메뉴 건너뛰기




Volumn 14, Issue 8, 2013, Pages 886-894

Cryo-immunoelectron microscopy of adherent cells improved by the use of electrospun cell culture substrates

Author keywords

Cell culture; Cryofixation; Cryosections; Electrospinning; Freeze substitution; Freeze fracture replica labelling; Gelatin nanofibres; High pressure freezing; Immunogold

Indexed keywords

GELATIN; NANOFIBER;

EID: 84880131235     PISSN: 13989219     EISSN: 16000854     Source Type: Journal    
DOI: 10.1111/tra.12080     Document Type: Article
Times cited : (8)

References (49)
  • 1
    • 0028828220 scopus 로고
    • Freeze-fracture replica electron microscopy combined with SDS digestion for cytochemical labeling of integral membrane proteins. Application to the immunogold labeling of intercellular junctional complexes
    • Fujimoto K. Freeze-fracture replica electron microscopy combined with SDS digestion for cytochemical labeling of integral membrane proteins. Application to the immunogold labeling of intercellular junctional complexes. J Cell Sci 1995;108(Pt 11):3443-3449.
    • (1995) J Cell Sci , vol.108 , Issue.PART 11 , pp. 3443-3449
    • Fujimoto, K.1
  • 3
    • 38949109407 scopus 로고    scopus 로고
    • Cryo-section immunolabelling of difficult to preserve specimens: advantages of cryofixation, freeze-substitution and rehydration
    • Ripper D, Schwarz H, Stierhof YD. Cryo-section immunolabelling of difficult to preserve specimens: advantages of cryofixation, freeze-substitution and rehydration. Biol Cell Eur Cell Biol Organ 2008;100:109-123.
    • (2008) Biol Cell Eur Cell Biol Organ , vol.100 , pp. 109-123
    • Ripper, D.1    Schwarz, H.2    Stierhof, Y.D.3
  • 4
    • 0015620349 scopus 로고
    • A technique for ultracryotomy of cell suspensions and tissues
    • Tokuyasu KT. A technique for ultracryotomy of cell suspensions and tissues. J Cell Biol 1973;57:551-565.
    • (1973) J Cell Biol , vol.57 , pp. 551-565
    • Tokuyasu, K.T.1
  • 8
    • 0036125418 scopus 로고    scopus 로고
    • Relative labelling index: a novel stereological approach to test for non-random immunogold labelling of organelles and membranes on transmission electron microscopy thin sections
    • Mayhew TM, Lucocq JM, Griffiths G. Relative labelling index: a novel stereological approach to test for non-random immunogold labelling of organelles and membranes on transmission electron microscopy thin sections. J Microsc 2002;205(Pt 2):153-164.
    • (2002) J Microsc , vol.205 , Issue.PART 2 , pp. 153-164
    • Mayhew, T.M.1    Lucocq, J.M.2    Griffiths, G.3
  • 9
    • 0032952188 scopus 로고    scopus 로고
    • Direct immunogold labeling of connexins and aquaporin-4 in freeze-fracture replicas of liver, brain, and spinal cord: factors limiting quantitative analysis
    • Rash JE, Yasumura T. Direct immunogold labeling of connexins and aquaporin-4 in freeze-fracture replicas of liver, brain, and spinal cord: factors limiting quantitative analysis. Cell Tissue Res 1999;296:307-321.
    • (1999) Cell Tissue Res , vol.296 , pp. 307-321
    • Rash, J.E.1    Yasumura, T.2
  • 10
    • 84861583671 scopus 로고    scopus 로고
    • Topography of lipid droplet-associated proteins: insights from freeze-fracture replica immunogold labeling
    • Robenek H, Buers I, Robenek MJ, Hofnagel O, Ruebel A, Troyer D, Severs NJ. Topography of lipid droplet-associated proteins: insights from freeze-fracture replica immunogold labeling. J Lipids 2011;2011:409371.
    • (2011) J Lipids , vol.2011 , pp. 409371
    • Robenek, H.1    Buers, I.2    Robenek, M.J.3    Hofnagel, O.4    Ruebel, A.5    Troyer, D.6    Severs, N.J.7
  • 11
    • 0031052242 scopus 로고    scopus 로고
    • A simple and reliable quick-freezing/freeze-fracturing procedure
