Large scale production of the active human ASCT2 (SLC1A5) transporter in Pichia pastoris - Functional and kinetic asymmetry revealed in proteoliposomes

Biochimica et Biophysica Acta - Biomembranes

Volumn 1828, Issue 9, 2013, Pages 2238-2246

  Pingitore, Piero ^a,b   Pochini, Lorena ^a   Scalise, Mariafrancesca ^a   Galluccio, Michele ^a   Hedfalk, Kristina ^b   Indiveri, Cesare ^a  

^a UNIVERSITY OF CALABRIA   (Italy)

^b UNIVERSITY OF GOTHENBURG   (Sweden)

Author keywords

Liposomes; Over expression; Purification; Transport

Indexed keywords

ALANINE; AMINO ACID TRANSPORTER; ANTIPORTER; ASPARAGINE; CYTOSINE; DETERGENT; GLYCINE; METHIONINE; NEUTRAL AMINO ACID TRANSPORTER; NICKEL; PROTEOLIPOSOME; SERINE; SODIUM; THREONINE; UNCLASSIFIED DRUG; VALINE; 3 [(3 CHOLAMIDOPROPYL)DIMETHYLAMMONIO] 1 PROPANESULFONIC ACID; 3-((3-CHOLAMIDOPROPYL)DIMETHYLAMMONIUM)-1-PROPANESULFONATE; CYSTEINE; DIETHYL PYROCARBONATE; GLUTAMINE; LIPOSOME; MERCURY DERIVATIVE; MESYLIC ACID DERIVATIVE; METHANETHIOSULFONATE; PROTEOLIPID; PROTEOLIPOSOMES; SLC1A5 PROTEIN, HUMAN;

AMINO ACID TRANSPORT; ARTICLE; CHELATION; CONTROLLED STUDY; GEL FILTRATION CHROMATOGRAPHY; GENE OVEREXPRESSION; INHIBITION KINETICS; NONHUMAN; NUCLEOTIDE SEQUENCE; PH; PICHIA PASTORIS; PRIORITY JOURNAL; PROTEIN PURIFICATION; PROTEIN SYNTHESIS; PYROCOCCUS HORIKOSHII; STRUCTURAL HOMOLOGY; 2-(TRIMETHYLAMMONIUM)ETHYL METHANETHIOSULFONATE, BROMIDE; 2-AMINOBICYCLO-(2,2,1)-HEPTANE-2-CARBOXYLIC ACID; 2-AMINOETHYL METHANETHIOSULFONATE HYDROBROMIDE; A-(METHYLAMINO)ISOBUTYRIC ACID; BCH; BMGY; BUFFERED GLYCEROL-COMPLEX MEDIUM; C(12)E(8); CHEMICAL STRUCTURE; CHEMISTRY; DDM; DEPC; ENZYME SPECIFICITY; GENE EXPRESSION; GENETICS; HUMAN; KINETICS; LDAO; MEAIB; METABOLISM; MOLECULAR CLONING; MTSEA; MTSET; N-DODECYL-BETA-D-MALTOSIDE; N-DODECYL-N,N-DIMETHYLAMINE-N-OXIDE; N-ETHYLMALEIMIDE; N-PHENYLMALEIMIDE; NEM; OCTAETHYLENE GLYCOL MONODODECYL ETHER; OVER-EXPRESSION; P-HYDROXYMERCURIBENZOATE; P-OHMB; PEM; PICHIA; PLP; PURIFICATION; PYRIDOXAL-5-PHOSPHATE; SEQUENCE HOMOLOGY; TRANSPORT; TRANSPORT AT THE CELLULAR LEVEL; YEAST EXTRACT PEPTONE DEXTROSE SORBITOL; YPDS;

PICHIA PASTORIS; PYROCOCCUS HORIKOSHII;

2-(TRIMETHYLAMMONIUM)ETHYL METHANETHIOSULFONATE, BROMIDE; 2-AMINOBICYCLO-(2,2,1)-HEPTANE-2-CARBOXYLIC ACID; 2-AMINOETHYL METHANETHIOSULFONATE HYDROBROMIDE; 3-((3-CHOLAMIDOPROPYL)DIMETHYLAMMONIUM)-1-PROPANESULFONATE; A-(METHYLAMINO)ISOBUTYRIC ACID; BCH; BMGY; BUFFERED GLYCEROL-COMPLEX MEDIUM; C(12)E(8); CHAPS; DDM; DEPC; DIETHYL PYROCARBONATE; LDAO; LIPOSOMES; MEAIB; MTSEA; MTSET; N-DODECYL-BETA-D-MALTOSIDE; N-DODECYL-N,N-DIMETHYLAMINE-N-OXIDE; N-ETHYLMALEIMIDE; N-PHENYLMALEIMIDE; NEM; OCTAETHYLENE GLYCOL MONODODECYL ETHER; OVER-EXPRESSION; P-HYDROXYMERCURIBENZOATE; P-OHMB; PEM; PLP; PURIFICATION; PYRIDOXAL-5-PHOSPHATE; TRANSPORT; YEAST EXTRACT PEPTONE DEXTROSE SORBITOL; YPDS;

AMINO ACID TRANSPORT SYSTEM ASC; BIOLOGICAL TRANSPORT; CLONING, MOLECULAR; CYSTEINE; GENE EXPRESSION; GLUTAMINE; HUMANS; HYDROGEN-ION CONCENTRATION; KINETICS; MERCURY COMPOUNDS; MESYLATES; MODELS, MOLECULAR; PICHIA; PROTEOLIPIDS; SEQUENCE HOMOLOGY, AMINO ACID; SUBSTRATE SPECIFICITY;

EID: 84879473795     PISSN: 00052736     EISSN: 18792642     Source Type: Journal    
DOI: 10.1016/j.bbamem.2013.05.034     Document Type: Article

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