Simple resazurin-based microplate assay for measuring chlamydia infections

Antimicrobial Agents and Chemotherapy

Volumn 57, Issue 6, 2013, Pages 2838-2840

  Osaka, Ichie ^a   Hefty, P Scott ^a  

^a UNIVERSITY OF KANSAS   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

POLYMYXIN B; RESAZURIN; TETRACYCLINE;

ARTICLE; CELL VIABILITY; CHLAMYDIASIS; CONTROLLED STUDY; COST EFFECTIVENESS ANALYSIS; CYTOLYSIS; CYTOTOXICITY; DOSE RESPONSE; FLUORESCENCE MICROSCOPY; HOST CELL; NONHUMAN; PRIORITY JOURNAL;

ANIMALS; ANTI-BACTERIAL AGENTS; BIOLOGICAL ASSAY; CELL SURVIVAL; CHLAMYDIA INFECTIONS; CHLAMYDIA TRACHOMATIS; FIBROBLASTS; HUMANS; INDICATORS AND REAGENTS; L CELLS (CELL LINE); MICE; MICROBIAL SENSITIVITY TESTS; OXAZINES; POLYMYXIN B; TETRACYCLINE; XANTHENES;

EID: 84877851415     PISSN: 00664804     EISSN: 10986596     Source Type: Journal    
DOI: 10.1128/AAC.00056-13     Document Type: Article

References (19)

1. Gerbase AC, Rowley JT, Mertens TE. 1998. Global epidemiology of sexually transmitted diseases. Lancet 351(Suppl 3):2-4.
2. Wang S, Indrawati L, Wooters M, Caro-Aguilar I, Field J, Kaufhold R, Payne A, Caulfield MJ, Smith JG, Heinrichs JH. 2007. A novel automated method for enumeration of Chlamydia trachomatis inclusion forming units. J. Immunol. Methods 324:84-91.
3. Beeckman DS, Meesen G, Van Oostveldt P, Vanrompay D. 2009. Digital titration: Automated image acquisition and analysis of load and growth of Chlamydophila psittaci. Microsc. Res. Tech. 72:398-402.
4. Osaka I, Hills JM, Kieweg SL, Shinogle HE, Moore DS, Hefty PS. 2012. An automated image-based method for rapid analysis of Chlamydia infection as a tool for screening antichlamydial agents. Antimicrob. Agents Chemother. 56:4184-4188.
5. de Fries R, Mitsuhashi M. 1995. Quantification of mitogen induced human lymphocyte proliferation: Comparison of alamarBlue assay to 3Hthymidine incorporation assay. J. Clin. Lab. Anal. 9:89-95.
6. Ahmed SA, Gogal RM, Jr, Walsh JE. 1994. A new rapid and simple non-radioactive assay to monitor and determine the proliferation of lymphocytes: An alternative to [3H]thymidine incorporation assay. J. Immunol. Methods 170:211-224.
7. Al-Nasiry S, Geusens N, Hanssens M, Luyten C, Pijnenborg R. 2007. The use of Alamar Blue assay for quantitative analysis of viability, migration and invasion of choriocarcinoma cells. Hum. Reprod. 22:1304-1309.
8. Voytik-Harbin SL, Brightman AO, Waisner B, Lamar CH, Badylak SF. 1998. Application and evaluation of the alamarBlue assay for cell growth and survival of fibroblasts. In Vitro Cell. Dev. Biol. Anim. 34:239-246.
9. O'Brien J, Wilson I, Orton T, Pognan F. 2000. Investigation of the Alamar Blue (resazurin) fluorescent dye for the assessment of mammalian cell cytotoxicity. Eur. J. Biochem. 267:5421-5426.
10. Baker CN, Tenover FC. 1996. Evaluation of Alamar colorimetric broth microdilution susceptibility testing method for staphylococci and enterococci. J. Clin. Microbiol. 34:2654-2659.
11. Tiballi RN, He XG, Zarins LT, Revankar SG, Kauffman CA. 1995. Use of a colorimetric system for yeast susceptibility testing. J. Clin. Microbiol. 33:915-917.
12. Collins L, Franzblau SG. 1997. Microplate Alamar blue assay versus BACTEC 460 system for high-throughput screening of compounds against Mycobacterium tuberculosis and Mycobacterium avium. Antimicrob. Agents Chemother. 41:1004-1009.
13. Pettit RK, Weber CA, Pettit GR. 2009. Application of a high throughput Alamar blue biofilm susceptibility assay to Staphylococcus aureus biofilms. Ann. Clin. Microbiol. Antimicrob. 8:28.
14. Hybiske K, Stephens RS. 2007. Mechanisms of host cell exit by the intracellular bacterium Chlamydia. Proc. Natl. Acad. Sci. U. S. A. 104:11430-11435.
15. Zhang JH, Chung TDY, Oldenburg KR. 1999. A simple statistical parameter for use in evaluation and validation of high throughput screening assays. J. Biomol. Screen. 4:67-73.
16. Chopra I, Roberts M. 2001. Tetracycline antibiotics: Mode of action, applications, molecular biology, and epidemiology of bacterial resistance. Microbiol. Mol. Biol. Rev. 65:232-260.
17. Lampe MF, Ballweber LM, Isaacs CE, Patton DL, Stamm WE. 1998. Killing of Chlamydia trachomatis by novel antimicrobial lipids adapted from compounds in human breast milk. Antimicrob. Agents Chemother. 42:1239-1244.
18. Matsumoto A, Higashi N, Tamura A. 1973. Electron microscope observations on the effects of polymixin B sulfate on cell walls of Chlamydia psittaci. J. Bacteriol. 113:357-364.
19. Walsh M, Kappus EW, Quinn TC. 1987. In vitro evaluation of CP-62,993, erythromycin, clindamycin, and tetracycline against Chlamydia trachomatis. Antimicrob. Agents Chemother. 31:811-812.