Crystal structures of the ternary complex of APH(4)-Ia/Hph with hygromycin B and an ATP analog using a thermostable mutant

Journal of Structural Biology

Volumn 183, Issue 1, 2013, Pages 76-85

  Iino, Daisuke ^a   Takakura, Yasuaki ^b   Fukano, Kazuhiro ^a   Sasaki, Yasuyuki ^a   Hoshino, Takayuki ^b   Ohsawa, Kanju ^a   Nakamura, Akira ^b   Yajima, Shunsuke ^a  

^a TOKYO UNIVERSITY OF AGRICULTURE   (Japan)

^b UNIVERSITY OF TSUKUBA   (Japan)

Author keywords

Aminoglycoside antibiotics; Conformational change; Crystal structure; Kinase; Thermostability

Indexed keywords

ADENOSINE TRIPHOSPHATE DERIVATIVE; AMINOGLYCOSIDE 4 PHOSPHOTRANSFERASE IA; AMP PNP; HYGROMYCIN B; HYGROMYCIN B PHOSPHOTRANSFERASE; HYGROMYCIN B PHOSPHOTRANSFERASE 5; PHOSPHOTRANSFERASE; UNCLASSIFIED DRUG;

AMINO ACID SUBSTITUTION; ARTICLE; CATALYSIS; CRYSTAL STRUCTURE; ENZYME SPECIFICITY; ENZYME SUBSTRATE; ENZYME SUBSTRATE COMPLEX; HYDROPHOBICITY; MUTATION; NUCLEOTIDE BINDING SITE; PRIORITY JOURNAL; PROTEIN BINDING; PROTEIN DOMAIN; THERMOSTABILITY;

AMINOGLYCOSIDE ANTIBIOTICS; CONFORMATIONAL CHANGE; CRYSTAL STRUCTURE; KINASE; THERMOSTABILITY;

BINDING SITES; CRYSTALLOGRAPHY; ESCHERICHIA COLI; HYGROMYCIN B; KANAMYCIN KINASE; MODELS, MOLECULAR; MUTAGENESIS, SITE-DIRECTED; PHOSPHORYLATION; PHOSPHOTRANSFERASES (ALCOHOL GROUP ACCEPTOR); PROTEIN STABILITY; TEMPERATURE;

EID: 84879884913     PISSN: 10478477     EISSN: 10958657     Source Type: Journal    
DOI: 10.1016/j.jsb.2013.05.023     Document Type: Article

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