A novel automated method for enumeration of Chlamydia trachomatis inclusion forming units

Journal of Immunological Methods

Volumn 324, Issue 1-2, 2007, Pages 84-91

  Wang, Su ^a   Indrawati, Lani ^a   Wooters, Melissa ^a   Caro Aguilar, Ivette ^a   Field, Jodie ^a   Kaufhold, Robin ^a   Payne, Angela ^a   Caulfield, Michael J ^a   Smith, Jeffrey G ^a   Heinrichs, Jon H ^a  

^a MERCK RESEARCH LABORATORIES   (United States)

Author keywords

Chlamydia trachomatis; Enumeration; ImmunoSpot; Inclusion forming unit

Indexed keywords

5 BROMO 4 CHLORO 3 INDOLYLPHOSPATE; ALKALINE PHOSPHATASE; FLUORESCENT DYE; LIPOPOLYSACCHARIDE; MONOCLONAL ANTIBODY; NITROBLUE TETRAZOLIUM; STREPTAVIDIN; UNCLASSIFIED DRUG;

ANIMAL CELL; ARTICLE; BACTERIAL COUNT; BIOTINYLATION; CELL INCLUSION; CELL LINE; CELL STRAIN L 929; CHLAMYDIA TRACHOMATIS; COLONY FORMING UNIT; COLORIMETRY; CONTROLLED STUDY; EPITHELIUM CELL; HELA CELL; HUMAN; HUMAN CELL; IMMUNOFLUORESCENCE TEST; INCUBATION TIME; INTERMETHOD COMPARISON; LABORATORY AUTOMATION; LABORATORY DIAGNOSIS; MICROSCOPY; NONHUMAN; PLATE COUNT; PRIORITY JOURNAL; TECHNIQUE;

ANIMALS; CELL LINE; CELL LINE, TUMOR; CHLAMYDIA TRACHOMATIS; COLONY COUNT, MICROBIAL; CRICETINAE; HELA CELLS; HUMANS; INCLUSION BODIES; MICE; MICE, INBRED BALB C;

ANIMALIA; CHLAMYDIA; CHLAMYDIA TRACHOMATIS;

EID: 34347247310     PISSN: 00221759     EISSN: None     Source Type: Journal    
DOI: 10.1016/j.jim.2007.05.004     Document Type: Article

References (11)

1. Atik B., Thanh T.T.K., Luong V.Q., Lagree S., and Dean D. Impact of annual targeted treatment on infectious trachoma and susceptibility to reinfection. JAMA 296 12 (2006) 1488-1497
2. Brunham R.C., and Rey-Ladino J. Immunology of Chlamydia infection: implications for a Chlamydia trachomatis vaccine. Nat. Rev. Immunol. 5 2 (2005) 149-161
3. Byrne G.I., Stephens R.S., Ada G., Caldwell H.D., Su H., Morrison R.P., et al. Workshop on in vitro neutralization of Chlamydia trachomatis: summary of proceedings. J. Infect. Dis. 168 2 (1993) 415-420
4. Hackstadt T. Cell Biology. In: Stephens R.S. (Ed). Chlamydia: Intracellular Biology, Pathogenesis, and Immunity (1999), ASM Press, Washington, D.C. 101-138
5. Kawa D.E., Schachter J., and Stephens R.S. Immune response to the Chlamydia trachomatis outer membrane protein PorB. Vaccine 22 31-32 (2004) 4282-4286
6. Liu X., Wang S., Sendi L., and Caulfield M.J. High-throughput imaging of bacterial colonies grown on filter plates with application to serum bactericidal assays. J. Immunol. Methods 292 1-2 (2004) 187-193
7. Miller W.C., Ford C.A., Morris M., Handcock M.S., Schmitz J.L., Hobbs M.M., et al. Prevalence of chlamydial and gonococcal infections among young adults in the United States. JAMA 291 18 (2004) 2229-2236
8. Morrison R.P., and Caldwell H.D. Immunity to murine chlamydial genital infection. Infect. Immun. 70 6 (2002) 2741-2751
9. Murdin A.D., Gellin B., Brunham R.C., Campbell L.A., Christiansen G., Deal C.D., et al. Collaborative multidisciplinary workshop report: progress toward a Chlamydia pneumoniae vaccine. J. Infect. Dis. 181 Suppl 3 (2000) S552-S557
10. Schachter J. Infection and disease epidemiology. In: Stephens R.S. (Ed). Chlamydia: Intracellular Biology, Pathogenesis, and Immunity (1999), ASM Press, Washington, D.C. 139-169
11. Stephens R.S. Genomic autobiographies of Chlamydiae. In: Stephens R.S. (Ed). Chlamydia; Intracellular Biology, Pathogenesis, and Immunity (1999), ASM Press, Washington, D.C. 9-27