    • Fujimoto K, Noda T, Fujimoto T. A simple and reliable quick-freezing/freeze-fracturing procedure. Histochem Cell Biol 1997;107:81-84.
    • (1997) Histochem Cell Biol , vol.107 , pp. 81-84
    • Fujimoto, K.1    Noda, T.2    Fujimoto, T.3
  • 12
    • 84871204420 scopus 로고    scopus 로고
    • A method for efficient observation of intracellular membranes of monolayer culture cells by quick-freeze and freeze-fracture electron microscopy
    • Fujita A, Fujimoto T, Ozato-Sakurai N, Suzuki H. A method for efficient observation of intracellular membranes of monolayer culture cells by quick-freeze and freeze-fracture electron microscopy. J Electron Microsc 2012;61:441-446.
    • (2012) J Electron Microsc , vol.61 , pp. 441-446
    • Fujita, A.1    Fujimoto, T.2    Ozato-Sakurai, N.3    Suzuki, H.4
  • 13
    • 23744484015 scopus 로고    scopus 로고
    • Belt-like localisation of caveolin in deep caveolae and its re-distribution after cholesterol depletion
    • Westermann M, Steiniger F, Richter W. Belt-like localisation of caveolin in deep caveolae and its re-distribution after cholesterol depletion. Histochem Cell Biol 2005;123:613-620.
    • (2005) Histochem Cell Biol , vol.123 , pp. 613-620
    • Westermann, M.1    Steiniger, F.2    Richter, W.3
  • 14
    • 0025802101 scopus 로고
    • Adult rat cardiomyocytes cultured in creatine-deficient medium display large mitochondria with paracrystalline inclusions, enriched for creatine kinase
    • Eppenberger-Eberhardt M, Riesinger I, Messerli M, Schwarb P, Muller M, Eppenberger HM, Wallimann T. Adult rat cardiomyocytes cultured in creatine-deficient medium display large mitochondria with paracrystalline inclusions, enriched for creatine kinase. J Cell Biol 1991;113:289-302.
    • (1991) J Cell Biol , vol.113 , pp. 289-302
    • Eppenberger-Eberhardt, M.1    Riesinger, I.2    Messerli, M.3    Schwarb, P.4    Muller, M.5    Eppenberger, H.M.6    Wallimann, T.7
  • 15
    • 0343526767 scopus 로고    scopus 로고
    • Cryopreparation provides new insight into the effects of brefeldin A on the structure of the HepG2 Golgi apparatus
    • Hess MW, Muller M, Debbage PL, Vetterlein M, Pavelka M. Cryopreparation provides new insight into the effects of brefeldin A on the structure of the HepG2 Golgi apparatus. J Struct Biol 2000;130:63-72.
    • (2000) J Struct Biol , vol.130 , pp. 63-72
    • Hess, M.W.1    Muller, M.2    Debbage, P.L.3    Vetterlein, M.4    Pavelka, M.5
  • 16
    • 0142025255 scopus 로고    scopus 로고
    • Direct attachment of cell suspensions to high-pressure freezing specimen planchettes
    • Sawaguchi A, Yao X, Forte JG, McDonald KL. Direct attachment of cell suspensions to high-pressure freezing specimen planchettes. J Microsc 2003;212(Pt 1):13-20.
    • (2003) J Microsc , vol.212 , Issue.PART 1 , pp. 13-20
    • Sawaguchi, A.1    Yao, X.2    Forte, J.G.3    McDonald, K.L.4
  • 17
    • 56149101340 scopus 로고    scopus 로고
    • Correlative light-electron microscopy (CLEM) combining live-cell imaging and immunolabeling of ultrathin cryosections
    • van Rijnsoever C, Oorschot V, Klumperman J. Correlative light-electron microscopy (CLEM) combining live-cell imaging and immunolabeling of ultrathin cryosections. Nat Methods 2008;5:973-980.
    • (2008) Nat Methods , vol.5 , pp. 973-980
    • van Rijnsoever, C.1    Oorschot, V.2    Klumperman, J.3
  • 18
  • 19
    • 34547475023 scopus 로고    scopus 로고
    • Electrospinning: a fascinating method for the preparation of ultrathin fibers
    • Greiner A, Wendorff JH. Electrospinning: a fascinating method for the preparation of ultrathin fibers. Angew Chem Int Ed 2007;46:5670-5703.
    • (2007) Angew Chem Int Ed , vol.46 , pp. 5670-5703
