Automated parallel recordings of topologically identified single ion channels

Scientific Reports

Volumn 3, Issue , 2013, Pages

  Kawano, Ryuji ^a   Tsuji, Yutaro ^a,c   Sato, Koji ^b   Osaki, Toshihisa ^a   Kamiya, Koki ^a   Hirano, Minako ^d,e   Ide, Toru ^d,e   Miki, Norihisa ^a,c   Takeuchi, Shoji ^a,b  

^a KANAGAWA ACADEMY OF SCIENCE AND TECHNOLOGY   (Japan)

^b INSTITUTE OF INDUSTRIAL SCIENCE   (Japan)

^c KEIO UNIVERSITY   (Japan)

^d GRADUATE SCHOOL FOR THE CREATION OF NEW PHOTONICS INDUSTRIES   (Japan)

^e RIKEN   (Japan)

Author keywords

[No Author keywords available]

Indexed keywords

LARGE CONDUCTANCE CALCIUM ACTIVATED POTASSIUM CHANNEL; LIPID BILAYER;

AUTOMATION; HUMAN; LIPID BILAYER; PHYSIOLOGY; PROBABILITY; ARTICLE;

AUTOMATION; HUMANS; LARGE-CONDUCTANCE CALCIUM-ACTIVATED POTASSIUM CHANNELS; LIPID BILAYERS; PROBABILITY;

EID: 84879642833     PISSN: None     EISSN: 20452322     Source Type: Journal    
DOI: 10.1038/srep01995     Document Type: Article

References (48)

