Object-based analysis of astroglial reaction and astrocyte subtype morphology after ischemic brain injury

Acta Neurobiologiae Experimentalis

Volumn 73, Issue 1, 2013, Pages 79-87

  Wagner, Daniel Christoph ^a,b   Scheibe, Johanna ^a   Glocke, Isabelle ^a   Weise, Gesa ^a,b   Deten, Alexander ^a,b   Boltze, Johannes ^a,b,c   Kranz, Alexander ^a,b  

^a FRAUNHOFER INSTITUTE FOR CELL THERAPY AND IMMUNOLOGY   (Germany)

^b Translational Center for Regenerative Medicine   (Germany)

^c MASSACHUSETTS GENERAL HOSPITAL   (United States)

Author keywords

Astrocytes; Brain ischemia; Confocal microscopy; Immunohistochemistry; Reactive astrogliosis

Indexed keywords

EID: 84878410011     PISSN: 00651400     EISSN: 16890035     Source Type: Journal    
DOI: None     Document Type: Article

References (34)

1. Baas D, Dalencon D, Fressinaud C, Vitkovic L, Sarlieve LL (1998) Oligodendrocyte-type-2 astrocyte (O-2A) progenitor cells express glutamine synthetase: Developmental and cell type-specific regulation. Mol Psychiatry 3: 356-361.
2. Barreto GE, Sun X, Xu L, Giffard RG (2011) Astrocyte proliferation following stroke in the mouse depends on distance from the infarct. PLoS One 6: e27881.
3. Benesova J, Hock M, Butenko O, Prajerova I, Anderova M, Chvatal A (2009) Quantification of astrocyte volume changes during ischemia in situ reveals two populations of astrocytes in the cortex of GFAP/EGFP mice. J Neurosci Res 87: 96-111.
4. Bolte S, Cordelieres FP (2006) A Guided tour into subcellular colocalization analysis in light microscopy. J Microsc 224: 213-232.
5. Cammer W (1990) Glutamine synthetase in the central nervous system is not confined to astrocytes. J Neuroimmunol 26: 173-178.
6. Comeau JW, Costantino S, Wiseman PW (2006) A guide to accurate fluorescence microscopy colocalization measurements. Biophys J 91: 4611-4622.
7. Costes SV, Daelemans D, Cho EH, Dobbin Z, Pavlakis G, Lockett S (2004) Automatic and quantitative measurement of protein-protein colocalization in live cells. Biophys J 86: 3993-4003.
8. Kim WR, Kim JY, Moon Y, Kim HJ, Kim H, Sun W (2012) Regional difference of reactive astrogliosis following traumatic brain injury revealed by HGFAP-GFP transgenic mice. Neurosci Lett 513: 155-159.
9. Kimelberg HK (2004) The problem of astrocyte identity. Neurochem Int 45: 191-202.
10. Liberto CM, Albrecht PJ, Herx LM, Yong VW, Levison SW (2004) Pro-regenerative properties of cytokine-activated astrocytes. J Neurochem 89: 1092-1100.
11. Manders EM, Stap J, Brakenhoff GJ, van DR, Aten JA (1992) Dynamics of three-dimensional replication patterns during the S-phase, analysed by double labelling of DNA and confocal microscopy. J Cell Sci 103: 857-862.
12. Menet V, Prieto M, Privat A, Ribotta M (2003) Axonal plasticity and functional recovery after spinal cord injury in mice deficient in both glial fibrillary acidic protein and vimentin genes. Proc Natl Acad Sci U S A 100: 8999-9004.
13. Okada S, Nakamura M, Katoh H, Miyao T, Shimazaki T, Ishii K, Yamane J, Yoshimura A, Iwamoto Y, Toyama Y, Okano H (2006) Conditional ablation of stat3 or socs3 discloses a dual role for reactive astrocytes after spinal cord injury. Nat Med 12: 829-834.
14. Oki K, Kaneko N, Kanki H, Imai T, Suzuki N, Sawamoto K, Okano H (2010) Musashi1 as a marker of reactive astrocytes after transient focal brain ischemia. Neurosci Res 66: 390-395.
15. Paxinos G, Watson C (2006) The Rat Brain in Stereotaxic Coordinates (6th ed). Academic Press, New York, NY.
