Using precipitation by polyamines as an alternative to chromatographic separation in antibody purification processes

Journal of Chromatography B: Analytical Technologies in the Biomedical and Life Sciences

Volumn 878, Issue 9-10, 2010, Pages 798-806

  Ma, Junfen ^a   Hoang, Hai ^a   Myint, Thomas ^a   Peram, Thanmaya ^a   Fahrner, Robert ^a   Chou, Judy H ^a  

^a GENENTECH INC   (United States)

Author keywords

High throughput screening; Host cell proteins; Monoclonal antibody; Polyamine; Precipitation; Purification

Indexed keywords

HIGH THROUGHPUT SCREENING; HOST CELL PROTEIN; HOST CELL PROTEINS; POLYAMINE; POLYAMINES;

AFFINITY CHROMATOGRAPHY; AMINES; CHROMATOGRAPHIC ANALYSIS; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; IONIC STRENGTH; MONOCLONAL ANTIBODIES; PRECIPITATION (CHEMICAL); PROTEINS; PURIFICATION; SURFACE PLASMON RESONANCE; THROUGHPUT;

CELL CULTURE;

CELL PROTEIN; DNA; GENTAMICIN; INSULIN; MONOCLONAL ANTIBODY; POLY(4 VINYLPYRIDINE); POLY(DIMETHYLAMINE CO EPICHLOROHYDRINETHYLENEDIAMINE); POLY(N METHYLVINYLAMINE); POLYALLYLAMINE; POLYAMINE DERIVATIVE; POLYETHYLENEIMINE; POLYVINYLAMINE; SPERMINE; UNCLASSIFIED DRUG;

ARTICLE; CHEMICAL STRUCTURE; HIGH THROUGHPUT SCREENING; HOST CELL; ION EXCHANGE CHROMATOGRAPHY; IONIC STRENGTH; PH MEASUREMENT; PRECIPITATION; PRIORITY JOURNAL; PROTEIN PURIFICATION;

ANIMALS; ANTIBODIES, MONOCLONAL; CHO CELLS; CHROMATOGRAPHY, AFFINITY; CRICETINAE; CRICETULUS; FRACTIONAL PRECIPITATION; HUMANS; IMMUNOGLOBULIN G; OSMOLAR CONCENTRATION; POLYAMINES; RECOMBINANT FUSION PROTEINS;

EID: 77549086419     PISSN: 15700232     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jchromb.2010.01.044     Document Type: Article

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