Improvement of NADPH bioavailability in Escherichia coli through the use of phosphofructokinase deficient strains

Applied Microbiology and Biotechnology

Volumn 97, Issue 15, 2013, Pages 6883-6893

  Wang, Yipeng ^a   San, Ka Yiu ^b   Bennett, George N ^a  

^a RICE UNIVERSITY   (United States)

^b Rice University   (United States)

Author keywords

E. coli; G6PDH; NADPH bioavailability; PFK; pfkA; pfkB

Indexed keywords

E. COLI; G6PDH; NADPH BIOAVAILABILITY; PFK; PFKA; PFKB;

BIOCHEMISTRY; BIOSYNTHESIS; ESCHERICHIA COLI; FRUCTOSE;

STRAIN;

6 PHOSPHOFRUCTOKINASE; 6 PHOSPHOFRUCTOKINASE ISOENZYME L; 6 PHOSPHOFRUCTOKINASE ISOENZYME M; ACRYLIC ACID DERIVATIVE; FRUCTOSE 6 PHOSPHATE; GLUCOSE 6 PHOSPHATE DEHYDROGENASE; LACTATE DEHYDROGENASE; LYCOPENE; OXIDOREDUCTASE; PENTOSE PHOSPHATE; PROPIONIC ACID DERIVATIVE; REDUCED NICOTINAMIDE ADENINE DINUCLEOTIDE PHOSPHATE;

BIOAVAILABILITY; BIOCHEMISTRY; BIOLOGICAL PRODUCTION; CARBOHYDRATE; COLIFORM BACTERIUM; GENE EXPRESSION; INDUSTRIAL PRODUCTION; PHOSPHATE; PIGMENT; REDUCTION;

ARTICLE; BACTERIAL STRAIN; BIOAVAILABILITY; BIOSYNTHESIS; CONTROLLED STUDY; ENZYME DEFICIENCY; ESCHERICHIA COLI; GENE DELETION; GENE OVEREXPRESSION; GLYCOLYSIS; NONHUMAN; NUCLEOTIDE METABOLISM; OXIDATION; PENTOSE PHOSPHATE CYCLE; REDUCTION;

BASE SEQUENCE; BIOLOGICAL AVAILABILITY; DNA PRIMERS; ESCHERICHIA COLI; FERMENTATION; NADP; PHOSPHOFRUCTOKINASES; POLYMERASE CHAIN REACTION;

ESCHERICHIA COLI;

EID: 84880510233     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-4859-0     Document Type: Article

References (23)