16. Popa-Wagner A, Buga AM, Kokaia Z (2011) Perturbed cellular response to brain injury during aging. Ageing Res Rev 10: 71-79.
17. Riegelsberger UM, Deten A, Posel C, Zille M, Kranz A, Boltze J, Wagner DC (2011) Intravenous human umbilical cord blood transplantation for stroke: impact on infarct volume and caspase-3-dependent cell death in spontaneously hypertensive rats. Exp Neurol 227: 218-223.
18. Shimada IS, Borders A, Aronshtam A, Spees JL (2011) Proliferating reactive astrocytes are regulated by Notch-1 in the peri-infarct area after stroke. Stroke 42: 3231-3237.
19. Silver J, Miller JH (2004) Regeneration beyond the glial scar. Nat Rev Neurosci 5: 146-156.
20. Singan VR, Jones TR, Curran KM, Simpson JC (2011) Dual channel rank-based intensity weighting for quantitative co-localization of microscopy images. BMC Bioinformatics 12: 407.
21. Sofroniew MV (2009) Molecular dissection of reactive astrogliosis and glial scar formation. Trends Neurosci 32: 638-647.
22. Sofroniew MV, Vinters HV (2010) Astrocytes: Biology and pathology. Acta Neuropathol 119: 7-35.
23. Stahlberg A, Andersson D, Aurelius J, Faiz M, Pekna M, Kubista M, Pekny M (2011) Defining Cell populations with single-cell gene expression profiling: Correlations and identification of astrocyte subpopulations. Nucleic Acids Res 39: e24.
24. Sun D, Jakobs TC (2012) Structural remodeling of astrocytes in the injured CNS. Neuroscientist 18: 567-588.
25. Takahashi H, Matsumoto H, Kumon Y, Ohnishi T, Freeman C, Imai Y, Tanaka J (2007) Expression of heparanase in nestin-positive reactive astrocytes in ischemic lesions of rat brain after transient middle cerebral artery occlusion. Neurosci Lett 417: 250-254.
26. Tanaka K, Nogawa S, Suzuki S, Dembo T, Kosakai A (2003) Upregulation of oligodendrocyte progenitor cells associated with restoration of mature oligodendrocytes and myelination in peri-infarct area in the rat brain. Brain Res 989: 172-179.
27. Walz W (2000) Controversy surrounding the existence of discrete functional classes of astrocytes in adult gray matter. Glia 31: 95-103.
28. Wang K, Walz W (2003) Unusual topographical pattern of proximal astrogliosis around a cortical devascularizing lesion. J Neurosci Res 73: 497-506.
29. Wang K, Bekar LK, Furber K, Walz W (2004) Vimentinexpressing proximal reactive astrocytes correlate with migration rather than proliferation following focal brain injury. Brain Res 1024: 193-202.
30. White RE, McTigue DM, Jakeman LB (2010) Regional heterogeneity in astrocyte responses following contusive spinal cord injury in mice. J Comp Neurol 518: 1370-1390.
31. Wilhelmsson U, Li L, Pekna M, Berthold CH, Blom S, Eliasson C, Renner O, Bushong E, Ellisman M, Morgan TE, Pekny M (2004) Absence of glial fibrillary acidic protein and vimentin prevents hypertrophy of astrocytic processes and improves post-traumatic regeneration. J Neurosci 24: 5016-5021.
32. Wilhelmsson U, Bushong EA, Price DL, Smarr BL, Phung V, Terada M, Ellisman MH, Pekny M (2006) Redefining the concept of reactive astrocytes as cells that remain within their unique domains upon reaction to injury. Proc Natl Acad Sci U S A 103: 17513-17518.
33. Zamanian JL, Xu L, Foo LC, Nouri N, Zhou L, Giffard RG, Barres BA (2012) Genomic analysis of reactive astrogliosis. J Neurosci 32: 6391-6410.
34. Zou J, Wang YX, Mu HJ, Xiang J, Wu W, Zhang B, Xie P (2011) Down-regulation of glutamine synthetase enhances migration of rat astrocytes after in vitro injury. Neurochem Int 58: 404-413.