1. Drews, J. Drug discovery: A historical perspective. Science 287, 1960-1964 (2000). (Pubitemid 30158662)
2. Overington, J. P., Al-Lazikani, B., Hopkins, A. L. Opinion-How many drug targets are there? Nature Reviews Drug Discovery 5, 993-996 (2006). (Pubitemid 44835126)
3. Terstappen, G. C. & Reggiani, A. In silico research in drug discovery. Trends in Pharmacological Sciences 22, 23-26 (2001). (Pubitemid 32126282)
4. Macpherson, L. J. et al. Noxious compounds activate TRPA1 ion channels through covalent modification of cysteines. Nature 445, 541-545 (2007).
5. Rajendran, L., Knoelker, H.-J. & Simons, K. Subcellular targeting strategies for drug design and delivery. Nature Reviews Drug Discovery 9, 29-42 (2010).
6. Hamill, O. P., Marty, A.,Neher, E., Sakmann, B. & Sigworth, F. J. Improved patchclamp techniques for high-resolution current recording from cells and cell-free membrane patches. Pflugers Archiv-European Journal of Physiology 391, 85-100 (1981). (Pubitemid 11012976)
7. Neher, E. Correction for liquid junction potentials in patch clamp experiments. Methods Enzymol. 207, 123-131 (1992).
8. Latorre, R. & Alvarez, O. Voltage-dependent channels in planar lipid bilayer membranes. Physiological Reviews 61, 77-150 (1981).
9. Montal, M. & Mueller, P. Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties. Proc. Natl. Acad. Sci. U.S.A. 69, 3561-3566 (1972).
10. Ide, T. & Yanagida, T. An artificial lipid bilayer formed on an agarose-coated glass for simultaneous electrical and optical measurement of single ion channels. Biochem. Biophys. Res. Commun. 265, 595-599 (1999).
11. Ervin, E., Kawano, R., White, R., White, H. Simultaneous alternating and direct current readout of protein ion channel blocking events using glass nanopore membranes. Anal. Chem. 80, 2069-2076 (2008). (Pubitemid 351458054)
12. Bayley, H. & Martin, C. R. Resistive-pulse sensing-From microbes to molecules. Chem. Rev. 100, 2575-2594 (2000).
13. Heron, A. J., Thompson, J. R., Mason, A. E. & Wallace, M. I. Direct detection of membrane channels from gels using water-in-oil droplet bilayers. J. Am. Chem. Soc. 129, 16042-16047 (2007).
14. Dunlop, J., Bowlby, M., Peri, R., Vasilyev, D. & Arias, R. High-throughput electrophysiology: an emerging paradigm for ion-channel screening and physiology. Nature Reviews Drug Discovery 7, 358-368 (2008).
15. Rotem, D., Mason, A. & Bayley, H. Inactivation of the KcsA potassium channel explored with heterotetramers. J. Gen. Physiol. 135, 29-42 (2010).
16. Syeda, R.,Holden, M. A., Hwang, W. L. & Bayley, H. Screening Blockers Against a Potassium Channel with a Droplet Interface Bilayer Array. J. Am. Chem. Soc. 130, 15543-15548 (2008).
17. El-Arabi, A. M., Salazar, C. S. & Schmidt, J. J. Ion channel drug potency assay with an artificial bilayer chip. Lab Chip 12, 2409-2413 (2012).
18. Baaken, G., Ankri, N., Schuler, A. K., Ruhe, J. & Behrends, J. C. Nanopore-Based Single-Molecule Mass Spectrometry on a Lipid MembraneMicroarray. Acs Nano 5, 8080-8088 (2011).
19. Zagnoni, M., Sandison, M. E. & Morgan, H. Microfluidic array platform for simultaneous lipid bilayer membrane formation. Biosensors & Bioelectronics 24, 1235-1240 (2009).
20. Andersson, M. et al. Voltage-induced gating of the mechanosensitive MscL ion channel reconstituted in a tethered lipid bilayer membrane. Biosensors & Bioelectronics 23, 919-923 (2008). (Pubitemid 350296952)
21. Suzuki, H. & Takeuchi, S. Microtechnologies for membrane protein studies. Anal. Bioanal. Chem. 391, 2695-2702 (2008).
22. Farre, C. & Fertig, N. HTS techniques for patch clamp-based ion channel screening-advances and economy. Expert Opinion on Drug Discovery 7, 515-524 (2012).
23. Funakoshi, K., Suzuki, H. & Takeuchi, S. Lipid bilayer formation by contacting monolayers in a microfluidic device for membrane protein analysis. Anal. Chem. 78, 8169-8174 (2006). (Pubitemid 44927591)
24. Bayley, H. et al. Droplet interface bilayers. Mol. Biosyst. 4, 1191-1208 (2008).
25. Thapliyal, T., Poulos, J. L.& Schmidt, J. J. Automated lipid bilayer and ion channel measurement platform. Biosensors & Bioelectronics 26, 2651-2654 (2011).
26. Kawano, R., Osaki, T., Sasaki, H. & Takeuchi, S. A Polymer-Based Nanopore-Integrated Microfluidic Device for Generating Stable Bilayer Lipid Membranes. Small 6, 2100-2104 (2010).