    • Greiner, A.1    Wendorff, J.H.2
  • 21
    • 84894408785 scopus 로고    scopus 로고
    • Spinning tale. Electrospinning: mature technology being used in new ways
    • Gillespie LK. Spinning tale. Electrospinning: mature technology being used in new ways. MicroManufacturing 2011;4:38-45.
    • (2011) MicroManufacturing , vol.4 , pp. 38-45
    • Gillespie, L.K.1
  • 22
    • 80355132955 scopus 로고    scopus 로고
    • Electrospinningfundamentals: optimizing solution and apparatus parameters
    • doi:2410.3791/2494.
    • Leach MK, Feng ZQ, Tuck SJ, Corey JM. Electrospinningfundamentals: optimizing solution and apparatus parameters. J Vis Exp 2011;47:e2494, doi:2410.3791/2494.
    • (2011) J Vis Exp , vol.47
    • Leach, M.K.1    Feng, Z.Q.2    Tuck, S.J.3    Corey, J.M.4
  • 23
    • 3042677215 scopus 로고    scopus 로고
    • Electrospinning and mechanical characterization of gelatin nanofibers
    • Huang Z-M, Zhang YZ, Ramakrishna S, Lim CT. Electrospinning and mechanical characterization of gelatin nanofibers. Polymer 2004;45:5361-5368.
    • (2004) Polymer , vol.45 , pp. 5361-5368
    • Huang, Z.-M.1    Zhang, Y.Z.2    Ramakrishna, S.3    Lim, C.T.4
  • 24
    • 34147094231 scopus 로고    scopus 로고
    • Guidance of glial cell migration and axonal growth on electrospun nanofibers of poly-epsilon-caprolactone and a collagen/poly-epsilon-caprolactone blend
    • Schnell E, Klinkhammer K, Balzer S, Brook G, Klee D, Dalton P, Mey J. Guidance of glial cell migration and axonal growth on electrospun nanofibers of poly-epsilon-caprolactone and a collagen/poly-epsilon-caprolactone blend. Biomaterials 2007;28:3012-3025.
    • (2007) Biomaterials , vol.28 , pp. 3012-3025
    • Schnell, E.1    Klinkhammer, K.2    Balzer, S.3    Brook, G.4    Klee, D.5    Dalton, P.6    Mey, J.7
  • 25
    • 79959244198 scopus 로고    scopus 로고
    • Electrospun collagen: a tissue engineering scaffold with unique functional properties in a wide variety of applications
    • Jha BS, Ayres CE, Bowman JR, Telemeco TA, Sell SA, Bowlin GL, Simpson DG. Electrospun collagen: a tissue engineering scaffold with unique functional properties in a wide variety of applications. J Nanomater 2011:348268.
    • (2011) J Nanomater , pp. 348268
    • Jha, B.S.1    Ayres, C.E.2    Bowman, J.R.3    Telemeco, T.A.4    Sell, S.A.5    Bowlin, G.L.6    Simpson, D.G.7
  • 26
    • 84858684854 scopus 로고    scopus 로고
    • Viability, proliferation and adhesion of smooth muscle cells and human umbilical vein endothelial cells on electrospun polymer scaffolds
    • Ruder C, Sauter T, Becker T, Kratz K, Hiebl B, Jung F, Lendlein A, Zohlnhofer D. Viability, proliferation and adhesion of smooth muscle cells and human umbilical vein endothelial cells on electrospun polymer scaffolds. Clin Hemorheol Microcirc 2012;50:101-112.
    • (2012) Clin Hemorheol Microcirc , vol.50 , pp. 101-112
    • Ruder, C.1    Sauter, T.2    Becker, T.3    Kratz, K.4    Hiebl, B.5    Jung, F.6    Lendlein, A.7    Zohlnhofer, D.8
  • 28
    • 84863075981 scopus 로고    scopus 로고
    • MG63 osteoblast-like cells exhibit different behavior when grown on electrospun collagen matrix versus electrospun gelatin matrix
    • Tsai SW, Liou HM, Lin CJ, Kuo KL, Hung YS, Weng RC, Hsu FY. MG63 osteoblast-like cells exhibit different behavior when grown on electrospun collagen matrix versus electrospun gelatin matrix. PLoS One 2012;7:e31200.
    • (2012) PLoS One , vol.7
    • Tsai, S.W.1    Liou, H.M.2    Lin, C.J.3    Kuo, K.L.4    Hung, Y.S.5    Weng, R.C.6    Hsu, F.Y.7
  • 29
    • 0019196784 scopus 로고
    • Fibronectin is not present in the focal adhesions formed between normal cultured fibroblasts and their substrata
