Directed evolution of phloroglucinol synthase PhlD with increased stability for phloroglucinol production

Applied Microbiology and Biotechnology

Volumn 97, Issue 13, 2013, Pages 5861-5867

  Rao, Guodong ^a   Lee, Jung Kul ^b   Zhao, Huimin ^a,c  

^a UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

^b Konkuk University   (South Korea)

^c UNIVERSITY OF ILLINOIS AT URBANA CHAMPAIGN   (United States)

Author keywords

Continuous product removal; Phloroglucinol; Protein engineering; Thermostability; Type III polyketide synthase

Indexed keywords

DIRECTED EVOLUTION; PHLOROGLUCINOL; PROTEIN ENGINEERING; RECOMBINANT ESCHERICHIA COLI; SITU PRODUCT REMOVAL; THERMAL INACTIVATION; THERMOSTABILITY; TYPE III POLYKETIDE SYNTHASE;

BIOCHEMICAL ENGINEERING; BIOCHEMISTRY; BIOSYNTHESIS; ENZYMES; ESCHERICHIA COLI; GENETIC ENGINEERING;

PRODUCTIVITY;

MALONYL COENZYME A; PH1D ENZYME; PHLOROGLUCINOL; SYNTHETASE; UNCLASSIFIED DRUG;

ALCOHOL; AROMATIC HYDROCARBON; BIOENGINEERING; COLIFORM BACTERIUM; PEPTIDE; POLLUTANT REMOVAL; PROTEIN; RECOMBINATION; STABILIZATION; TEMPERATURE EFFECT;

ARTICLE; DRUG MANUFACTURE; DRUG STABILITY; DRUG STRUCTURE; ENZYME STRUCTURE; ESCHERICHIA COLI; NONHUMAN;

BACTERIAL PROTEINS; DIRECTED MOLECULAR EVOLUTION; ENZYME STABILITY; ESCHERICHIA COLI; MALONYL COENZYME A; PHLOROGLUCINOL; PROTEIN ENGINEERING; PROTEIN STABILITY; RECOMBINANT PROTEINS; TEMPERATURE;

ESCHERICHIA COLI;

EID: 84879418259     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-013-4713-4     Document Type: Article

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