Microfabricated Teflon membranes for low-noise recordings of ion channels in planar lipid bilayers

Biophysical Journal

Volumn 85, Issue 4, 2003, Pages 2684-2695

  Mayer, Michael ^a,c   Kriebel, Jennah K ^a   Tosteson, Magdalena T ^b   Whitesides, George M ^a,c  

^a HARVARD UNIVERSITY   (United States)

^b HARVARD MEDICAL SCHOOL   (United States)

^c Box 53 ^*  (United States)

Author keywords

[No Author keywords available]

Indexed keywords

ALAMETHICIN; ION CHANNEL; POLITEF;

ARTICLE; CONDUCTANCE; ELECTRICITY; ELECTROPHYSIOLOGY; LIPID BILAYER; MEASUREMENT; MOLECULAR STABILITY; POROSITY; SIGNAL NOISE RATIO;

EID: 0141819130     PISSN: 00063495     EISSN: None     Source Type: Journal    
DOI: 10.1016/S0006-3495(03)74691-8     Document Type: Article

References (45)

1. Alvarez, O. 1986. How to set up a bilayer system. In Ion Channel Reconstitution. C. Miller, editor. Plenum Press, New York. 115-130.
2. Benndorf, K. 1995. Low-noise recording. In Single-Channel Recording. B. Sackmann and E. Neher, editors. Plenum Press, New York. 129-145.
3. Blinchikoff, H. J., and A. I. Zverev. 1976. Filtering in the Time and Frequency Domains. John Wiley & Sons, New York.
4. Boheim, G. 1974. Statistical analysis of alamethicin channels in black lipid membranes. J. Membr. Biol. 19:277-303.
5. Boheim, G., W. Hanke, and G. Jung. 1983. Alamethicin pore formation: voltage-dependent flip-flop of a-helix dipoles. Biophys. Struct. Mech. 9:181-191.
6. Borisenko, V., T. Lougheed, J. Hesse, N. Fertig, J. C. Behrends, A. Woolley, and G. J. Schuetz. 2003. Combined optical and electrical detection of gramicidin channel gating. Biophys. J. 84:612-622.
7. Colquhoun, D., and F. J. Sigworth. 1995. Fitting and statistical analysis of single-channel records. In Single-Channel Recording. B. Sakmann and E. Neher, editors. Plenum Press, New York. 483-587.
8. Denyer, J., J. Worley, B. Cox, G. Allenby, and M. Banks. 1998. HTS approaches to voltage-gated ion-channel drug discovery. Drug Discov. Today. 3:323-332.
9. Fertig, N., R. H. Blick, and J. C. Behrends. 2002. Whole cell patch clamp recording performed on a planar glass chip. Biophys. J. 82:3056-3062.
10. Fertig, N., C. Meyer, R. H. Blick, C. Trautmann, and J. C. Behrends. 2001. Microstructured glass chip for ion-channel electrophysiology. Phys. Rev. E. 64:040901-1-040901-4.
11. Fertig, N., A. Tilke, R. H. Blick, J. P. Kotthaus, J. C. Behrends, and G. ten Bruggencate.2000. Stable integration of isolated cell membrane patches in a nanomachined aperture. Appl. Phys. Lett. 77:1218-1220.
12. Fox, R. O., Jr., and F. M. Richards. 1982. A voltage-gated ion channel model inferred from the crystal structure of alamethicin at 1.5-A resolution. Nature. 300:325-330.
13. Hanke, W., and G. Boheim. 1980. The lowest conductance state of the alamethicin pore. Biochim. Biophys. Acta. 596:456-462.
14. Ide, T., and T. Yanagida. 1999. An artificial lipid bilayer formed on an agarose-coated glass for simultaneous electrical and optical measurement of single ion channels. Biochem. Biophys. Res. Commun. 265:595-599.
15. Lauger, P. 1979. Dielectric noise in membranes. Biochim. Biophys. Acta. 557:283-294.
16. Levis, R. A., and J. L. Rae. 1998. Low-noise patch-clamp techniques. Methods Enzymol. 293:218-266.
17. Lindquist, C. S. 1977. Active Network Design. Steward & Sons, Long Beach, CA.
18. Makohliso, S. A., L. Giovangrandi, D. Leonard, H. J. Mathieu, M. Ilegems, and P. Aebischer. 1998. Application of Teflon-AF thin films for biopatterning of neural cell adhesion. Biosens. Bioelectron. 13:1227-1235.
19. McGeoch, J. E., M. W. McGeoch, D. J. Carter, R. F. Shuman, and G. Guidotti. 2000. Biological-to-electronic interface with pores of ATP synthase subunit C in silicon nitride barrier. Med. Biol. Eng. Comput. 38:113-119.
20. Miller, C. 1986. Ion Channel Reconstitution. Plenum Press, New York.
