Tagging of Genomic STAT3 and STAT1 with Fluorescent Proteins and Insertion of a Luciferase Reporter in the Cyclin D1 Gene Provides a Modified A549 Cell Line to Screen for Selective STAT3 Inhibitors

PLoS ONE

Volumn 8, Issue 7, 2013, Pages

  Samsonov, Andrey ^a   Zenser, Nathan ^a   Zhang, Fan ^a   Zhang, Hongyi ^a   Fetter, John ^a   Malkov, Dmitry ^a  

^a Cell Based Assays Reporter Cell Lines Sigma Aldrich   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CYCLIN D1; GAMMA INTERFERON; GREEN FLUORESCENT PROTEIN; INTERLEUKIN 6; LUCIFERASE; PROTEIN INHIBITOR; RED FLUORESCENT PROTEIN; STAT1 PROTEIN; STAT3 PROTEIN; STAT3 PROTEIN INHIBITOR; UNCLASSIFIED DRUG; ZINC FINGER NUCLEASE;

AMINO TERMINAL SEQUENCE; ARTICLE; CANCER CELL CULTURE; CARBOXY TERMINAL SEQUENCE; CARCINOMA CELL; CELL CYCLE PROGRESSION; CELL GROWTH; CELL STRAIN A549; CONTROLLED STUDY; CYCLIN D1 GENE; GENE LOCUS; GROWTH INHIBITION; HOMOLOGOUS RECOMBINATION; HUMAN; HUMAN CELL; LUNG CARCINOMA; PROMOTER REGION; PROTEIN EXPRESSION; TRANSGENE;

BASE SEQUENCE; CELL LINE, TUMOR; CYCLIN D1; DRUG EVALUATION, PRECLINICAL; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HUMANS; LUCIFERASES; LUMINESCENT PROTEINS; MOLECULAR SEQUENCE DATA; MUTAGENESIS, INSERTIONAL; PROTEIN ENGINEERING; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; STAT1 TRANSCRIPTION FACTOR; STAT3 TRANSCRIPTION FACTOR;

EID: 84879960450     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0068391     Document Type: Article

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