Parallel visualization of multiple protein complexes in individual cells in tumor tissue

Molecular and Cellular Proteomics

Volumn 12, Issue 6, 2013, Pages 1563-1571

  Leuchowius, Karl Johan ^a,c   Clausson, Carl Magnus ^a   Grannas, Karin ^a   Erbilgin, Yücel ^b   Botling, Johan ^a   Zieba, Agata ^a   Landegren, Ulf ^a   Söderberg, Ola ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b ISTANBUL UNIVERSITY   (Turkey)

^c WALTER AND ELIZA HALL INSTITUTE OF MEDICAL RESEARCH   (Australia)

Author keywords

[No Author keywords available]

Indexed keywords

EPIDERMAL GROWTH FACTOR RECEPTOR; EPIDERMAL GROWTH FACTOR RECEPTOR 2; EPIDERMAL GROWTH FACTOR RECEPTOR 3; HETERODIMER; HOMODIMER; OLIGONUCLEOTIDE;

AMINO ACID SEQUENCE; ANIMAL CELL; ARTICLE; ASSAY; BREAST CANCER; CANCER TISSUE; CELL LEVEL; CELL POPULATION; CELLULAR DISTRIBUTION; HUMAN; HUMAN TISSUE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN INTERACTION; PROXIMITY LIGATION ASSAY; SIGNAL TRANSDUCTION; TISSUE SECTION;

ANIMALS; BIOPSY; BREAST NEOPLASMS; ENDOTHELIAL CELLS; FEMALE; GENE EXPRESSION REGULATION, NEOPLASTIC; HUMANS; IMMUNOCONJUGATES; MOLECULAR IMAGING; MOLECULAR PROBES; PROTEIN BINDING; PROTEIN INTERACTION MAPPING; PROTEIN MULTIMERIZATION; RECEPTOR, EPIDERMAL GROWTH FACTOR; RECEPTOR, ERBB-2; RECEPTOR, ERBB-3; SIGNAL TRANSDUCTION; SINGLE-CELL ANALYSIS; SWINE;

EID: 84878704360     PISSN: 15359476     EISSN: 15359484     Source Type: Journal    
DOI: 10.1074/mcp.O112.023374     Document Type: Article

References (28)

