Effects of solution environment on mammalian cell fermentation broth properties: Enhanced impurity removal and clarification performance

Biotechnology and Bioengineering

Volumn 108, Issue 1, 2011, Pages 50-58

  Westoby, Matthew ^a   Chrostowski, James ^a   De Vilmorin, Philippe ^a   Smelko, John Paul ^a   Romero, Jonathan K ^a  

^a BIOGEN   (United States)

Author keywords

Cell flocculation; Enhanced mammalian cell clarification; Impurity removal

Indexed keywords

CELLULAR DEBRIS; CLARIFICATION PERFORMANCE; COMPLEX MEDIA; CULTURE BROTHS; ELEVATED LEVEL; FEED STREAMS; FILTER CAPACITY; HIGH CELL DENSITY; IMPURITY REMOVAL; IN-CELL; IONIC COMPOSITION; MAMMALIAN CELL CULTURE; MAMMALIAN CELL FERMENTATION; MAMMALIAN CELLS; MAMMALIAN SYSTEMS; PROCESS-RELATED IMPURITIES; PRODUCT RECOVERY; RECOMBINANT PROTEIN; TANGENTIAL FLOW;

ANIMAL CELL CULTURE; CELLS; CLARIFICATION; CLARIFIERS; DEBRIS; FLOCCULATION; IMPURITIES; MAMMALS; MICROFILTRATION; OPTIMIZATION; PRECIPITATION (CHEMICAL); PROTEINS; PURIFICATION; REMOVAL;

CELL CULTURE;

ARTICLE; BIOREACTOR; BROTH DILUTION; CELL CULTURE; CELL DENSITY; CONTROLLED STUDY; FERMENTATION; FLOCCULATION; MAMMAL CELL; PARTICLE SIZE; PH; PRECIPITATION; PURIFICATION;

ANIMALS; BIOREACTORS; BUFFERS; CHO CELLS; CRICETINAE; CRICETULUS; CULTURE MEDIA; HYDROGEN-ION CONCENTRATION; IMMUNOGLOBULIN G; RECOMBINANT PROTEINS;

MAMMALIA;

EID: 78649413222     PISSN: 00063592     EISSN: 10970290     Source Type: Journal    
DOI: 10.1002/bit.22923     Document Type: Article

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