Optimization, purification and characterization of novel thermostable, haloalkaline, solvent stable protease from Bacillus halodurans CAS6 using marine shellfish wastes: A potential additive for detergent and antioxidant synthesis

Bioprocess and Biosystems Engineering

Volumn 36, Issue 7, 2013, Pages 873-883

  Annamalai, Neelamegam ^a   Rajeswari, Mayavan Veeramuthu ^b   Thavasi, Rengathavasi ^c   Vijayalakshmi, Shanmugam ^b   Balasubramanian, Thangavel ^b  

^a UNIVERSITY OF PUERTO RICO AT CAYEY   (Puerto Rico)

^b ANNAMALAI UNIVERSITY   (India)

^c POLYTECHNIC UNIVERSITY   (United States)

Author keywords

Antioxidant; Bacillus halodurans; Marine bacterium; Protease; Shellfish wastes; Thermostable

Indexed keywords

BACILLUS HALODURANS; MARINE BACTERIUM; PROTEASE; SHELLFISH WASTE; THERMOSTABLE;

ANTIOXIDANTS; BACTERIA; BACTERIOLOGY; ENZYMES; PURIFICATION; SOAPS (DETERGENTS); SUBMARINE GEOLOGY; SYNTHESIS (CHEMICAL);

SHELLFISH;

ALIPHATIC AMINE; BACTERIAL ENZYME; CYCLODEPSIPEPTIDE; CYCLOHEXANE; DETERGENT; NATURAL PRODUCT; ORGANIC SOLVENT; PROTEINASE;

ANIMAL PRODUCT; ANIMAL SHELL; ANTIOXIDANT ACTIVITY; ARTICLE; BACILLUS; BACILLUS HALODURANS; BACTERIAL STRAIN; BACTERIUM CULTURE; BACTERIUM ISOLATION; BIOREMEDIATION; CARBON SOURCE; CONTROLLED STUDY; ENZYME ACTIVITY; ENZYME ANALYSIS; ENZYME PURIFICATION; ENZYME SPECIFICITY; ENZYME STABILITY; ENZYME SYNTHESIS; HALOALKALOPHILE; INFRARED SPECTROSCOPY; MICROBIAL DEGRADATION; NONHUMAN; PRIORITY JOURNAL; PROCESS OPTIMIZATION; SHELLFISH; WATER CONTAMINATION;

ALKALIES; ANTIOXIDANTS; BACILLUS; BASE SEQUENCE; DETERGENTS; DNA PRIMERS; ENZYME STABILITY; FERMENTATION; HYDROGEN-ION CONCENTRATION; MARINE BIOLOGY; PEPTIDE HYDROLASES; SHELLFISH; SODIUM CHLORIDE; SPECTROSCOPY, FOURIER TRANSFORM INFRARED; SUBSTRATE SPECIFICITY; TEMPERATURE;

BACILLUS HALODURANS; BACTERIA (MICROORGANISMS); DECAPODA (CRUSTACEA);

EID: 84879555537     PISSN: 16157591     EISSN: 16157605     Source Type: Journal    
DOI: 10.1007/s00449-012-0820-3     Document Type: Article

References (32)