    • Chen WT, Singer SJ. Fibronectin is not present in the focal adhesions formed between normal cultured fibroblasts and their substrata. Proc Natl Acad Sci USA 1980;77:7318-7322.
    • (1980) Proc Natl Acad Sci USA , vol.77 , pp. 7318-7322
    • Chen, W.T.1    Singer, S.J.2
  • 31
    • 0034896860 scopus 로고    scopus 로고
    • Thermo-responsive culture dishes allow the intact harvest of multilayered keratinocyte sheets without dispase by reducing temperature
    • Yamato M, Utsumi M, Kushida A, Konno C, Kikuchi A, Okano T. Thermo-responsive culture dishes allow the intact harvest of multilayered keratinocyte sheets without dispase by reducing temperature. Tissue Eng 2001;7:473-480.
    • (2001) Tissue Eng , vol.7 , pp. 473-480
    • Yamato, M.1    Utsumi, M.2    Kushida, A.3    Konno, C.4    Kikuchi, A.5    Okano, T.6
  • 33
    • 0025910689 scopus 로고
    • Rapid-freezing cytochemistry: preservation of tubular lysosomes and enzyme activity
    • Robinson JM, Karnovsky MJ. Rapid-freezing cytochemistry: preservation of tubular lysosomes and enzyme activity. J Histochem Cytochem 1991;39:787-792.
    • (1991) J Histochem Cytochem , vol.39 , pp. 787-792
    • Robinson, J.M.1    Karnovsky, M.J.2
  • 34
    • 0002268153 scopus 로고
    • Cryofixation of thick specimens by high-pressure freezing
    • Haggins GH, editor. Chicago: SEM Inc. AMF O'Hare
    • Müller M, Moor H. Cryofixation of thick specimens by high-pressure freezing. In: Haggins GH, editor. The Science of Biological Specimen Preparation. Chicago: SEM Inc. AMF O'Hare; 1984, pp. 131-138.
    • (1984) The Science of Biological Specimen Preparation , pp. 131-138
    • Müller, M.1    Moor, H.2
  • 35
  • 36
    • 0029762913 scopus 로고    scopus 로고
    • Improving structural integrity of cryosections for immunogold labeling
    • Liou W, Geuze HJ, Slot JW. Improving structural integrity of cryosections for immunogold labeling. Histochem Cell Biol 1996;106:41-58.
    • (1996) Histochem Cell Biol , vol.106 , pp. 41-58
    • Liou, W.1    Geuze, H.J.2    Slot, J.W.3
  • 37
    • 79956206895 scopus 로고    scopus 로고
    • A new approach to improve the quality of ultrathin cryo-sections; its use for immunogold EM and correlative electron cryo-tomography
    • Bos E, SantAnna C, Gnaegi H, Pinto RF, Ravelli RB, Koster AJ, de Souza W, Peters PJ. A new approach to improve the quality of ultrathin cryo-sections; its use for immunogold EM and correlative electron cryo-tomography. J Struct Biol 2011;175:62-72.
    • (2011) J Struct Biol , vol.175 , pp. 62-72
    • Bos, E.1    SantAnna, C.2    Gnaegi, H.3    Pinto, R.F.4    Ravelli, R.B.5    Koster, A.J.6    de Souza, W.7    Peters, P.J.8
  • 38
    • 74449094003 scopus 로고    scopus 로고
    • Improving the technique of vitreous cryo-sectioning for cryo-electron tomography: electrostatic charging for section attachment and implementation of an anti-contamination glove box
    • Pierson J, Fernandez JJ, Bos E, Amini S, Gnaegi H, Vos M, Bel B, Adolfsen F, Carrascosa JL, Peters PJ. Improving the technique of vitreous cryo-sectioning for cryo-electron tomography: electrostatic charging for section attachment and implementation of an anti-contamination glove box. J Struct Biol 2010;169:219-225.
    • (2010) J Struct Biol , vol.169 , pp. 219-225
    • Pierson, J.1    Fernandez, J.J.2    Bos, E.3    Amini, S.4    Gnaegi, H.5    Vos, M.6    Bel, B.7    Adolfsen, F.8    Carrascosa, J.L.9    Peters, P.J.10
  • 39
    • 0036143405 scopus 로고    scopus 로고
    • A reliable and convenient method to store ultrathin thawed cryosections prior to immunolabeling
    • Griffith JM, Posthuma G. A reliable and convenient method to store ultrathin thawed cryosections prior to immunolabeling. J Histochem Cytochem 2002;50:57-62.
