Western blotting via proximity ligation for high performance protein analysis

Molecular and Cellular Proteomics

Volumn 10, Issue 11, 2011, Pages

  Liu, Yanling ^a,b   Gu, Jijuan ^a   Hagner McWhirter, Åsa ^b   Sathiyanarayanan, Poojahrau ^a   Gullberg, Mats ^c   Söderberg, Ola ^a   Johansson, Johan ^b   Hammond, Maria ^a   Ivansson, Daniel ^b   Landegren, Ulf ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b GE Healthcare Bio Sciences AB   (Sweden)

^c OLINK BIOSCIENCE   (Sweden)

Author keywords

[No Author keywords available]

Indexed keywords

DNA; PLATELET DERIVED GROWTH FACTOR BETA RECEPTOR; ANTIBODY; OLIGONUCLEOTIDE; TRANSFERRIN; TUBULIN;

ARTICLE; HUMAN; HUMAN CELL; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; SENSITIVITY AND SPECIFICITY; WESTERN BLOTTING; CELL CULTURE; CHEMISTRY; COMPARATIVE STUDY; LIMIT OF DETECTION; METABOLISM; METHODOLOGY; NUCLEIC ACID AMPLIFICATION; PHOSPHORYLATION; PROTEIN PROCESSING; SIGNAL NOISE RATIO;

ANTIBODIES; BLOTTING, WESTERN; CELLS, CULTURED; HUMANS; LIMIT OF DETECTION; NUCLEIC ACID AMPLIFICATION TECHNIQUES; OLIGONUCLEOTIDES; PHOSPHORYLATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; RECEPTOR, PLATELET-DERIVED GROWTH FACTOR BETA; SIGNAL-TO-NOISE RATIO; TRANSFERRIN; TUBULIN;

EID: 80555134726     PISSN: 15359476     EISSN: 15359484     Source Type: Journal    
DOI: 10.1074/mcp.O111.011031     Document Type: Article

References (16)

1. Burnette, W. N. (1981) "Western Blotting": Electrophoretic transfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to unmodified nitrocellulose and radiographic detection with antibody and radioiodinated protein A. Anal. Biochem. 112, 195-203
2. Vachereau, A. (1989) Anal. Biochem. 179, 206-208
3. Gallagher, S., Winston, S. E., Fuller, S. A., and Hurrell, J. G. (2004) Immunoblotting and immunodetection. Curr. Protoc. Neurosci, Unit 5.19
4. Kurien, B. T., and Scofield, R. H. (2006) Western Blotting Methods 38, 283-293
5. Rönn, O., Landström, M., Edman-Örlefors, A., and Edlund, S. (2005) Detection and quantitation of low-abundant proteins with ECL Plex fluorescent Western blotting. Discovery Matters, Issue 2
6. Wenz, C., and Rüfer, A. (2009) Microchip CGE linked to immunoprecipitation as an alternative to Western blotting. Electrophoresis 30, 4264-4269
7. Paimberg, E, Johnsen, J. I., Paulsson, J., Gleissman, H., Wickstrom, M., Edgren, M., Ostman, A., Kogner, P., and Lindskog, M. (2009) Metronomic scheduling of imatinib abrogates clonogenicity of neuroblastoma cells and enhances their susceptibility to selected chemotherapeutic drugs in vitro and in vivo. Int. J. Cancer 124, 1227-1234
8. Amersham Biosciences. Multiplex protein detection using the ECL Plex fluorescent western blotting system. Application note 28-4015-40 AA
9. Fredriksson, S., Gullberg, M., Jarvius, J., Olsson, C., Pietras, K., Gústafsdóttir, S. M., Östman, A., and Landegren, U. (2002) Protein detection using proximity-dependent DNA ligation assays. Nat. Biotechnol. 20, 473-477 (Pubitemid 34495933)
10. Fredriksson, S., Dixon, W., Ji, H., Koong, A. C., Mindrinos, M., and Davis, R. W. (2007) Multiplexed protein detection by proximity ligation for cancer biomarker validation. Nat. Methods 4, 327-329 (Pubitemid 46766977)
11. Schallmeiner, E., Oksanen, O., Ericsson, O., Spångberg, L., Eriksson, S., Stenman, U. H., Pettersson, K., and Landegren, U. (2007) Sensitive protein detection via triple-binder proximity ligation assays. Nat. Methods 4, 135-137 (Pubitemid 46454681)
12. Darmanis, S., Nong, R. Y., Hammond, M., Gu, J., Alderborn, A., Vänelid, J., Siegbahn, A., Gustafsdottir, S., Ericsson, O., Landegren, U., and Kamali-Moghaddam, M. (2010) Sensitive Plasma Protein Analysis by Microparticle-based Proximity Ligation Assays. Mol. Cell. Proteomics 9, 327-335
13. Söderberg, O., Gullberg, M., Jarvius, M., Ridderstråle, K., Leuchowius, K. J., Jarvius, J., Wester, K., Hydbring, P., Bahram, F., Larsson, L. G., and Landegren, U. (2006) Direct observation of individual endogenous protein complexes in situ by proximity ligation. Nat. Methods 3, 995-1000 (Pubitemid 44782698)
14. Jarvius, M., Paulsson, J., Weibrecht, I., Leuchowius, K. J., Andersson, A. C., Wählby, C., Gullberg, M., Botling, J., Sjöblom, T., Markova, B., Östman, A., Landegren, U., and Söderberg, O. (2007) In situ detection of phosphorylated PDGF receptor β using a generalized proximity ligation method. Mol. Cell. Proteomics 6, 1500-1509 (Pubitemid 47506163)
15. Leuchowius, K. J., Jarvius, M., Wickström, M., Rickardson, L., Landegren, U., Larsson, R., Söderberg, O., Fryknäs, M., and Jarvius, J. (2010) High content screening for inhibitors of protein interactions and post-translational modifications in primary cells by proximity ligation. Mol. Cell. Proteomics 9, 178-183
16. Blokzijl, A., Friedman, M., Pontén, F., and Landegren, U. (2010) Profiling protein expression and interactions: proximity ligation as a tool for personalized medicine. J. Intern. Med. 268, 232-245