ArhGAP15, a rac-specific gtpase-activating protein, plays a dual role in inhibiting small GTpase signaling

Journal of Biological Chemistry

Volumn 288, Issue 29, 2013, Pages 21117-21125

  Radu, Maria ^a   Rawat, Sonali J ^b   Beeser, Alexander ^a,d   Iliuk, Anton ^c   Tao, Weiguo Andy ^c   Chernoff, Jonathan ^a  

^a FOX CHASE CANCER CENTER   (United States)

^b DREXEL UNIVERSITY COLLEGE OF MEDICINE   (United States)

^c PURDUE UNIVERSITY   (United States)

^d KANSAS STATE UNIVERSITY   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

DIRECT INTERACTIONS; DUAL ROLE; GTP HYDROLYSIS; GTPASE-ACTIVATING PROTEIN; P-21 ACTIVATED KINASE; SMALL GTPASE;

BIOCHEMISTRY; BIOLOGY;

HYDROLYSIS;

ARHGAP15 PROTEIN; GUANOSINE TRIPHOSPHATASE ACTIVATING PROTEIN; MITOGEN ACTIVATED PROTEIN KINASE; P21 ACTIVATED KINASE 1; P21 ACTIVATED KINASE 2; RAC1 PROTEIN; UNCLASSIFIED DRUG;

ARTICLE; COMPLEX FORMATION; CONTROLLED STUDY; ENZYME INHIBITION; ENZYME SUBSTRATE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN DOMAIN; PROTEIN MICROARRAY; PROTEIN PHOSPHORYLATION; PROTEIN PROTEIN INTERACTION; SIGNAL TRANSDUCTION;

ERK; PROTEIN KINASES; PROTEIN PHOSPHORYLATION; SIGNAL TRANSDUCTION; SMALL GTPASES;

AMINO ACID SEQUENCE; DOWN-REGULATION; EXTRACELLULAR SIGNAL-REGULATED MAP KINASES; GTPASE-ACTIVATING PROTEINS; HEK293 CELLS; HUMANS; MAP KINASE SIGNALING SYSTEM; MODELS, BIOLOGICAL; MOLECULAR SEQUENCE DATA; P21-ACTIVATED KINASES; PHOSPHORYLATION; PROTEIN BINDING; PROTEIN STRUCTURE, TERTIARY; RAC GTP-BINDING PROTEINS; SIGNAL TRANSDUCTION; SUBSTRATE SPECIFICITY;

EID: 84880535369     PISSN: 00219258     EISSN: 1083351X     Source Type: Journal    
DOI: 10.1074/jbc.M113.459719     Document Type: Article

References (39)