1. Kong, A., Leboucher, P., Leek, R., Calleja, V., Winter, S., Harris, A., Parker, P. J., and Larijani, B. (2006) Prognostic value of an activation state marker for epidermal growth factor receptor in tissue microarrays of head and neck cancer. Cancer Res. 66, 2834-2843
2. Mukherjee, A., Badal, Y., Nguyen, X. T., Miller, J., Chenna, A., Tahir, H., Newton, A., Parry, G., and Williams, S. (2011) Profiling the HER3/PI3K pathway in breast tumors using proximity-directed assays identifies correlations between protein complexes and phosphoproteins. PLoS One 6, e16443
3. Brockhoff, G., Heiss, P., Schlegel, J., Hofstaedter, F., and Knuechel, R. (2001) Epidermal growth factor receptor, c-erbB2 and c-erbB3 receptor interaction, and related cell cycle kinetics of SK-BR-3 and BT474 breast carcinoma cells. Cytometry 44, 338-348
4. Desmedt, C., Sperinde, J., Piette, F., Huang, W., Jin, X., Tan, Y., Durbecq, V., Larsimont, D., Giuliani, R., Chappey, C., Buyse, M., Winslow, J., Piccart, M., Sotiriou, C., Petropoulos, C., and Bates, M. (2009) Quantitation of HER2 expression or HER2:HER2 dimers and differential survival in a cohort of metastatic breast cancer patients carefully selected for trastuzumab treatment primarily by FISH. Diagn. Mol. Pathol. 18, 22-29
5. Ewing, R. M., Chu, P., Elisma, F., Li, H., Taylor, P., Climie, S., McBroom- Cerajewski, L., Robinson, M. D., O'Connor, L., Li, M., Taylor, R., Dharsee, M., Ho, Y., Heilbut, A., Moore, L., Zhang, S., Ornatsky, O., Bukhman, Y. V., Ethier, M., Sheng, Y., Vasilescu, J., Abu-Farha, M., Lambert, J. P., Duewel, H. S., Stewart, I. I., Kuehl, B., Hogue, K., Colwill, K., Gladwish,K., Muskat, B., Kinach, R., Adams, S. L., Moran, M. F., Morin, G. B., Topaloglou, T., and Figeys, D. (2007) Large-scale mapping of human protein-protein interactions by mass spectrometry. Mol. Syst. Biol. 3, 89
6. Söderberg, O., Gullberg, M., Jarvius, M., Ridderstråle, K., Leuchowius, K. J., Jarvius, J., Wester, K., Hydbring, P., Bahram, F., Larsson, L. G., and Landegren, U. (2006) Direct observation of individual endogenous protein complexes in situ by proximity ligation. Nat. Methods 3, 995-1000 (Pubitemid 44782698)
7. Jarvius, M., Paulsson, J., Weibrecht, I., Leuchowius, K.J., Andersson, A. C., Wählby, C., Gullberg, M., Botling, J., Sjöblom, T., Markova, B., Ostman, A., Landegren, U., and Söderberg, O. (2007) In situ detection of phosphorylated platelet-derived growth factor receptor using a generalized proximity ligation method. Mol. Cell. Proteomics 6, 1500-1509 (Pubitemid 47506163)
8. Leuchowius, K. J., Jarvius, M., Wickström, M., Rickardson, L., Landegren, U., Larsson, R., Söderberg, O., Fryknäs, M., and Jarvius, J. (2010) High content screening for inhibitors of protein interactions and post-translational modifications in primary cells by proximity ligation. Mol. Cell. Proteomics 9, 178-183
9. Liu, Y., Gu, J., Hagner-McWhirter, Å., Sathiyanarayanan, P., Gullberg, M., Söderberg, O., Johansson, J., Hammond, M., Ivansson, D., and Landegren, U. (2011) Western blotting via proximity ligation for high performance protein analysis. Mol. Cell. Proteomics 10, O111.011031
10. Yarden, Y., and Sliwkowski, M. X. (2001) Untangling the ErbB signalling network. Nat. Rev. Mol. Cell Biol. 2, 127-137 (Pubitemid 33674041)
11. Ménard, S., Tagliabue, E., Campiglio, M., and Pupa, S. M. (2000) Role of HER2 gene overexpression in breast carcinoma. J. Cell. Physiol. 182, 150-162 (Pubitemid 30022667)
12. Pinkas-Kramarski, R., Soussan, L., Waterman, H., Levkowitz, G., Alroy, I., Klapper, L., Lavi, S., Seger, R., Ratzkin, B. J., Sela, M., and Yarden, Y. (1996) Diversification of Neu differentiation factor and epidermal growth factor signaling by combinatorial receptor interactions. EMBO J. 15, 2452-2467 (Pubitemid 26151185)
13. DeFazio-Eli, L., Strommen, K., Dao-Pick, T., Parry, G., Goodman, L., and Winslow, J. (2011) Quantitative assays for the measurement of HER1- HER2 heterodimerization and phosphorylation in cell lines and breast tumors: applications for diagnostics and targeted drug mechanism of action. Breast Cancer Res. 13, R44
14. Leuchowius, K. J., Weibrecht, I., Landegren, U., Gedda, L., and Söderberg, O. (2009) Flow cytometric in situ proximity ligation analyses of protein interactions and post-translational modification of the epidermal growth factor receptor family. Cytometry A 75, 833-839
15. Spears, M., Taylor, K. J., Munro, A. F., Cunningham, C. A., Mallon, E. A., Twelves, C. J., Cameron, D. A., Thomas, J., and Bartlett, J. M. (2012) In situ detection of HER2:HER2 and HER2:HER3 protein-protein interactions demonstrates prognostic significance in early breast cancer. Breast Cancer Res. Treat. 132, 463-470
16. Liccardi, G., Hartley, J. A., and Hochhauser, D. (2011) EGFR nuclear translocation modulates DNA repair following cisplatin and ionizing radiation treatment. Cancer Res. 71, 1103-1114
17. Yamashita-Kashima, Y., Iijima, S., Yorozu, K., Furugaki, K., Kurasawa, M., Ohta, M., and Kaori, F.-O. (2011) Pertuzumab in combination with trastuzumab shows significantly enhanced antitumor activity in HER2-positive human gastric cancer xenograft models. Clin. Cancer Res. 17, 5060-5070
18. Pedersen, N. M., Breen, K., Rødland, M. S., Haslekås, C., Stang, E., and Madshus, I. H. (2009) Expression of epidermal growth factor receptor or ErbB3 facilitates geldanamycin-induced down-regulation of ErbB2. Mol. Cancer Res. 7, 275-284
19. Carpenter, A. E., Jones, T. R., Lamprecht, M. R., Clarke, C., Kang, I. H., Friman, O., Guertin, D. A., Chang, J. H., Lindquist, R. A., Moffat, J., Golland, P., and Sabatini, D. M. (2006) CellProfiler: image analysis software for identifying and quantifying cell phenotypes. Genome Biol. 7, R100
20. Schindelin, J., Arganda-Carreras, I., Frise, E., Kaynig, V., Longair, M., Pietzsch, T., Preibisch, S., Rueden, C., Saalfeld, S., Schmid, B., Tinevez, J.-Y., White, D. J., Hartenstein, V., Eliceiri, K., Tomancak, P., and Cardona, A. (2012) Fiji: an open-source platform for biological-image analysis. Nat. Methods 9, 676-682
21. Good, M. C., Zalatan, J. G., and Lim, W. A. (2011) Scaffold proteins: hubs for controlling the flow of cellular information. Science 332, 680-686
22. Conze, T., Carvalho, A. S., Landegren, U., Almeida, R., Reis, C. A., David, L., and Söderberg, O. (2010) MUC2 mucin is a major carrier of the cancer-associated sialyl-Tn antigen in intestinal metaplasia and gastric carcinomas. Glycobiology 20, 199-206
23. Clausson, C. M., Allalou, A., Weibrecht, I., Mahmoudi, S., Farnebo, M., Landegren, U., Wählby, C., and Söderberg, O. (2011) Increasing the dynamic range of in situ PLA. Nat. Methods 8, 892-893
24. Göransson, J., Wählby, C., Isaksson, M., Howell, W. M., Jarvius, J., and Nilsson, M. (2009) A single molecule array for digital targeted molecular analyses. Nucleic Acids Res. 37, e7
25. Weibrecht, I., Gavrilovic, M., Lindbom, L., Landegren, U., Wählby, C., and Söderberg, O. (2012) Visualising individual sequence-specific protein- DNA interactions in situ. N. Biotechnol. 29, 589-598
26. Hopkins, A. L. (2008) Network pharmacology: the next paradigm in drug discovery. Nat. Chem. Biol. 4, 682-690
27. Barabási, A. L., and Oltvai, Z. N. (2004) Network biology: understanding the cell's functional organization. Nat. Rev. Genet. 5, 101-113 (Pubitemid 38160277)
28. Csermely, P., Agoston, V., and Pongor, S. (2005) The efficiency of multitarget drugs: the network approach might help drug design. Trends Pharmacol. Sci. 26, 178-182 (Pubitemid 40432652)