    • (2002) J Histochem Cytochem , vol.50 , pp. 57-62
    • Griffith, J.M.1    Posthuma, G.2
  • 41
    • 40649100773 scopus 로고    scopus 로고
    • Electron tomography of vitreous sections from cultured mammalian cells
    • Gruska M, Medalia O, Baumeister W, Leis A. Electron tomography of vitreous sections from cultured mammalian cells. J Struct Biol 2008;161:384-392.
    • (2008) J Struct Biol , vol.161 , pp. 384-392
    • Gruska, M.1    Medalia, O.2    Baumeister, W.3    Leis, A.4
  • 43
    • 0027283602 scopus 로고
    • An in vitro model for the analysis of intestinal brush border assembly. I. Ultrastructural analysis of cell contact-induced brush border assembly in Caco-2BBe cells
    • Peterson MD, Mooseker MS. An in vitro model for the analysis of intestinal brush border assembly. I. Ultrastructural analysis of cell contact-induced brush border assembly in Caco-2BBe cells. J Cell Sci 1993;105(Pt 2):445-460.
    • (1993) J Cell Sci , vol.105 , Issue.PART 2 , pp. 445-460
    • Peterson, M.D.1    Mooseker, M.S.2
  • 44
    • 53849117085 scopus 로고    scopus 로고
    • Loss of the Batten disease gene CLN3 prevents exit from the TGN of the mannose 6-phosphate receptor
    • Metcalf DJ, Calvi AA, Seaman M, Mitchison HM, Cutler DF. Loss of the Batten disease gene CLN3 prevents exit from the TGN of the mannose 6-phosphate receptor. Traffic 2008;9:1905-1914.
    • (2008) Traffic , vol.9 , pp. 1905-1914
    • Metcalf, D.J.1    Calvi, A.A.2    Seaman, M.3    Mitchison, H.M.4    Cutler, D.F.5
  • 45
    • 0023890132 scopus 로고
    • Ultrathin cryosections in the plane of cell monolayers: evaluation of their potential for antibody localization studies of the cytoskeleton
    • Langanger G, De Mey J. Ultrathin cryosections in the plane of cell monolayers: evaluation of their potential for antibody localization studies of the cytoskeleton. J Electron Microsc Tech 1988;8:391-399.
    • (1988) J Electron Microsc Tech , vol.8 , pp. 391-399
    • Langanger, G.1    De Mey, J.2
  • 47
    • 0036329615 scopus 로고    scopus 로고
    • A novel flat-embedding method to prepare ultrathin cryosections from cultured cells in their in situ orientation
    • Oorschot V, de Wit H, Annaert WG, Klumperman J. A novel flat-embedding method to prepare ultrathin cryosections from cultured cells in their in situ orientation. J Histochem Cytochem 2002;50:1067-1080.
    • (2002) J Histochem Cytochem , vol.50 , pp. 1067-1080
    • Oorschot, V.1    de Wit, H.2    Annaert, W.G.3    Klumperman, J.4
  • 48
    • 33847208808 scopus 로고    scopus 로고
    • Number and density of AMPA receptors in individual synapses in the rat cerebellum as revealed by SDS-digested freeze-fracture replica labeling
    • Masugi-Tokita M, Tarusawa E, Watanabe M, Molnar E, Fujimoto K, Shigemoto R. Number and density of AMPA receptors in individual synapses in the rat cerebellum as revealed by SDS-digested freeze-fracture replica labeling. J Neurosci 2007;27:2135-2144.
    • (2007) J Neurosci , vol.27 , pp. 2135-2144
    • Masugi-Tokita, M.1    Tarusawa, E.2    Watanabe, M.3    Molnar, E.4    Fujimoto, K.5    Shigemoto, R.6
  • 49
    • 77955276500 scopus 로고    scopus 로고
    • Two distinct pools of large-conductance calcium-activated potassium channels in the somatic plasma membrane of central principal neurons
    • Kaufmann WA, Kasugai Y, Ferraguti F, Storm JF. Two distinct pools of large-conductance calcium-activated potassium channels in the somatic plasma membrane of central principal neurons. Neuroscience 2010;169:974-986.
    • (2010) Neuroscience , vol.169 , pp. 974-986
    • Kaufmann, W.A.1    Kasugai, Y.2    Ferraguti, F.3    Storm, J.F.4


* 이 정보는 Elsevier사의 SCOPUS DB에서 KISTI가 분석하여 추출한 것입니다.