In situ detection of phosphorylated platelet-derived growth factor receptor β using a generalized proximity ligation method

Molecular and Cellular Proteomics

Volumn 6, Issue 9, 2007, Pages 1500-1509

  Jarvius, Malin ^a   Paulsson, Janna ^b   Weibrecht, Irene ^e   Leuchowius, Karl Johan ^a   Anderson, Ann Catrin ^c   Wählby, Carolina ^a,d   Gullberg, Mats ^c   Botling, Johan ^a   Sjöblom, Tobias ^a   Markova, Boyka ^e   Östman, Arne ^b   Landegren, Ulf ^a   Söderberg, Ola ^a  

^a UPPSALA UNIVERSITY   (Sweden)

^b KAROLINSKA INSTITUTE   (Sweden)

^c OLINK BIOSCIENCE   (Sweden)

^d UPPSALA UNIVERSITY   (Sweden)

^e JOHANNES GUTENBERG UNIVERSITY   (Germany)

Author keywords

[No Author keywords available]

Indexed keywords

CIRCULAR DNA; IMMUNOGLOBULIN; INFLUENZA VIRUS HEMAGGLUTININ; PLATELET DERIVED GROWTH FACTOR ALPHA RECEPTOR; PLATELET DERIVED GROWTH FACTOR BB; PLATELET DERIVED GROWTH FACTOR BETA RECEPTOR; TELOMERASE REVERSE TRANSCRIPTASE; TYROSINE;

ANALYTIC METHOD; ANIMAL CELL; ANTIBODY SPECIFICITY; ARTERY ENDOTHELIUM; ARTICLE; CELL IMMORTALIZATION; CELL LINE; CELL STIMULATION; CELL STRAIN HEK293; CONTROLLED STUDY; DIAGNOSTIC VALUE; DNA STRAND; DRUG SCREENING; EMBRYO; ENDOTHELIUM CELL; FIBROBLAST; FROZEN SECTION; GENE AMPLIFICATION; GENE OVEREXPRESSION; GENETIC TRANSFECTION; HISTOPATHOLOGY; HUMAN; HUMAN CELL; IMMUNOBLOTTING; IMMUNOFLUORESCENCE; IMMUNOHISTOCHEMISTRY; MOLECULAR PROBE; MOLECULAR RECOGNITION; NONHUMAN; POLYACRYLAMIDE GEL ELECTROPHORESIS; PREPUCE; PRIORITY JOURNAL; PROTEIN ANALYSIS; PROTEIN PHOSPHORYLATION; PROTEIN PROCESSING; SCAR; SENSITIVITY AND SPECIFICITY; SIGNAL TRANSDUCTION; SPECIES DIFFERENCE; SWINE; TISSUE CULTURE; TISSUE SECTION; WOUND HEALING;

ACTINS; CELL LINE; ENDOTHELIAL CELLS; FIBROBLASTS; HUMANS; IMMUNOGLOBULINS; IMMUNOHISTOCHEMISTRY; KIDNEY; PHOSPHORYLATION; PROTEIN PROCESSING, POST-TRANSLATIONAL; PROTEOMICS; RECEPTOR, PLATELET-DERIVED GROWTH FACTOR BETA; SIGNAL TRANSDUCTION; TYROSINE; WOUND HEALING;

SUS;

EID: 34848917292     PISSN: 15359476     EISSN: None     Source Type: Journal    
DOI: 10.1074/mcp.M700166-MCP200     Document Type: Article

References (20)

