Single-molecule imaging can be achieved in live obligate anaerobic bacteria

Progress in Biomedical Optics and Imaging - Proceedings of SPIE

Volumn 8590, Issue , 2013, Pages

  Karunatilaka, Krishanthi S ^a   Coupland, Ben R ^a   Cameron, Elizabeth A ^b   Martens, Eric C ^b   Koropatkin, Nicole K ^b   Biteen, Julie S ^a  

^a UNIVERSITY OF MICHIGAN   (United States)

^b UNIVERSITY OF MICHIGAN MEDICAL SCHOOL   (United States)

Author keywords

anaerobic; bio imaging; FlAsH tag; HaloTag; single molecule fluorescence; starch catabolism; Super resolution imaging; symbiosis

Indexed keywords

ANAEROBIC; BIO-IMAGING; FLASH TAG; HALOTAG; SINGLE-MOLECULE FLUORESCENCES; SUPER RESOLUTION IMAGING; SYMBIOSIS;

FLUORESCENCE; IMAGE RECONSTRUCTION; OPTICAL RESOLVING POWER; PROTEINS; STARCH;

MOLECULES;

EID: 84878059910     PISSN: 16057422     EISSN: None     Source Type: Conference Proceeding    
DOI: 10.1117/12.2004204     Document Type: Conference Paper

References (16)

1. Moerner, W. E. and Fromm, D. P., "Methods of Single-Molecule Fluorescence Spectroscopy and Microscopy," Rev. Sci. Instrum. 74, 3597-3619 (2003).
2. Betzig, E., "Proposed method for molecular optical imaging," Opt. Lett. 20(3), 237-239 (1995).
3. Thompson, R. E., Larson, D. R. and Webb, W. W., "Precise nanometer localization analysis for individual fluorescent probes," Biophys. J. 82, 2775-2783 (2002).
4. Moerner, W. E., "New directions in single-molecule imaging and analysis," Proc. Natl. Acad. Sci. (U.S.A.) 104, 12596-12602 (2007).
5. Yang, H., "Progress in single-molecule spectroscopy in cells," Curr. Opin. Chem. Biol. 14(1), 3-9 (2010).
6. Cho, K. H. and Salyers, A. A., "Biochemical analysis of interactions between outer membrane proteins that contribute to starch utilization by Bacteroides thetaiotaomicron," J. Bacteriol. 183(24), 7224-7230 (2001).
7. Martens, E. C., Chiang, H. C. and Gordon, J. I., "Mucosal Glycan Foraging Enhances Fitness and Transmission of a Saccharolytic Human Gut Bacterial Symbiont," Cell Host & Microbe 4(5), 447-457 (2008).
8. Martens, E. C., Lowe, E. C., Chiang, H., Pudlo, N. A., Wu, M., McNulty, N. P., Abbott, D. W., Henrissat, B., Gilbert, H. J., Bolam, D. N. and Gordon, J. I., "Recognition and Degradation of Plant Cell Wall Polysaccharides by Two Human Gut Symbionts," Plos Biol. 9(12), e1001221 (2011).
9. Griffin, B. A., Adams, S. R. and Tsien, R. Y., "Specific covalent labeling of recombinant protein molecules inside live cells," Science 281, 269-272 (1998).
10. Heim, R., Prasher, D. C. and Tsien, R. Y., "Wavelength mutations and posttranslational autoxidation of green fluorescent protein," Proc. Natl. Acad. Sci. (U.S.A.) 91, 12501-12504 (1994).
11. Pan, N. and Imlay, J. A., "How does oxygen inhibit central metabolism in the obligate anaerobe Bacteroides thetaiotaomicron," Mol. Microbiol. 39(6), 1562-1571 (2001).
12. Martens, E. C., Roth, R., Heuser, J. E. and Gordon, J. I., "Coordinate Regulation of Glycan Degradation and Polysaccharide Capsule Biosynthesis by a Prominent Human Gut Symbiont," J. Biol. Chem. 284(27), 18445-18457 (2009).
13. Koropatkin, N. M., Martens, E. C., Gordon, J. I. and Smith, T. J., "Starch catabolism by a prominent human gut symbiont is directed by the recognition of amylose helices," Structure 16(7), 1105-1115 (2008).
14. Adams, S. R., Campbell, R. E., Gross, L. A., Martin, B. R., Walkup, G. K., Yao, Y., Llopis, J. and Tsien, R. Y., "New biarsenical ligands and tetracysteine motifs for protein labeling in vitro and in vivo: synthesis and biological applications," J. Am. Chem. Soc. 124, 6063-6076 (2002).
15. Koropatkin, N. M. and Smith, T. J., "SusG: A Unique Cell-Membrane-Associated alpha-Amylase from a Prominent Human Gut Symbiont Targets Complex Starch Molecules," Structure 18(2), 200-215 (2010).
16. Los, G. V., Encell, L. P., McDougall, M. G., Hartzell, D. D., Karassina, N., Zimprich, C., Wood, M. G., Learish, R., Ohana, R. F., Urh, M., Simpson, D., Mendez, J., Zimmerman, K., Otto, P., Vidugiris, G., Zhu, J., Darzins, A., Klaubert, D. H., Bulleit, R. F. and Wood, K. V., "HaloTag: A Novel Protein Labeling Technology for Cell Imaging and Protein Analysis," ACS Chem. Biol. 3(6), 373-382 (2008).