Activation of RhoA in alcohol-induced intestinal barrier dysfunction

Inflammation

Volumn 36, Issue 3, 2013, Pages 750-758

  Tong, Jing ^a   Wang, Ying ^a   Chang, Bing ^a   Zhang, Dai ^a   Liu, Pengliang ^a   Wang, Bingyuan ^a  

^a CHINA MEDICAL UNIVERSITY   (China)

Author keywords

alcohol; iNOS; intestinal barrier dysfunction; RhoA

Indexed keywords

ALCOHOL; INDUCIBLE NITRIC OXIDE SYNTHASE; MESSENGER RNA; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN;

ANALYTICAL PARAMETERS; ANIMAL CELL; ANIMAL EXPERIMENT; ANIMAL MODEL; ANIMAL TISSUE; ARTICLE; CELL STRAIN CACO 2; CONTROLLED STUDY; ENTEROPATHY; ENZYME ACTIVATION; ENZYME ACTIVITY; ENZYME INHIBITION; ENZYME REGULATION; ENZYME SPECIFICITY; EPITHELIAL TRANSEPITHELIAL ELECTRICAL RESISTANCE VALUE; HUMAN; HUMAN CELL; IN VITRO STUDY; INTESTINAL BARRIER DYSFUNCTION; INTESTINE EPITHELIUM CELL; MALE; MOUSE; NONHUMAN; PATHOPHYSIOLOGY; PROTEIN EXPRESSION;

ALCOHOL DRINKING; ANIMALS; CACO-2 CELLS; CELL LINE, TUMOR; DISEASE MODELS, ANIMAL; ELECTRIC IMPEDANCE; ENZYME ACTIVATION; ETHANOL; HUMANS; INTESTINAL MUCOSA; INTESTINES; LYSINE; MALE; MICE; MICE, INBRED C57BL; NITRIC OXIDE SYNTHASE TYPE II; PERMEABILITY; RHO GTP-BINDING PROTEINS; RHOA GTP-BINDING PROTEIN; RNA INTERFERENCE; RNA, MESSENGER; RNA, SMALL INTERFERING;

MUS;

EID: 84877786629     PISSN: 03603997     EISSN: 15732576     Source Type: Journal    
DOI: 10.1007/s10753-013-9601-7     Document Type: Article

References (27)