1. Guilluy, C., García-Mata, R., and Burridge, K. (2011) Rho protein crosstalk: another social network? Trends Cell Biol. 21, 718-726
2. Pai, S. Y., Kim, C., and Williams, D. A. (2010) Rac GTPases in human diseases. Dis. Markers 29, 177-187
3. Rathinam, R., Berrier, A., and Alahari, S. K. (2011) Role of Rho GTPases and their regulators in cancer progression. Front. Biosci. 16, 2561-2571
4. Bobak, D., Moorman, J., Guanzon, A., Gilmer, L., and Hahn, C. (1997) Inactivation of the small GTPase Rho disrupts cellular attachment and induces adhesion-dependent and adhesion-independent apoptosis. Oncogene 15, 2179-2189
5. Gamblin, S. J., and Smerdon, S. J. (1998) GTPase-activating proteins and their complexes. Curr. Opin. Struct. Biol. 8, 195-201
6. Lamarche, N., and Hall, A. (1994) GAPs for rho-related GTPases. Trends Genet. 10, 436-440
7. García-Mata, R., and Burridge, K. (2007) Catching a GEF by its tail. Trends Cell Biol. 17, 36-43
8. Siderovski, D. P., and Willard, F. S. (2005) The GAPs, GEFs, and GDIs of heterotrimeric G-protein a-subunits. Int. J. Biol. Sci. 1, 51-66
9. Garcia-Mata, R., Boulter, E., and Burridge, K. (2011) The 'invisible hand': regulation of RHO GTPases by RHOGDIs. Nat. Rev. Mol. Cell Biol. 12, 493-504
10. Moon, S. Y., and Zheng, Y. (2003) Rho GTPase-activating proteins in cell regulation. Trends Cell Biol. 13,13-22
11. Nethe, M., and Hordijk, P. L. (2010) The role of ubiquitylation and degradation in RhoGTPase signalling. J. Cell Sci. 123, 4011-4018
12. Ladwein, M., and Rottner, K. (2008) On the Rho'd: the regulation of membrane protrusions by Rho-GTPases. FEBS Lett. 582, 2066-2074
13. Ridley, A. J. (2006) Rho GTPases and actin dynamics in membrane protrusions and vesicle trafficking. Trends Cell Biol. 16, 522-529
14. Arias-Romero, L. E., and Chernoff, J. (2008) A tale of two Paks. Biol. Cell 100, 97-108
15. Guo, D., Zhang, J. J., and Huang, X. Y. (2010) A newRac/PAK/GC/cGMP signaling pathway. Mol. Cell Biochem. 334, 99-103
16. Hofmann, C., Shepelev, M., and Chernoff, J. (2004) The genetics of Pak. J. Cell Sci. 117, 4343-4354
17. Manser, E., Loo, T. H., Koh, C. G., Zhao, Z. S., Chen, X. Q., Tan, L., Tan, I., Leung, T., and Lim, L. (1998) PAK kinases are directly coupled to the PIX family of nucleotide exchange factors. Mol. Cell 1,183-192
18. Zenke, F. T., Krendel, M., DerMardirossian, C., King, C. C., Bohl, B. P., and Bokoch, G. M. (2004) p21-activated kinase 1 phosphorylates and regulates 14-3-3 binding to GEF-H1, a microtubule-localized Rho exchange factor. J. Biol. Chem. 279,18392-18400
19. DerMardirossian, C. M., and Bokoch, G. M. (2006) Phosphorylation of RhoGDI by p21-activated kinase 1. Methods Enzymol. 406, 80 -90
20. Szczepanowska, J. (2009) Involvement of Rac/Cdc42/PAK pathway in cy-toskeletal rearrangements. Acta Biochim. Pol. 56, 225-234
21. Arias-Romero, L. E., Villamar-Cruz, O., Pacheco, A., andKosoff, R. (2010) A Rac-Pak signaling pathway is essential for ErbB2-mediated transformation of human breast epithelial cancer cells. Oncogene 43, 5839 -5849
22. Van den Broeke, C., Radu, M., Nauwynck, H. J., Chernoff, J., and Favoreel, H. W. (2011) Virus Res. 155, 376-380
23. Iliuk, A. B., Martin, V. A., Alicie, B. M., Geahlen, R. L., and Tao, W. A. (2010) In-depth analyses of kinase-dependent tyrosine phosphopro-teomes based on metal ion-functionalized soluble nanopolymers. Mol. Cell Proteomics 9, 2162-2172
24. De la Mota-Peynado, A., Chernoff, J., and Beeser, A. (2011) Identification of the atypical MAPK Erk3 as a novel substrate for p21-activated kinase (Pak) activity. J. Biol. Chem. 286, 13603-13611
25. Seoh, M. L., Ng, C. H., Yong, J., Lim, L., and Leung, T. (2003) ArhGAP15, a novel human RacGAP protein with GTPase binding property. FEBS Lett.539,131-137
26. Iliuk, A., Jayasundera, K., Schluttenhofer, R., and Tao, W. A. (2011) Func-tionalized soluble nanopolymers for phosphoproteome analysis. Methods Mol. Biol. 790 277-285
27. Johnsson, N., Marriott, G., and Weber, K. (1988) p36, the major cytoplasmic substrate of src tyrosine protein kinase, binds to its p11 regulatory subunit via a short amino-terminal amphiphatic helix. EMBO J. 7 2435-2442
28. Geltz, N. R., and Augustine, J. A. (1998) The p85 and p110 subunits of phosphatidylinositol 3-kinase-a are substrates, in vitro, for a constitu-tively associated protein tyrosine kinase in platelets. Blood 91 930 -939
29. Manser, E., Leung, T., Salihuddin, H., Zhao, Z. S., and Lim, L. (1994) A brain serine/threonine protein kinase activated by Cdc42 and Rac1. Nature 367, 40 - 46
30. Sells, M. A., Knaus, U. G., Bagrodia, S., Ambrose, D. M., Bokoch, G. M., and Chernoff, J. (1997) Human p21-activated kinase (Pak1) regulates actin organization in mammalian cells. Curr. Biol. 7, 202-210
31. Murray, B. W., Guo, C., Piraino, J., Westwick, J. K., Zhang, C., Lamerdin, J., Dagostino, E., Knighton, D., Loi, C. M., Zager, M., Kraynov, E., Popoff, I., Christensen, J. G., Martinez, R., Kephart, S. E., Marakovits, J., Karlicek, S., Bergqvist, S., and Smeal, T. (2010) Small-molecule p21-activated kinase inhibitor PF-3758309 is a potent inhibitor of oncogenic signaling and tumor growth. Proc. Natl. Acad. Sci. U. S. A. 107, 9446 -9451
32. Chow, H. Y., Jubb, A. M., Koch, J. N., Jaffer, Z. M., Stepanova, D., Campbell, D. A., Duron, S. G., O'Farrell, M., Cai, K. Q., Klein-Szanto, A. J., Gutkind, J. S., Hoeflich, K. P., and Chernoff, J. (2012) p21-Activated kinase 1 is required for efficient tumor formation and progression in a Ras-me-diated skin cancer model. Cancer Res. 72 5966-5975
33. Bernards, A., and Settleman, J. (2004) GAP control: regulating the regulators of small GTPases. Trends Cell Biol. 14 377-385
34. Ding, F., Yin, Z., and Wang, H. R. (2011) Ubiquitination in Rho signaling. Curr. Top Med. Chem. 11 2879-2887
35. Soncini, C., Berdo, I., and Draetta, G. (2001) Ras-GAP SH3 domain binding protein (G3BP) is a modulator of USP10, a novel human ubiquitin specific protease. Oncogene 20, 3869-3879
36. Costa, C., Germena, G., Martin-Conte, E. L., Molineris, I., Bosco, E., Marengo, S., Azzolino, O., Altruda, F., Ranieri, V. M., and Hirsch, E. (2011) TheRacGAP ArhGAP15 is a master negative regulator of neutrophil functions. Blood 118,1099-1108
37. Rennefahrt, U. E., Deacon, S. W., Parker, S. A., Devarajan, K., Beeser, A., Chernoff, J., Knapp, S., Turk, B. E., and Peterson, J. R. (2007) Specificity profiling of Pak kinases allows identification of novel phosphorylation sites. J. Biol. Chem. 282, 15667-15678
38. Xiao, G. H., Beeser, A., Chernoff, J., and Testa, J. R. (2002) p21-activated kinase links Rac/Cdc42 signaling to merlin. J. Biol. Chem. 277 883- 886
39. Rong, R., Surace, E. I., Haipek, C. A., Gutmann, D. H., and Ye, K. (2004) Serine 518 phosphorylation modulates merlin intramolecular association and binding to critical effectors important for NF2 growth suppression. Oncogene 23, 8447-8454