A simple and reliable quick-freezing/freeze-fracturing procedure

Histochemistry and Cell Biology

Volumn 107, Issue 1, 1997, Pages 81-84

  Fujimoto, Kazushi ^a   Noda, Toru ^a   Fujimoto, Toyoshi ^a,b  

^a KYOTO UNIVERSITY   (Japan)

^b GUNMA UNIVERSITY   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL CELL; ARTICLE; CELL CULTURE; DOG; ELECTRON MICROSCOPY; FREEZE FRACTURE; NONHUMAN; PRIORITY JOURNAL; TECHNIQUE;

ANIMALS; CELLS, CULTURED; COPPER; DOGS; FIXATIVES; FREEZE FRACTURING; FREEZING; HELIUM; KIDNEY; NA(+)-K(+)-EXCHANGING ATPASE; RATS; TISSUE FIXATION;

ANIMALIA; CANIS FAMILIARIS;

EID: 0031052242     PISSN: 09486143     EISSN: None     Source Type: Journal    
DOI: 10.1007/s004180050091     Document Type: Article

References (6)

1. Fisher KA (1975) Half membrane enrichment: verification by electron microscopy. Science 190:983-984
2. Fujimoto K (1995) Freeze-fracture replica electron microscopy combined with SDS digestion for cytochemical labeling of integral membrane proteins. Application to the immunogold labeling of intercellular junctional complexes. J Cell Sci 108:3443-3449
3. Fujimoto K, Moller JV, Maunsbach AB (1996a) Epitope topology of Na, K-ATPase α subunit analyzed in basolateral cell membranes of rat kidney tubules. FEBS Lett 395:29-32
4. Fujimoto K, Umeda M, Fujimoto T (1996b) Transmembrane phospholipid distribution revealed by freeze-fracture replica labeling. J Cell Sci 109:2453-2460
5. Furuse M, Fujimoto K, Sato N, Hirase T, Tsukita Sa, Tsukita Sh (1996) Overexpression of occluding, a tight junction-associated integral membrane protein, induces the formation of intracellular multilamellar bodies bearing tight junction-like structures. J Cell Sci 109:429-435
6. Heuser JE, Reese TS, Jan LY, Dennis MJ, Evans L (1979) Synaptic vesicle exocytosis captured by quick-freezing and correlated with qnantal transmitter release. J Cell Biol 81:275-300