Improvement of gougerotin and nikkomycin production by engineering their biosynthetic gene clusters

Applied Microbiology and Biotechnology

Volumn 97, Issue 14, 2013, Pages 6383-6396

  Du, Deyao ^a,b   Zhu, Yu ^a,b   Wei, Junhong ^a,b   Tian, Yuqing ^a   Niu, Guoqing ^a   Tan, Huarong ^a  

^a INSTITUTE OF MICROBIOLOGY   (China)

^b UNIVERSITY OF CHINESE ACADEMY OF SCIENCES   (China)

Author keywords

Engineering; Gougerotin; Nikkomycin; Promoter activity; Streptomyces

Indexed keywords

BIOSYNTHETIC GENE CLUSTER; GOUGEROTIN; MAJOR FACILITATOR SUPERFAMILY; NIKKOMYCIN; PROMOTER ACTIVITIES; STREPTOMYCES; STREPTOMYCES ANSOCHROMOGENES; STREPTOMYCES COELICOLOR;

ANTIBIOTICS; BACTERIA; BIOCHEMISTRY; BIOSYNTHESIS; ENGINEERING;

GENES;

ANTIBIOTIC AGENT; GOUGEROTIN; NIKKOMYCIN; UNCLASSIFIED DRUG;

ANTIBIOTICS; BACTERIUM; BIOLOGICAL ANALYSIS; GENE; GENETIC ENGINEERING; PEPTIDE;

ANTIBIOTIC BIOSYNTHESIS; ARTICLE; CONTROLLED STUDY; GENE CLUSTER; GENETIC ENGINEERING; GENETIC MANIPULATION; NONHUMAN; PROMOTER REGION; STREPTOMYCES; STREPTOMYCES ANSOCHROMOGENES; STREPTOMYCES COELICOLOR;

ANTI-BACTERIAL AGENTS; BACTERIAL PROTEINS; GENE EXPRESSION REGULATION, BACTERIAL; GENETIC ENGINEERING; MULTIGENE FAMILY; PROMOTER REGIONS, GENETIC; PYRIMIDINE NUCLEOSIDES; STREPTOMYCES;

STREPTOMYCES; STREPTOMYCES ANSOCHROMOGENES; STREPTOMYCES COELICOLOR;

EID: 84879843040     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-4836-7     Document Type: Article

References (33)

