nazarenko, I.; Pires, R.; Lowe, B.; Obaidy, M.; Rashtchian, A. Effect of primary and secondary structure of oligodeoxyribonucleotides on the fluorescent properties of conjugated dyes. Nucleic Acids Res. 2002, 30, 2089-2195.
6. Mackay, I. M.; Arden, K. E.; Nitsche, A. Real-time PCR in virology. Nucleic Acids Res. 2002, 30, 1295-1305.
7. Nazarenko, I.; Lowe, B.; Darfler, M.; Ikonomi, P.; Schuster, D.; Rashtchian, A. Multiplex quantitative PCR using self-quenched primers labeled with a single fluorophore. Nucleic Acids Res. 2002, 30, e37.
8. Lowe, B.; Avila, H. A.; Bloom, F. B.; Gleeson, M.; Kusser, W. Quantitaten of gene expression in neural precursors by reverse-transcription polymerase chain reaction using self-quenched, fluorogenic primers. Anal. Biochem. 2003, 315, 95-105.
9. Windels, P.; Taverniers, I.; Depicker, A.; Van Bockstaele, E.; De Loose, M. Characterisation of the Roundup Ready soybean insert. Eur. Food Res. Technol. 2001, 213, 107-112.
10. Lipp, M.; Brodmann, P.; Pietsch, K.; Pauwels, J.; Anklam, E. IUPAC collaborative trial study of a method to detect genetically modified soybeans and maize in dried powder. J. AOAC Int. 1999, 82, 923-928.
11. Kuribara, H.; Shindo, Y.; Matsuoka, T.; Takubo, K.; Futo, S.; Aoki, N.; Hirao, T.; Akiyama, H.; Goda, Y.; Toyoda, M.; Hino, A. Novel reference molecules for quantitation of genetically modified maize and soybean. J. AOAC Int. 2002, 85, 1077-1189.
12. Plant DNA C-values Database, 2004; http://www.rbgkew.org.uk/cval/database1.html.
13. Rønning, S. B.; Vatilingom, M.; Berdal, K. G.; Holst-Jensen, A. Event specific real-time quantitative PCR for genetically modified Bt11 maize (Zea mays). Eur. Food Res. Technol. 2003, 216, 347-354.
14. Hernández, M.; Esteve, T.; Prat, S.; Pla, M. Development of real-time PCR systems based on SYBR Green I, Amplifluor and TaqMan technologies for specific quantitative detection of the transgenic maize event GA21. J. Cereal Sci. 2004, 39, 99-107.
15. Vaïtilingom, M.; Pijnenburg, H.; Gendre, F.; Brignon, P. Real-time quantitative PCR detection of genetically modified Maximizer maize and Roundup Ready soybean in some representative foods. J. Agric. Food Chem. 1999, 47, 5261-5266.
16. Institute for Health Protection of Consumers and Veterinary Medicine (BgVV). Development of qualitative as well as quantitative detection methods to identify a genetic modification in soybean and maize products. Report of the EU tender N. XXIV/9898A3/001, 2000.
17. Terry, C. F.; Harris, N. Event-specific detection of Roundup Ready Soya using two different real time PCR detection chemistries. Eur. Food Res. Technol. 2001, 213, 425-431.
18. Berdal, K. G.; Holst-Jensen, A. Roundup Ready soybean event-specific real-time quantitative PCR assay and estimation of the practical detection and quantification limits in GMO analyses. Eur. Food Res. Technol. 2001, 213, 432-438.
19. Huang, H. Y.; Pan, T. M. Detection of genetically modified maize MON810 and NK603 by multiplex and real-Time polymerase chain reaction methods. J. Agric. Food Chem. 2004, 52, 3264-3268.
20. Terry, C. F.; Shanahan, D. J.; Ballam, L. D.; Harris, N.; McDowell, D. G.; Parkes, H. C. Real-time detection of genetically modified soya using LightCycler and ABI 7700 platforms with TaqMan, Scorpion and SYBR Green I chemistries. J. AOAC. Int. 2002, 85, 938-944.
21. Permingeat, H. R.; Reggiardo, M. I.; Vallejos, R. H. Detection and quantification of transgenes in grains by multiplex and real-time PCR. J. Agric. Food Chem. 2002, 50, 4431-4436.
22. Hernández, M.; Rodríguez-Lázaro, D.; Esteve, T.; Prat, S.; Pla, M. Development of melting temperature-based SYBR Green I Polymerase Chain Reaction methods for multiplex genetically modified organism detection. Anal. Biochem. 2003, 323, 164-170.
23. Taverniers, I.; Windels, P.; Bockstaele, E. V.; Loose, M. D. Use of cloned DNA fragments for event-specific quantification of genetically modified organisms in pure and mixed food products. Eur. Food Res. Technol. 2001, 213, 417-424.
24. Pardigol, A.; Guillet, S.; Popping, B. A simple procedure for quantification of genetically modified organisms using hybrid amplicon standards. Eur. Food Res. Technol. 2003, 216, 412-420.
25. Rho, J. K.; Lee, T.; Jung, S. I.; Kim, T. S.; Park, Y. H.; Kim, Y. M. Qualitative and quantitative PCR methods for detection of three lines of genetically modified potaoes. J. Agric. Food Chem. 2004, 52, 3269-3274.
26. Holst-Jensen, A.; Rønning, S. B.; Løvseth, A.; Berdal, K. G. PCR technology for screening and quantification of genetically modified organisms (GMOs). Anal. Bioanal. Chem. 2003, 375, 985-993.
27. Eede, G. V. D.; Kay, S.; Anklam, E. Analytical challenges: bridging gap from regulation to enforcement. J. AOAC Int. 2002, 85, 757-761.
28. Shindo, Y.; Kuribara, H.; Matsuoka, T.; Futo, S.; Sawada, C.; Shono, J.; Akiyama, H.; Goda, Y.; Toyoda, M.; Hino, A. Validation of real-time PCR analyses for line-specific quantitation of genetically modified maize and soybean using new reference molecules. J. AOAC Int. 2002, 85, 1119-1126.
29. Monsanto. 2004; http://www.monsanto.com/monsanto/content/our_pledge/transparency/prod_safety/ roundup_soybean/pss.pdf.