27. Kawano, R. et al. Rapid Detection of a Cocaine-Binding Aptamer Using Biological Nanopores on a Chip. J. Am. Chem. Soc. 133, 8474-8477 (2011).
28. Needham, D., McIntosh, T. J. & Lasic, D. D. Repulsive interactions and mechanical stability of polymer-grafted lipid-membranes. Biochim. Biophys. Acta 1108, 40-48 (1992).
29. Mayer, M., Kriebel, J. K., Tosteson, M. T. & Whitesides, G. M. Microfabricated teflon membranes for low-noise recordings of ion channels in planar lipid bilayers. Biophys. J. 85, 2684-2695 (2003). (Pubitemid 37210814)
30. Steinberg, D. S.Vibration analysis for electronic equipment, Edn. 3rd. (JohnWiley & Sons, New York; 2000).
31. Wonderlin,W. F., Finkel, A.& French, R. J. Optimizing planar lipid bilayer singlechannel recordings for high-resolution with rapid voltage steps. Biophys. J. 58, 289-297 (1990). (Pubitemid 20249658)
32. Le Pioufle, B., Suzuki, H., Tabata, K. V., Noji, H. & Takeuchi, S. Lipid bilayer microarray for parallel recording of transmembrane ion currents. Anal. Chem. 80, 328-332 (2008). (Pubitemid 351448439)
33. Hirano-Iwata, A. et al. Free-Standing Lipid Bilayers in Silicon Chips-Membrane Stabilization Based on Microfabricated Apertures with a Nanometer-Scale Smoothness. Langmuir 26, 1949-1952 (2010).
34. Labarca, P. & Latorre, R. Insertion of ion channels into planar lipid bilayers by vesicle fusion. Methods Enzymol. 207, 447-463 (1992).
35. Cordero-Morales, J. F. et al. Molecular determinants of gating at the potassiumchannel selectivity filter. Nat. Struct. Mol. Biol. 13, 311-318 (2006). (Pubitemid 43873501)
36. Hirano, M., Onishi, Y., Yanagida, T. & Ide, T. Role of the KcsA Channel Cytoplasmic Domain in pH-Dependent Gating. Biophys. J. 101, 2157-2162 (2011).
37. Butler, A., Tsunoda, S., McCobb, D. P., Wei, A. & Salkoff, L. Mslo, a complex mouse gene encoding maxi calcium-activated potassium channels. Science 261, 221-224 (1993). (Pubitemid 23265401)
38. Sun, X. P., Schlichter, L. C. & Stanley, E. F. Single-channel properties of BK-type calcium-activated potassium channels at a cholinergic presynaptic nerve terminal. Journal of Physiology-London 518, 639-651 (1999). (Pubitemid 29387236)
39. Yuan, C., O'Connell, R. J., Jacob, R. F., Mason, R. P. & Treistman, S. N. Requiation of the gating of BKCa channel by lipid bilayer thickness. J. Biol. Chem. 282, 7276-7286 (2007). (Pubitemid 47093666)
40. Giangiacomo, K. M., Garcia, M. L. & McManus, O. B. Mechanism of iberiotoxin block of the large-conductance calcium-activated potassium channel from bovine aortic smooth-muscle. Biochemistry 31, 6719-6727 (1992).
41. Harper, A. A., Catacuzzeno, L., Trequattrini, C., Petris, A. & Franciolini, F. Verapamil block of large-conductance Ca-activated K channels in rat aortic myocytes. J. Membr. Biol. 179, 103-111 (2001). (Pubitemid 32121603)
42. Arispe, N., Rojas, E., Pollard, H. B. Alzheimer disease amyloid beta protein forms calcium channels in bilayer membranes: blockade by tromethamine and aluminum. Proc. Natl. Acad. Sci. U.S.A. 90, 567-571 (1993). (Pubitemid 23028904)
43. Yamamoto, K. et al. Suppression of a Neocortical Potassium Channel Activity by Intracellular Amyloid-beta and Its Rescue with Homer1a. J. Neurosci. 31, 11100-11109 (2011).
44. LaFerla, F.M., Green, K. N. & Oddo, S. Intracellular amyloid-beta in Alzheimer's disease. Nat. Rev. Neurosci. 8, 499-509 (2007).
45. Good, T. A., Smith, D. O. & Murphy, R. M. beta-amyloid peptide blocks the fastinactivating K1 current in rat hippocampal neurons. Biophys. J. 70, 296-304 (1996).
46. Qi, J. S., Ye, L. & Qiao, J. T. Amyloid beta-protein fragment 31-35 suppresses delayed rectifying potassium channels in membrane patches excised from hippocampal neurons in rats. Synapse 51, 165-172 (2004). (Pubitemid 38036950)
47. Tsuji, Y. et al. Droplet-based lipid bilayer system integrated with microfluidic channels for solution exchange. Lab Chip 13, 1476-1481 (2013).
48. Yanagisawa, M., Iwamoto, M., Kato, A., Yoshikawa, K. & Oiki, S. Oriented Reconstitution of a Membrane Protein in a Giant Unilamellar Vesicle: Experimental Verification with the Potassium Channel KcsA. J. Am. Chem. Soc. 133, 11774-11779 (2011).