Effective inhibition of mRNA accumulation and protein expression of H5N1 avian influenza virus NS1 gene in vitro by small interfering RNAs

Folia Microbiologica

Volumn 58, Issue 4, 2013, Pages 335-342

  Jiao, Hanwei ^a   Du, Li ^a   Hao, Yongchang ^a   Cheng, Ying ^a   Luo, Jing ^b   Kuang, Wenhua ^c   Zhang, Donglin ^a   Lei, Ming ^a   Jia, Xiaoxiao ^a   Zhang, Xiaoru ^c   Qi, Chao ^c   He, Hongxuan ^b   Wang, Fengyang ^a  

^a HAINAN UNIVERSITY   (China)

^b INSTITUTE OF ZOOLOGY   (China)

^c CENTRAL CHINA NORMAL UNIVERSITY   (China)

Author keywords

[No Author keywords available]

Indexed keywords

ENHANCED GREEN FLUORESCENT PROTEIN; GREEN FLUORESCENT PROTEIN; HYBRID PROTEIN; INS1 PROTEIN, INFLUENZA VIRUS; SMALL INTERFERING RNA; VIRULENCE FACTOR; VIRUS PROTEIN;

ARTICLE; CELL LINE; DRUG ANTAGONISM; FLOW CYTOMETRY; GENE FUSION; GENETICS; GROWTH, DEVELOPMENT AND AGING; HUMAN; INFLUENZA VIRUS A H5N1; REAL TIME POLYMERASE CHAIN REACTION; REPORTER GENE; WESTERN BLOTTING;

ARTIFICIAL GENE FUSION; BLOTTING, WESTERN; CELL LINE; FLOW CYTOMETRY; GENES, REPORTER; GREEN FLUORESCENT PROTEINS; HUMANS; INFLUENZA A VIRUS, H5N1 SUBTYPE; REAL-TIME POLYMERASE CHAIN REACTION; RECOMBINANT FUSION PROTEINS; RNA, SMALL INTERFERING; VIRAL NONSTRUCTURAL PROTEINS; VIRULENCE FACTORS;

AVES; AVIAN INFLUENZA VIRUS;

EID: 84879175442     PISSN: 00155632     EISSN: None     Source Type: Journal    
DOI: 10.1007/s12223-012-0212-8     Document Type: Article

References (22)

1. Bender C, Hall H, Huang J, Klimov A, Cox N, Hay A, Gregory V, Cameron K, Lim W, Subbarao K (1999) Characterization of the surface proteins of influenza (H5N1) viruses isolated from humans in 1997-1998. Virology 254: 115-123.
2. Bryan RM, Steven FD (2009) The road to therapeutic RNA interference (RNAi) tackling the 800 pound siRNA delivery gorilla. Discover Med 8: 253-256.
3. Cullen BR (2002) RNA interference: antiviral defence and genetic tool. Nat Immunol 3: 597-599.
4. Ehrhardt C, Seyer R, Hrincius ER, Eierhoff T, Wolff T, Ludwig S (2010) Interplay between influenza A virus and the innate immune signaling. Microbes Infect 12: 81-87.
5. Ge Q, McManus MT, Nguyen T, Shen CH, Sharp PA, Eisen HN, Chen J (2003) RNA interference of influenza virus production by directly targeting mRNA for degradation and indirectly inhibiting all viral RNA transcription. Proc Natl Acad Sci U S A 100: 2718-2723.
6. Golebiewski L, Liu H, Javier RT, Rice AP (2011) The avian influenza virus NS1 ESEV PDZ binding motif associates with Dlg1 and Scibble to descript cellular tight junctions. J Virol 85: 10639-10648.
7. Hannon GJ (2002) RNA interference. Nature 418: 244-251.
8. Hatada E, Fukuda R (1992) Binding of influenza A virus NS1 protein to dsRNA in vitro. J Gen Virol 73(Pt12): 3325-3329.
9. Li Z, Jiang Y, Jiao P, Wang A, Zhao F, Tian G, Wang X, Yu K, Bu Z, Chen H (2006) The NS1 gene contributes to the virulence of H5N1 avian influenza viruses. J Virol 80: 11115-11123.
10. Liu H, Golebiewski L, Dow EC, Krug RM, Javier RT, Rice AP (2010) The ESEV PDZ-binding motif of the avian influenza A virus NS1 protein protects infected cells from apoptosis by directly targeting Scribble. J Virol 84: 11164-11174.
11. Liu T, Lei M, Jiao H, Du L, Cheng Y, Zhang D, Hao Y, Man C, Wang F (2011) RNA interference induces effective inhibition of mRNA accumulation and protein expression of SHEV ORF3 gene in vitro. Curr Microbiol 62: 1355-1362.
12. Long JX, Peng DX, Liu YL, Wu YT, Liu XF (2008) Virulence of H5N1 avian influenza virus enhanced by a 15-nucleotide deletion in the viral nonstructural gene. Virus Genes 36: 471-478.
13. Lu XZ, Yang GD, Zhang J, Fu HY, Jin L, Wei MY, Wang L, Lu ZF (2011) The sense strand pre-cleaved RNA duplex mediates an efficient RNA interference with less off-target and immune response effects. Appl Microbiol Biotechnol 90: 583-586.
14. Min JY, Krug RM (2006) The primary function of RNA binding by the influenza A virus NS1 protein in infected cells: inhibition the 2′-5′oligo (A) synthetase/RNase L pathway. Proc Natl Acad Sci U S A 103: 7100-7105.
15. Nemeroff ME, Qian XY, Krug RM (1995) The influenza virus NS1 protein forms multimers in vitro and in vivo. Virology 212: 422-428.
16. Panshin A, Golender N, Davidson I, Nagar S, Garsia M, Jackwood MW, Mundt E, Alturi A, Perk S (2010) Variability of NS1 proteins among H9N2 avian influenza viruses isolated in Israel during 2000-2009. Virus Genes 41: 396-405.
17. Spesock A, Malur M, Hossain MJ, Chen LM, Njaa BL, Davis CT, Lipatov AS, York IA, Krug RM, Donis RO (2011) The virulence of 1997 H5N1 influenza viruses in the mouse model is increased by correcting a defect in their NS1 proteins. J Virol 85: 7048-7069.
18. Sui HY, Zhao GY, Huang JD, Jin DY, Yuen KY, Zheng BJ (2009) Small interfering RNA targeting m2 gene induces effective and long term inhibition of influenza A virus replication. PLoS One 4: e5671.
19. Watson J (1979) Continuous proliferation of murine antigen-specific helper T lymphocytes in culture. J Exp Med 150: 1510-1519.
20. Xu X, Subbarao CNJ, Guo Y (1999) Genetic characterization of the pathogenic influenza A/Goose/Guangdong/1/96 (H5N1) virus: similarity of its hemagglutinin gene to those of H5N1 viruses from the 1997 outbreaks in Hong Kong. Virology 261: 15-19.
21. Zhang W, Wang CY, Yang ST, Qin C, Hu JL, Xia XZ (2009) Inhibition of highly pathogenic avian influenza virus H5N1 replication by the small interfering RNA targeting polymerase A gene. Biochem Biophys Res Commun 390: 421-426.
22. Zhou H, Jin M, Yu Z, Xu X, Peng Y, Wu H, Liu J, Liu H, Cao S, Chen H (2007) Effective small interfering RNAs targeting matrix and nucleocapside protein gene inhibit influenza A virus replication in cells and mice. Antiviral Res 76: 186-193.