Clarification of recombinant proteins from high cell density mammalian cell culture systems using new improved depth filters

Biotechnology and Bioengineering

Volumn 110, Issue 7, 2013, Pages 1964-1972

  Singh, Nripen ^a   Pizzelli, Kara ^b   Romero, Jonathan K ^c   Chrostowski, James ^c   Evangelist, Greg ^c   Hamzik, James ^a   Soice, Neil ^a   Cheng, K S ^a  

^a Purification Product Development   (United States)

^b EMD Millipore   (United States)

^c BIOGEN   (United States)

Author keywords

Clarification; Depth filtration; Downstream purification; Filtration; Flocculation

Indexed keywords

DEPTH FILTRATION; DOWNSTREAM PURIFICATIONS; HIGH CELL DENSITY; HIGH PRODUCTIVITY; MAMMALIAN CELL CULTURE; MAMMALIAN SYSTEMS; PRETREATMENT CONDITIONS; PROCESS-RELATED IMPURITIES;

ACIDIFICATION; CELL CULTURE; CELLS; CENTRIFUGATION; CLARIFIERS; DEBRIS; FILTERS (FOR FLUIDS); FILTRATION; FLOCCULATION; IMPURITIES; MAMMALS; OPTIMIZATION; PARTICLE SIZE ANALYSIS; POLYMERS; PURIFICATION; RECOMBINANT PROTEINS;

CLARIFICATION;

RECOMBINANT PROTEIN;

ACIDIFICATION; ARTICLE; CELL CULTURE; CELL DENSITY; CENTRIFUGATION; FILTRATION; FLOCCULATION; PARTICLE SIZE; PRECIPITATION; PURIFICATION;

ANIMALS; BIOTECHNOLOGY; CELL AGGREGATION; CELL COUNT; CELL CULTURE TECHNIQUES; CHO CELLS; CRICETULUS; FILTRATION; RECOMBINANT PROTEINS;

MAMMALIA;

EID: 84878381052     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.24848     Document Type: Article

References (14)

1. max testing for practical microfiltration train scale-up in biopharmaceutical processing. Pharm Technol 19:64-76.
2. Kuriyel R, Zydney A. 2000. Sterile filtration and virus filtration. In: Desai M, editor. Methods in biotechnology, Vol. 9, downstream protein processing. Totowa, NJ: Humana Press. p 185-194.
3. Liu HF, Ma J, Winter C, Bayer R. 2010. Recovery and purification process development for monoclonal antibody production. mAbs 2(5):480-499.
4. Lutz H, Blanchard M, Abbott I, Parampalli A, Setiabudi G, Chiruvolu V, Noguchi M. 2009. Considerations for scaling up depth filtration of harvested cell culture fluid. BioPharm Int 22(3):58-66.
5. Ma J, Hoang H, Myint T, Peram T, Fahrner R, Chou JH. 2010. Using precipitation by polyamines as an alternative to chromatographic separation in antibody purification processes. J Chromatogr B Analyt Technol Biomed Life Sci 878:798-806.
6. Pailhes M, Lambalot C, Barloga R. 2004. Integration of centrifuges with depth filtration for optimized cell culture fluid clarification processes. BioProcess J 3(3):55-58.
7. Riske F, Schroeder J, Belliveau J, Xuezhen K, Kutzko J, Menon MK. 2007. The use of chitosan and flocculant in mammalian cell culture dramatically improves clarification throughput without adversely impacting monoclonal antibody recovery. J Biotechnol 128:813-823.
8. Roush D, Lu Y. 2008. Advances in primary recovery: Centrifugation and membrane technology. Biotechnol Prog 24:488-495.
9. Shukla AA, Kandula J. 2009. Harvest and recovery of monoclonal antibodies: Cell removal and clarification. In: Gottschalk U, editor. Process scale purification of antibodies. Hoboken, NJ: Wiley, Interscience. p. 53-78.
10. Thommes J, Etzel M. 2007. Alternatives to chromatographic separations. Biotechnol Prog 23:42-45.
11. Westoby M, Chrostowski J, Vilmorin P, Smelko JP, Romero JK. 2011. Effects of solution environment on mammalian cell fermentation broth properties: Enhanced impurity removal and clarification performance. Biotechnol Bioeng 108(1):50-58.
12. Westoby M, Roger J, Haverstock R, Pieracci J, Romero JK. 2010. Modeling industrial centrifugation of mammalian cell culture using a capillary based scale-down system. Biotechnol Bioeng 108(5):989-998.
13. Yavorsky DP, McGee S. 2002. Selection and sizing of clarification depth filters. Genetic Eng News 22(9):44-45.
14. Yigzaw Y, Piper R, Tran M, Shukla AA. 2006. Exploitation of the adsorptive properties of depth filters for host cell protein removal during monoclonal antibody purification. Biotechnol Prog 22(1):288-296.