The use of chitosan as a flocculant in mammalian cell culture dramatically improves clarification throughput without adversely impacting monoclonal antibody recovery

Journal of Biotechnology

Volumn 128, Issue 4, 2007, Pages 813-823

  Riske, Frank ^a   Schroeder, James ^a   Belliveau, Julie ^a   Kang, Xuezhen ^a   Kutzko, Joseph ^a   Menon, Manoj K ^a  

^a GENZYME CORPORATION   (United States)

Author keywords

Cell culture harvest; Centrifugation; Chitosan; Clarification aid; Depth filtration; Monoclonal antibody

Indexed keywords

AGGLOMERATION; ANTIBODIES; CELL CULTURE; FILTRATION; TURBIDITY; WASTEWATER;

CELL CULTURE HARVEST; CLARIFICATION AIDS; DEPTH FILTRATION; MONOCLONAL ANTIBODY;

CHITIN;

CHITIN; CHITOSAN; HEAVY METAL; MONOCLONAL ANTIBODY; VOLATILE ORGANIC COMPOUND;

ANIMAL CELL; ARTICLE; BIOTECHNOLOGY; CELL CULTURE; CENTRIFUGATION; CONTROLLED STUDY; DEACETYLATION; FILTRATION; MOUSE; NONHUMAN; PARTICULATE MATTER; PRIORITY JOURNAL; REDUCTION; TURBIDITY; WASTE WATER;

ACETIC ACID; ANIMALS; ANTIBODIES, MONOCLONAL; BIOREACTORS; CELL CULTURE TECHNIQUES; CELL LINE; CHITOSAN; FLOCCULATION; IMMUNOGLOBULIN G;

ARTHROPODA; MAMMALIA;

EID: 33847349651     PISSN: 01681656     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jbiotec.2006.12.023     Document Type: Article

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