1. Bibb MJ, Janssen GR, Ward JM (1985) Cloning and analysis of the promoter region of the erythromycin resistance gene (ermE) of Streptomyces erythraeus. Gene 38:215-226
2. Clark JM, Gunther JK (1963) Gougerotin, a specific inhibitor of protein synthesis. Biochim Biophys Acta 76:636-638
3. Dangel V, Westrich L, Smith M, Heide L, Gust B (2010) Use of an inducible promoter for antibiotic production in a heterologous host. Appl Microbiol Biotechnol 87:261-269
4. Datsenko KA, Wanner BL (2000) One-step inactivation of chromosomal genes in Escherichia coli K-12 using PCR products. Proc Natl Acad Sci U S A 97:6640-6645
5. Denis F, Brzezinski R (1991) An improved aminoglycoside resistance gene cassette for use in Gram-negative bacteria and Streptomyces. FEMS Microbiol Lett 81:261-264
6. Fiedler HP (1984) Screening for new microbial products by high-performance liquid chromatography using a photodiode array detector. J Chromatogr 316:487-494
7. Fiedler HP, Kurth R, Langhärig J, Delzer J, Zähner H (1982) Nikkomycins: microbial inhibitors of chitin synthase. J Chem Technol Biotechnol 32:271-280
8. Gomez-Escribano JP, Bibb MJ (2011) Engineering Streptomyces coelicolor for heterologous expression of secondary metabolite gene clusters. Microb Biotechnol 4:207-215
9. Guo J, Zhao J, Li L, Chen Z, Wen Y, Li J (2010) The pathway-specific regulator AveR from Streptomyces avermitilis positively regulates avermectin production while it negatively affects oligomycin biosynthesis. Mol Genet Genomics 283:123-133
10. Haneishi T, Arai M, Kitano N, Yamamoto S (1974) Aspiculamycin, a new cytosine nucleoside antibiotic. 3. Biological activities, in vitro and in vivo. J Antibiot 27:339-342
11. Herai S, Hashimoto Y, Higashibata H, Maseda H, Ikeda H, Omura S, Kobayashi M (2004) Hyper-inducible expression system for Streptomycetes. Proc Natl Acad Sci U S A 101:14031-14035
12. Jung WS, Jeong SJ, Park SR, Choi CY, Park BC, Park JW, Yoon YJ (2008) Enhanced heterologous production of desosaminyl macrolides and their hydroxylated derivatives by overexpression of the pikD regulatory gene in Streptomyces venezuelae. Appl Environ Microbiol 74:1972-1979
13. Kieser T, Bibb MJ, Buttner MJ, Chater KF, Hopwood DA (2000) Practical Streptomyces genetics. The John Innes Foundation, Norwich
14. Kondo F, Kitano N, Domon H, Arai M, Haneishi T (1974) Aspiculamycin, a new cytosine nucleoside antibiotic. IV. Antimycoplasma activity of aspiculamycin in vitro and in vivo. J Antibiot 27:529-534
15. Labes G, Bibb M, Wohlleben W (1997) Isolation and characterization of a strong promoter element from the Streptomyces ghanaensis phage I19 using the gentamicin resistance gene (aacC1) of Tn 1696 as reporter. Microbiology 143:1503-1512
16. Lacal JC, Vázquez D, Fernandez-Sousa JM, Carrasco L (1980) Antibiotics that specifically block translation in virus-infected cells. J Antibiot 33:441-446
17. Li Y, Ling H, Li W, Tan H (2005) Improvement of nikkomycin production by enhanced copy of sanU and sanV in Streptomyces ansochromogenes and characterization of a novel glutamate mutase encoded by sanU and sanV. Metab Eng 7:165-173
18. Li R, Xie Z, Tian Y, Yang H, Chen W, You D, Liu G, Deng Z, Tan H (2009) polR, a pathway-specific transcriptional regulatory gene, positively controls polyoxin biosynthesis in Streptomyces cacaoi subsp. asoensis. Microbiology 155:1819-1831
19. Liao G, Li J, Li L, Yang H, Tian Y, Tan H (2009) Selectively improving nikkomycin Z production by blocking the imidazolone biosynthetic pathway of nikkomycin X and uracil feeding in Streptomyces ansochromogenes. Microb Cell Fact 8:61
20. Liao G, Li J, Li L, Yang H, Tian Y, Tan H (2010) Cloning, reassembling and integration of the entire nikkomycin biosynthetic gene cluster into Streptomyces ansochromogenes lead to an improved nikkomycin production. Microb Cell Fact 9:6
21. Liu G, Tian Y, Yang H, Tan H (2005) A pathway-specific transcriptional regulatory gene for nikkomycin biosynthesis in Streptomyces ansochromogenes that also influences colony development. Mol Microbiol 55:1855-1866
22. Medema MH, Alam MT, Breitling R, Takano E (2011) The future of industrial antibiotic production: from random mutagenesis to synthetic biology. Bioeng Bugs 2:230-233
23. Niu G, Li L, Wei J, Tan H (2013) Cloning, heterologous expression, and characterization of the gene cluster required for gougerotin biosynthesis. Chem Biol 20:34-44
24. E is required for normal cell wall structure in Streptomyces coelicolor A3(2). J Bacteriol 181:204-211
25. Pan Y, Liu G, Yang H, Tian Y, Tan H (2009) The pleiotropic regulator AdpA-L directly controls the pathway-specific activator of nikkomycin biosynthesis in Streptomyces ansochromogenes. Mol Microbiol 72:710-723
26. Rodríguez-García A, Combes P, Pérez-Redondo R, Smith MC, Smith MC (2005) Natural and synthetic tetracycline-inducible promoters for use in the antibiotic-producing bacteria Streptomyces. Nucleic Acids Res 33:e87
27. Sambrook J, Russell DW (2001) Molecular cloning: a laboratory manual. Cold spring harbor laboratory, Cold Spring Harbor
28. Seghezzi N, Amar P, Koebmann B, Jensen PR, Virolle MJ (2011) The construction of a library of synthetic promoters revealed some specific features of strong Streptomyces promoters. Appl Microbiol Biotechnol 90:615-623
29. Takano E, White J, Thompson CJ, Bibb MJ (1995) Construction of thiostrepton-inducible, high-copy-number expression vectors for use in Streptomyces spp. Gene 166:133-137
30. Wang G, Tan H (2004) Enhanced production of nikkomycin X by over-expression of SanO, a non-ribosomal peptide synthetase in Streptomyces ansochromogenes. Biotechnol Lett 26:229-233
31. 2+-mediated regulation of spore germination and aerial hypha formation in Streptomyces coelicolor. J Bacteriol 190:4061-4068
32. Yuan T, Yin C, Zhu C, Zhu B, Hu Y (2011) Improvement of antibiotic productivity by knock-out of dauW in Streptomyces coeruleobidus. Microbiol Res 166:539-547
33. Zeng H, Tan H, Li J (2002) Cloning and function of sanQ: a gene involved in nikkomycin biosynthesis of Streptomyces ansochromogenes. Curr Microbiol 45:175-179