1. Shaheen, N.J., R.A. Hansen, D.R. Morgan, L.M. Gangarosa, Y. Ringel, M.T. Thiny, M.W. Russo, and R.S. Sandler. 2006. The burden of gastrointestinal and liver diseases, 2006. American Journal of Gastroenterology 101(9): 2128-2138. doi: 10.1111/j.1572-0241.2006.00723.x.
2. Rehm, J., C. Mathers, S. Popova, M. Thavorncharoensap, Y. Teerawattananon, and J. Patra. 2009. Global burden of disease and injury and economic cost attributable to alcohol use and alcohol-use disorders. Lancet 373(9682): 2223-2233. doi: 10.1016/S0140-6736(09)60746-7.
3. Purohit, V., B. Gao, and B.J. Song. 2009. Molecular mechanisms of alcoholic fatty liver. Alcoholism, Clinical and Experimental Research 33(2): 191-205. doi: 10.1111/j.1530-0277.2008.00827.x.
4. Frazier, T.H., A.M. Stocker, N.A. Kershner, L.S. Marsano, and C.J. McClain. 2011. Treatment of alcoholic liver disease. Therapeutic Advances in Gastroenterology 4(1): 63-81. doi: 10.1177/1756283X10378925.
5. Keshavarzian, A., E.W. Holmes, M. Patel, F. Iber, J.Z. Fields, and S. Pethkar. 1999. Leaky gut in alcoholic cirrhosis: A possible mechanism for alcohol-induced liver damage. American Journal of Gastroenterology 94(1): 200-207. doi: 10.1111/j.1572-0241.1999.00797.x.
6. Parlesak, A., C. Schafer, T. Schutz, J.C. Bode, and C. Bode. 2000. Increased intestinal permeability to macromolecules and endotoxemia in patients with chronic alcohol abuse in different stages of alcohol-induced liver disease. Journal of Hepatology 32(5): 742-747.
7. Mackay, D.J., and A. Hall. 1998. Rho GTPases. Journal of Biological Chemistry 273(33): 20685-20688.
8. Bar-Sagi, D., and A. Hall. 2000. Ras and Rho GTPases: A family reunion. Cell 103(2): 227-238.
9. Etienne-Manneville, S., and A. Hall. 2002. Rho GTPases in cell biology. Nature 420(6916): 629-635. doi: 10.1038/nature01148.
10. Hopkins, A.M., S.V. Walsh, P. Verkade, P. Boquet, and A. Nusrat. 2003. Constitutive activation of Rho proteins by CNF-1 influences tight junction structure and epithelial barrier function. Journal of Cell Science 116(Pt 4): 725-742.
11. Bruewer, M., A.M. Hopkins, M.E. Hobert, A. Nusrat, and J.L. Madara. 2004. RhoA, Rac1, and Cdc42 exert distinct effects on epithelial barrier via selective structural and biochemical modulation of junctional proteins and F-actin. American Journal of Physiology. Cell Physiology 287(2): C327-C335. doi: 10.1152/ajpcell.00087.2004.
12. Shen, L., and J.R. Turner. 2006. Role of epithelial cells in initiation and propagation of intestinal inflammation. Eliminating the static: Tight junction dynamics exposed. American Journal of Physiology. Gastrointestinal and Liver Physiology 290(4): G577-G582. doi: 10.1152/ajpgi.00439.2005.
13. Schlegel, N., M. Meir, V. Spindler, C.T. Germer, and J. Waschke. 2011. Differential role of Rho GTPases in intestinal epithelial barrier regulation in vitro. Journal of Cellular Physiology 226(5): 1196-1203. doi: 10.1002/jcp.22446.
14. Iwata, T., F. Nozu, and M. Imawari. 2011. Ethanol impairs the assembly and disassembly of actin cytoskeleton and cell adhesion via the RhoA signaling pathway, catenin p120 and E-cadherin in CCK-stimulated pancreatic acini. Biochemical and Biophysical Research Communications 405(4): 558-563. doi: 10.1016/j.bbrc.2011.01.067.
15. Bailey, K.L., J.E. Robinson, J.H. Sisson, and T.A. Wyatt. 2011. Alcohol decreases RhoA activity through a nitric oxide (NO)/cyclic GMP(cGMP)/protein kinase G (PKG)-dependent pathway in the airway epithelium. Alcoholism, Clinical and Experimental Research 35(7): 1277-1281. doi: 10.1111/j.1530-0277.2011.01463. x.
16. Selva, J., and G. Egea. 2011. Ethanol increases p190RhoGAP activity, leading to actin cytoskeleton rearrangements. Journal of Neurochemistry 119(6): 1306-1316. doi: 10.1111/j.1471-4159.2011.07522.x.
17. Zhong, W., C.J. McClain, M. Cave, Y.J. Kang, and Z. Zhou. 2010. The role of zinc deficiency in alcohol-induced intestinal barrier dysfunction. American Journal of Physiology. Gastrointestinal and Liver Physiology 298(5): G625-G633. doi: 10.1152/ajpgi.00350.2009.
18. Zhong, W., Y. Zhao, C.J. McClain, Y.J. Kang, and Z. Zhou. 2010. Inactivation of hepatocyte nuclear factor-4{alpha} mediates alcohol-induced downregulation of intestinal tight junction proteins. American Journal of Physiology. Gastrointestinal and Liver Physiology 299(3): G643-G651. doi: 10.1152/ajpgi.00515.2009.
19. Livak, K.J., and T.D. Schmittgen. 2001. Analysis of relative gene expression data using real-time quantitative PCR and the 2(-Delta Delta C(T)) method. Methods 25(4): 402-408. doi: 10.1006/meth.2001.1262.
20. Romero, A.M., G. Esteban-Pretel, M.P. Marin, X. Ponsoda, R. Ballestin, J.J. Canales, and J. Renau-Piqueras. 2010. Chronic ethanol exposure alters the levels, assembly, and cellular organization of the actin cytoskeleton and microtubules in hippocampal neurons in primary culture. Toxicological Sciences 118(2): 602-612. doi: 10.1093/toxsci/kfq260.
21. Lindsley, T.A., S.N. Shah, and E.A. Ruggiero. 2011. Ethanol alters BDNF-induced Rho GTPase activation in axonal growth cones. Alcoholism, Clinical and Experimental Research 35(7): 1321-1330. doi: 10.1111/j.1530-0277.2011.01468. x.
22. Schaffert, C.S., S.L. Todero, C.A. Casey, G.M. Thiele, M.F. Sorrell, and D.J. Tuma. 2006. Chronic ethanol treatment impairs Rac and Cdc42 activation in rat hepatocytes. Alcoholism, Clinical and Experimental Research 30(7): 1208-1213. doi: 10.1111/j.1530-0277.2006.00135.x.
23. Forsyth, C.B., Y. Tang, M. Shaikh, L. Zhang, and A. Keshavarzian. 2011. Role of snail activation in alcohol-induced iNOS-mediated disruption of intestinal epithelial cell permeability. Alcoholism, Clinical and Experimental Research 35(9): 1635-1643. doi: 10.1111/j.1530-0277.2011.01510.x.
24. Tang, Y., C.B. Forsyth, A. Farhadi, J. Rangan, S. Jakate, M. Shaikh, A. Banan, J.Z. Fields, and A. Keshavarzian. 2009. Nitric oxide-mediated intestinal injury is required for alcohol-induced gut leakiness and liver damage. Alcoholism, Clinical and Experimental Research 33(7): 1220-1230. doi: 10.1111/j.1530-0277.2009.00946.x.
25. Soliman, H., G.P. Craig, P. Nagareddy, V.G. Yuen, G. Lin, U. Kumar, J.H. McNeill, and K.M. Macleod. 2008. Role of inducible nitric oxide synthase in induction of RhoA expression in hearts from diabetic rats. Cardiovascular Research 79(2): 322-330. doi: 10.1093/cvr/cvn095.
26. Li, D.B., Z.X. Fu, S.Q. Ruan, S.J. Hu, and X. Li. 2012. Acetylsalicylic acid regulates overexpressed small GTPase RhoA in vascular smooth muscle cells through prevention of new synthesis and enhancement of protein degradation. Bioscience Reports 32(2): 153-160. doi: 10.1042/BSR20110050.
27. Soliman, H., A. Gador, Y.H. Lu, G. Lin, G. Bankar, and K.M. Macleod. 2012. Diabetes-induced increased oxidative stress in cardiomyocytes is sustained by a positive feedback loop involving Rho kinase and PKCbeta2. American Journal of Physiology. Heart and Circulatory Physiology 303(8): H989-H1000. doi: 10.1152/ajpheart.00416.2012.