1. Baselga, J. (2006) Targeting tyrosine kinases in cancer: the second wave Science 312, 1175-1178
2. Raines, E. W. (2004) PDGF and cardiovascular disease. Cytokine Growth Factor Rev. 15, 237-254
3. Seet, B. T., Dikic, I., Zhou, M. M., and Pawson, T. (2006) Reading protein modifications with interaction domains. Nat. Rev. Mol. Cell. Biol. 7, 473-483
4. Hart, C. E., Forstrom, J. W., Kelly, J. D., Seifert, R. A., Smith, R. A., Ross, R., Murray, M. J., and Bowen-Pope, D. F. (1988) Two classes of PDGF receptor recognize different isoforms of PDGF. Science 240, 1529-1531
5. Bergsten, E., Uutela, M., Li, X., Pietras, K., Östman, A., Heldin, C. H., Alitalo, K., and Eriksson, U. (2001) PDGF-D is a specific, protease-activated ligand for the PDGF β-receptor. Nat. Cell Biol. 3, 512-516
6. Heldin, C. H., Ostman, A., and Rönnstrand, L. (1998) Signal transduction via platelet-derived growth factor receptors. Biochim. Biophys. Acta 1378, F79-113
7. Haj, F. G., Verveer, P. J., Squire, A., Neel, B. G., and Bastiaens, P. I. (2002) Imaging sites of receptor dephosphorylation by PTP1B on the surface of the endoplasmic reticulum. Science 295, 1708-1711
8. Jones, S. M., and Kazlauskas, A. (2001) Growth-factor-dependent mitogenesis requires two distinct phases of signaling. Nat. Cell Biol. 3, 165-172
9. Söderberg, O., Gullberg, M., Jarvius, M., Ridderstråle, K., Leuchowius, K. J., Jarvius, J., Wester, K., Hydbring, P., Bahram, F., Larsson, L. G., and Landegren, U. (2006) Direct observation of individual enclogenous protein complexes in situ by proximity ligation. Nat. Methods 3, 995-1000
10. Fredriksson, S., Gullberg, M., Jarvius, J., Olsson, C., Pietras, K., Gústafsdóttir, S. M., Östman, A., and Landegren, U. (2002) Protein detection using proximity-dependent DNA ligation assays Nat. Biotechnol. 20, 473-477
11. Gullberg, M., Gústafsdóttir, S. M., Schallmeiner, E., Jarvius, J., Bjarnegård, M., Betsholtz, C., Landegren, U., and Fredriksson, S. (2004) Cytokine detection by antibody-based proximity ligation Proc. Natl. Acad. Sci. U. S. A. 101, 8420-8424
12. Banér, J., Nilsson, M., Mendel-Hartvig, M., and Landegren, U. (1998) Signal amplification of padlock probes by rolling circle replication. Nucleic. Acids. Res. 26, 5073-5078
13. Markova, B., Henrlich, P., Ronnstrand, L., and Böhmer, F. D. (2003) Identification of protein tyrosine phosphatases associating with the PDGF receptor. Biochemistry 42, 2691-2699
14. Tenev, T., Bohmer, S. A., Kaufmann, R., Frese, S., Bittorf, T., Beckers, T., and Böhmer, F. D. (2000) Perinuclear localization of the protein-tyrosine phosphatase SHP-1 and inhibition of epidermal growth factor-stimulated STAT-1/3 activation in A431 cells. Eur. J. Cell Biol. 79, 261-271
15. Lindblad, J., Wählby, C., Bengtsson, E., and Zaltsman, A. (2004) Image analysis for automatic segmentation of cytoplasms and classification of Racl activation. Cytometry A 57, 22-33
16. Allalou, A., van de Rijke, F. M., Jahangir Tarechi, R., Raap, A. K., and Wählby, C. (2007) Image based measurement of single cell mtDNA mutation load. SCIA 2007: 631-640
17. Claesson-Welsh, L. (1994) Platelet-derived growth factor receptor signals. J. Biol. Chem. 269, 32023-32026
18. Rajkumar, V. S., Shiwen, X., Boström, M., Leoni, P., Muddle, J., Ivarsson, M., Gerdin, B., Denton, C. P., Bou-Gharios, G., Black, C. M., and Abraham, D. J. (2006) Platelet-derived growth factor-β receptor activation is essential for fibroblast and pericyte recruitment during cutaneous wound healing. Am. J. Pathol. 169, 2254-2265
19. Östman, A. (2004) PDGF receptors - mediators of autocrine tumor growth and regulators of tumor vasculature and stroma. Cytokine Growth Factor Rev. 15, 275-286
20. Schallmeiner, E., Oksanen, E., Ericsson, O., Spångberg, L., Erksson, S., Stenman, U. H., Pettersson, K., and Landegren, U. (2007) Sensitive protein detection via triple-binder proximity ligation assays. Nat. Methods 4, 135-137