1. Alper H, Jin YS, Moxley JF, Stephanopoulos G (2005a) Identifying gene targets for the metabolic engineering of lycopene biosynthesis in Escherichia coli. Metab Eng 7(3):155-164. doi: 10.1016/j.ymben.2004.12.003
2. Alper H, Miyaoku K, Stephanopoulos G (2005b) Construction of lycopene-overproducing E. coli strains by combining systematic and combinatorial gene knockout targets. Nat Biotechnol 23(5):612-616. doi: 10.1038/nbt1083
3. Bartek T, Blombach B, Zonnchen E, Makus P, Lang S, Eikmanns BJ, Oldiges M (2010) Importance of NADPH supply for improved L-valine formation in Corynebacterium glutamicum. Biotechnol Prog 26(2):361-371. doi: 10.1002/btpr.345
4. Chemler JA, Fowler ZL, McHugh KP, Koffas MA (2010) Improving NADPH availability for natural product biosynthesis in Escherichia coli by metabolic engineering. Metab Eng 12(2):96-104. doi: 10.1016/j.ymben.2009.07.003
5. Chin JW, Cirino PC (2011) Improved NADPH supply for xylitol production by engineered Escherichia coli with glycolytic mutations. Biotechnol Prog 27(2):333-341. doi: 10.1002/btpr.559
6. Chin JW, Khankal R, Monroe CA, Maranas CD, Cirino PC (2009) Analysis of NADPH supply during xylitol production by engineered Escherichia coli. Biotechnol Bioeng 102(1):209-220. doi: 10.1002/bit.22060
7. Cunningham FX, Jr., Sun Z, Chamovitz D, Hirschberg J, Gantt E (1994) Molecular structure and enzymatic function of lycopene cyclase from the cyanobacterium Synechococcus sp strain PCC7942. Plant Cell 6(8):1107-1121. doi: 10.1105/tpc.6.8.1107
8. Farmer WR, Liao JC (2001) Precursor balancing for metabolic engineering of lycopene production in Escherichia coli. Biotechnol Prog 17(1):57-61. doi: 10.1021/bp000137t
9. Kim S, Lee CH, Nam SW, Kim P (2011a) Alteration of reducing powers in an isogenic phosphoglucose isomerase (pgi)-disrupted Escherichia coli expressing NAD(P)-dependent malic enzymes and NADP-dependent glyceraldehyde 3-phosphate dehydrogenase. Lett Appl Microbiol 52(5):433-440. doi: 10.1111/j.1472-765X.2011. 03013.x
10. Kim YM, Cho HS, Jung GY, Park JM (2011b) Engineering the pentose phosphate pathway to improve hydrogen yield in recombinant Escherichia coli. Biotechnol Bioeng 108(12):2941-2946. doi: 10.1002/bit.23259
11. Kurata A, Kurihara T, Kamachi H, Esaki N (2005) 2-Haloacrylate reductase, a novel enzyme of the medium chain dehydrogenase/reductase superfamily that catalyzes the reduction of a carbon-carbon double bond of unsaturated organohalogen compounds. J Biol Chem 280(21):20286-20291. doi: 10.1074/jbc.M414605200
12. Kurata A, Fujita M, Mowafy AM, Kamachi H, Kurihara T, Esaki N (2008) Production of (S)-2-chloropropionate by asymmetric reduction of 2-chloroacrylate with 2-haloacrylate reductase coupled with glucose dehydrogenase. J Biosci Bioeng 105(4):429-431. doi: 10.1263/jbb.105.429
13. Lee WH, Park JB, Park K, Kim MD, Seo JH (2007) Enhanced production of epsilon-caprolactone by overexpression of NADPH-regenerating glucose 6-phosphate dehydrogenase in recombinant Escherichia coli harboring cyclohexanone monooxygenase gene. Appl Microbiol Biotechnol 76(2):329-338. doi: 10.1007/s00253-007-1016-7
14. Lovingshimer MR, Siegele D, Reinhart GD (2006) Construction of an inducible, pfkA and pfkB deficient strain of Escherichia coli for the expression and purification of phosphofructokinase from bacterial sources. Protein Expr Purif 46(2):475-482. doi: 10.1016/j.pep.2005.09.015
15. Martinez I, Zhu J, Lin H, Bennett GN, San KY (2008) Replacing Escherichia coli NAD-dependent glyceraldehyde 3-phosphate dehydrogenase (GAPDH) with a NADP-dependent enzyme from Clostridium acetobutylicum facilitates NADPH dependent pathways. Metab Eng 10(6):352-359. doi: 10.1016/j.ymben.2008.09.001
16. Phillips GJ, Park SK, Huber D (2000) High copy number plasmids compatible with commonly used cloning vectors. Biotechniques 28(3):400-402, 404, 406 passim
17. Sanchez AM, Andrews J, Hussein I, Bennett GN, San KY (2006) Effect of overexpression of a soluble pyridine nucleotide transhydrogenase (UdhA) on the production of poly(3-hydroxybutyrate) in Escherichia coli. Biotechnol Prog 22(2):420-425. doi: 10.1021/bp050375u
18. Siedler S, Bringer S, Bott M (2011) Increased NADPH availability in Escherichia coli: improvement of the product per glucose ratio in reductive whole-cell biotransformation. Appl Microbiol Biotechnol 92(5):929-937. doi: 10.1007/s00253-011-3374-4
19. Siedler S, Bringer S, Blank LM, Bott M (2012) Engineering yield and rate of reductive biotransformation in Escherichia coli by partial cyclization of the pentose phosphate pathway and PTS-independent glucose transport. Appl Microbiol Biotechnol 93(4):1459-1467. doi: 10.1007/s00253-011-3626-3
20. Vinopal RT, Clifton D, Fraenkel DG (1975) PfkA locus of Escherichia coli. J Bacteriol 122(3):1162-1171
21. Wakao S, Benning C (2005) Genome-wide analysis of glucose-6-phosphate dehydrogenases in Arabidopsis. Plant J 41(2):243-256. doi: 10.1111/j.1365-313X. 2004.02293.x
22. Walton AZ, Stewart JD (2004) Understanding and improving NADPH-dependent reactions by nongrowing Escherichia coli cells. Biotechnol Prog 20(2):403-411. doi: 10.1021/bp030044m
23. Yoon KW, Doo EH, Kim SW, Park JB (2008) In situ recovery of lycopene during biosynthesis with recombinant Escherichia coli. J Biotechnol 135(3):291-294. doi: 10.1016/j.jbiotec.2008.04.001