21. Montal, M., and P. Mueller. 1972. Formation of bimolecular membranes from lipid monolayers and a study of their electrical properties. Proc. Natl. Acad. Sci. USA. 69:3561-3566.
22. Mueller, P., H. T. Tien, W. C. Wescott, and D. O. Rudin. 1962. Reconstitution of excitable cell membrane structure in vitro. Circulation. 26:1167-1171.
23. Pantoja, R., D. Sigg, R. Blunck, F. Bezanilla, and J. R. Heath. 2001. Bilayer reconstitution of voltage-dependent ion channels using a microfabricated silicon chip. Biophys. J. 81:2389-2394.
24. Penner, R. 1995. A practical guide to patch clamping. In Single-Channel Recording. B. Sakmann and E. Neher, editors. Plenum Press, New York. 3-30.
25. Peterman, M. C., J. M. Ziebarth, O. Braha, H. Bayley, H. A. Fishman, and D. M. Bloom. 2002. Ion channels and lipid bilayer membranes under high potentials using microfabricated apertures, Biomed. Microdevices. 4:231-236.
26. Pflueger, R. 1989. Physical constants of various polyamides. In Polymer Handbook. J. Brandrup and E. H. Immergut, editors. Wiley Interscience, New York. V/109-V/116.
27. Qin, D., Y. Xia, and G. M. Whitesides. 1996. Rapid prototyping of complex structures with feature sizes larger than 20 μm. Adv. Mater. 8:917-919.
28. Rae, J. L., and R. A. Levis. 1984. Patch clamp recordings from the epithelium of the lens obtained using glasses selected for low noise and improved sealing properties. Biophys. J. 45:144-146.
29. Rae, J. L., and R. A. Levis. 1992. A method for exceptionally low noise single channel recordings. Pflugers Arch. 420:618-620.
30. Resnick, P. R., and W. H. Buck. 1997. Teflon AF amorphous fluoropolymers. In Modern Fluoropolymers. J. Scheirs, editor. Wiley, Chichester, UK. 397-419.
31. Resnick, P. R., and W. H. Buck. 1999. Teflon AF a family of amorphous fluoropolymers with extraordinary properties. In Fluoropolymers 2: Properties. G. Hougham, editor. Plenum Press, New York. 25-33.
32. Reyes, J., and R. Latorre. 1979. Effect of the anesthetics benzyl alcohol and chloroform on bilayers made from monolayers. Biophys. J. 28:259-279.
33. Sakmann, B., and G. Boheim. 1979. Alamethicin-induced single channel conductance fluctuations in biological membranes. Nature. 282:336-339.
34. Sakmann, B., and E. Neher. 1995. Geometric parameters of pipettes and membrane patches. In Single-Channel Recording. B. Sakmann and E. Neher, editors. Plenum Press, New York. 637-650.
35. Schlue, W. R., and W. Hanke. 1993. Planar Lipid Bilayers. Academic Press, London.
36. Schmidt, C., M. Mayer, and H. Vogel. 2000. A chip-based biosensor for the functional analysis of single ion channels. Angew. Chem. Int. Ed. 39:3137-3140.
37. Sherman-Gold, R. 1993. The Axon Guide for Electrophysiology & Biophysics: Laboratory Techniques. Axon Instruments, Foster City, CA.
38. Tosteson, M. T., and M. Chow. 1997. Characterization of the ion channels formed by poliovirus in planar lipid membranes. J. Virol. 71:507-511.
39. Waldbillig, R. C., and G. Szabo. 1979. Planar bilayer membranes from pure lipids. Biochim. Biophys. Acta. 557:295-305.
40. White, S. H. 1986. The physical nature of planar bilayer membranes. In Ion Channel Reconstitution. C. Miller, editor. Plenum Press, New York. 3-35.
41. White, S. H., and T. E. Thompson. 1973. Capacitance, area, and thickness variations in thin lipid films. Biochim. Biophys. Acta. 323:7-22.
42. Whitesides, G. M., E. S. Ostuni, S. Takayama, X. Jiang, and D. E. Ingber. 2001. Soft lithography in biology and biochemistry. Annu. Rev. Biomed. Eng. 3:335-373.
43. Wonderlin, W. F., A. Finkel, and R. J. French. 1990. Optimizing planar lipid bilayer single-channel recordings for high resolution with rapid voltage steps. Biophys. J. 58:289-297.
44. Xia, Y., and G. M. Whitesides. 1998. Soft lithography. Angew. Chem. Int Ed. 37:550-575.
45. Xu, J., X. B. Wang, B. Ensign, M. Li, L. Wu, A. Guia, and J. Q. Xu. 2001. Ion-channel assay technologies: quo vadis? Drug Discov. Today. 6:1278-1287.