1. Banik RM, Prakash M (2004) Laundry detergent compatibility of the alkaline protease from Bacillus cereus. Microbiol Res 159:135-140
2. Bhaskar N, Sudeepa ES, Rashmi HN, Selvi AT (2007) Partial purification and characterization of protease of Bacillus proteolyticus CFR3001 isolated from fish processing waste and its antibacterial activities. Bioresour Technol 98:2758-2764
3. Jellouli K, Bougatef A, Manni L, Agrebi R, Siala R, Younes I, Nasri M (2009) Molecular and biochemical characterization of an extracellular serine-protease from Vibrio metschnikovii J1. J Ind Microbiol Biotechnol 36:939-948
4. Wang SL, Yang CH, Liang TW, Yen YH (2008) Optimization of conditions for protease production by Chryseobacterium taeanense TKU001. Bioresour Technol 99:3700-3707
5. Ma C, Ni X, Chi Z, Ma L, Gao L (2007) Purification and characterization of an alkaline protease from the marine yeast Aureobasidium pullulans for bioactive peptide production from different sources. Mar Biotechnol 9:343-351
6. Wang SL, Chao CH, Liang TW, Chen CC (2009) Purification and characterization of protease and chitinase from Bacillus cereus TKU006 and conversion of marine wastes by these enzymes. Mar Biotechnol 11:334-344
7. Viswanathan K, Omorebokhae R, Li G, Gross RA (2010) Protease-catalyzed oligomerization of hydrophobic amino acid ethyl esters in homogeneous reaction media using l-phenylalanine as a model system. Biomacromolecules 11:2152-2160
8. Chang WT, Chen YC, Jao CL (2007) Antifungal activity and enhancement of plant growth by Bacillus cereus grown on shellfish chitin wastes. Bioresour Technol 98:1224-1230
9. Sachindra NM, Bhaskar N (2008) In vitro antioxidant activity of liquor from fermented shrimp biowaste. Bioresour Technol 99:9013-9016
10. Nawani NN, Prakash D, Kapadnis BP (2010) Extraction, purification and characterization of an antioxidant from marine waste using protease and chitinase cocktail. World J Microbiol Biotechnol 26(8):1509-1517
11. Annamalai N, Rajeswari MV, Vijayalakshmi S, Balasubramanian T (2011) Purification and characterization of chitinase from Alcaligenes faecalis AU02 by utilizing marine wastes and its antioxidant activity. Ann Microbiol 61:801-807
12. Marmur J (1961) A procedure for the isolation of deoxyribonucleic acid from microorganisms. J Mol Biol 3:208-218
13. Marchesi J, Sato T, Andrew J, Martin T, Fry J, Hiom S (1998) Design and evaluation of useful bacterium specific PCR primers that amplify genes coding for bacterial 16S rRNA. Appl Environ Microbiol 64:795-799
14. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265-275
15. Todd EW (1949) Quantitative studies on the total plasmin and trypsin inhibitor of human blood serum. J Exp Med 39:295-308
16. Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227:680-685
17. Shimada K, Fujikawa K, Yahara K, Nakamura T (1992) Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem 40:945-948
18. Kadokura K, Rokutani A, Yamamoto M, Ikegami T, Sugita H, Itoi S, Hakamata W, Oku T, Nishio T (2007) Purification and characterization of Vibrio parahaemolyticus extracellular chitinase and chitin oligosaccharide deacetylase involved in the production of heterodisaccharide from chitin. Appl Microbiol Biotechnol 75:357-365
19. Wang SL, Shih IL, Liang TW, Wang CH (2002) Purification and characterization of two antifungal chitinases extracellularly produced by Bacillus amyloliquefaciens V656 in a shrimp and crab shell powder medium. J Agric Food Chem 50:2241-2248
20. Doddapaneni KK, Tatineni R, Vellanki RN, Rachcha S, Anabrolu N, Narakuti V, Mangamoori N (2008) Purification and characterization of a solvent and detergent-stable novel protease from Bacillus cereus. Microbiol Res 164:383-390
21. Sousa F, Jus S, Erbel A, Kokol V, Cavaco-Paulo A, Gubitz GM (2007) A novel metalloprotease from Bacillus cereus for protein fibre processing. Enzyme Microb Technol 40:1772-1781
22. Deng A, Wu J, Zhang Y, Zhang G, Wen T (2010) Purification and characterization of a surfactant-stable high-alkaline protease from Bacillus sp. B001. Bioresour Technol 101:7100-7106
23. Annamalai N, Aunkumar, Saravanakumar A, Vijayalakshmi S, Balasubramanian T (2011) Characterization of protease from Alcaligens faecalis and its antibacterial activity on fish pathogens. J Environ Biol 32:781-786
24. Haddar A, Agrebi R, Bougatef A, Hmidet N, Sellami-Kamoun A, Nasri M (2009) Two detergent stable alkaline serine-proteases from Bacillus mojavensis A21: purification, characterization and potential application as a laundry detergent additive. Bioresour Technol 100:3366-3373
25. Shivanand P, Jayaraman G (2009) Production of extracellular protease from halotolerant bacterium, Bacillus aquimaris strain VITP4 isolated from Kumta coast. Process Biochem 44:1088-1094
26. Karbalaei-Heidari HR, Ziaee A, Schaller J, Amoozegar MA (2007) Purification and characterization of an extracellular haloalkaline protease produced by the moderately halophilic bacterium, Salinivibrio sp. strain AF-2004. Enzyme Microb Technol 40:266-272
27. Arulmani M, Aparanjini K, Vasanthi K, Arumugam P, Arivuchelvi M, Kalaichelvan T (2006) Purification and partial characterization of serine protease from thermostable alkalophilic Bacillus laterosporus-AK1. World J Microbiol Biotechnol 23:475-481
28. Banerjee UC, Sani RK, Azmi W, Soni R (1999) Thermostable alkaline protease from Bacillus brevis and its characterization as a laundry detergent additive. Process Biochem 35:213-219
29. He H, Chen X, Sun C, Zhang Y, Gao P (2006) Preparation and functional evaluation of oligopeptide-enriched hydrolysate from shrimp (Acetes chinensis) treated with crude protease from Bacillus sp. SM98011. Bioresour Technol 97:385-390
30. Liang TW, Chen YJ, Yen YH, Wang SL (2007) The antitumor activity of the hydrolysates of chitinous materials hydrolyzed by crude enzyme from Bacillus amyloliquefaciens V656. Process Biochem 42:527-534
31. Binsan W, Benjakul S, Visessanguan W, Roytrakul S, Tanaka M, Kishimura H (2008) Antioxidative activity of mungoong, an extract paste, from the cephalothorax of white shrimp (Litopenaeus vannamei). Food Chem 106:185-193
32. Seymour TA, Li SJ, Morrissey MT (1996) Characterisation of a natural antioxidant from shrimp shell waste. J Agric Food Chem 44:682-685 Prediction System