Ethanol impairs the assembly and disassembly of actin cytoskeleton and cell adhesion via the RhoA signaling pathway, catenin p120 and E-cadherin in CCK-stimulated pancreatic acini

Biochemical and Biophysical Research Communications

Volumn 405, Issue 4, 2011, Pages 558-563

  Iwata, Tomoyuki ^a   Nozu, Fumihiko ^a   Imawari, Michio ^a  

^a SHOWA UNIVERSITY SCHOOL OF MEDICINE   (Japan)

Author keywords

Actin cytoskeleton; Adherens protein; Ethanol; Pancreas; RhoA

Indexed keywords

ACTIN; ALCOHOL; AMYLASE; CATENIN; CHOLECYSTOKININ; CYTOSKELETON PROTEIN; PROTEIN P120; RHOA GUANINE NUCLEOTIDE BINDING PROTEIN; UVOMORULIN;

ACINAR CELL; ACTIN FILAMENT; AMYLASE RELEASE; ANIMAL CELL; ANIMAL TISSUE; ARTICLE; CELL ADHESION; CELL MEMBRANE; CONTROLLED STUDY; CYTOSKELETON; GENE EXPRESSION; GENE TRANSLOCATION; MALE; NONHUMAN; PRIORITY JOURNAL; PROTEIN EXPRESSION; PROTEIN PROTEIN INTERACTION; RAT; SIGNAL TRANSDUCTION; STIMULUS RESPONSE;

ACTINS; ANIMALS; CATENINS; CELL ADHESION; CELLS, CULTURED; CYTOSKELETON; ETHANOL; MALE; PANCREAS; PANCREATIC ALPHA-AMYLASES; RATS; RATS, SPRAGUE-DAWLEY; RHOA GTP-BINDING PROTEIN; SINCALIDE;

RATTUS;

EID: 79951951300     PISSN: 0006291X     EISSN: 10902104     Source Type: Journal    
DOI: 10.1016/j.bbrc.2011.01.067     Document Type: Article

References (36)

1. Pandol S.J., Saluja A.K., Imrie C.W., Banks P.A. Acute pancreatitis: bench to the bedside. Gastroenterology 2007, 132:1127-1151.
2. Saluja A.K., Bhagat L. Pathophysiology of alcohol-induced pancreatic injury. Pancreas 2003, 27:327-331.
3. Ponnappa B.C., Hoek J.B., Waring A.J., Rubin E. Effect of ethanol on amylase secretion and cellular calcium homeostasis in pancreatic acini from normal and ethanol-fed rats. Biochem. Pharmacol. 1987, 36:69-79.
4. Gukovskaya A.S., Hosseini S., Satoh A., Cheng J.H., Nam K.J., Gukovsky I., Pandol S.J. Ethanol differentially regulates NF-kappaB activation in pancreatic acinar cells through calcium and protein kinase C pathways. Am. J. Physiol. Gastrointest. Liver Physiol. 2004, 286:G204-G213.
5. Rizo J., Sudhof T.C. Snares and Munc18 in synaptic vesicle fusion. Nat. Rev. Neurosci. 2002, 3:641-653.
6. Gaisano H.Y. A hypothesis: SNARE-ing the mechanisms of regulated exocytosis and pathologic membrane fusions in the pancreatic acinar cell. Pancreas 2000, 20:217-226.
7. Cosen-Binker L.I., Lam P.P., Binker M.G., Reeve J., Pandol S., Gaisano H.Y. Alcohol/cholecystokinin-evoked pancreatic acinar basolateral exocytosis is mediated by protein kinase C alpha phosphorylation of Munc18c. J. Biol. Chem. 2007, 282:13047-13058.
8. Williams J.A., Chen X., Sabbatini M.E. Small G proteins as key regulators of pancreatic digestive enzyme secretion. Am. J. Physiol. Endocrinol. Metab. 2009, 296:E405-E414.
9. Heasman S.J., Ridley A.J. Mammalian Rho GTPases: new insights into their functions from in vivo studies. Nat. Rev. Mol. Cell Biol. 2008, 9:690-701.
10. Jaffe A.B., Hall A. Rho GTPases: biochemistry and biology. Annu. Rev. Cell Dev. Biol. 2005, 21:247-269.
11. Nozu F., Tsunoda Y., Ibitayo A.I., Bitar K.N., Owyang C. Involvement of RhoA and its interaction with protein kinase C and Src in CCK-stimulated pancreatic acini. Am. J. Physiol. 1999, 276:G915-G923.
12. Kim M., Nozu F., Kusama K., Imawari M. Cholecystokinin stimulates the recruitment of the Src-RhoA-phosphoinositide 3-kinase pathway by Vav-2 downstream of G(alpha13) in pancreatic acini. Biochem. Biophys. Res. Commun. 2006, 339:271-276.
13. Bi Y., Page S.L., Williams J.A. Rho and Rac promote acinar morphological changes, actin reorganization, and amylase secretion. Am. J. Physiol. Gastrointest. Liver Physiol. 2005, 289:G561-G570.
14. Bi Y., Williams J.A. A role for Rho and Rac in secretagogue-induced amylase release by pancreatic acini. Am J Physiol Cell Physiol 2005, 289:C22-C32.
15. Schafer C., Ross S.E., Bragado M.J., Groblewski G.E., Ernst S.A., Williams J.A. A role for the p38 mitogen-activated protein kinase/Hsp 27 pathway in cholecystokinin-induced changes in the actin cytoskeleton in rat pancreatic acini. J. Biol. Chem. 1998, 273:24173-24180.
16. Gooding J.M., Yap K.L., Ikura M. The cadherin-catenin complex as a focal point of cell adhesion and signaling: new insights from three-dimensional structures. BioEssays 2004, 26:497-511.
17. Reynolds A.B. p120-caten. Biochim. Biophys. Acta 2007, 1773:2-7.
18. Suzuki N., Hajicek N., Kozasa T. Regulation and physiological functions of G12/13-mediated signaling pathways. Neurosignals 2009, 17:55-70.
19. Leser J., Beil M.F., Musa O.A., Adler G., Lutz M.P. Regulation of adherens junction protein p120(ctn) by 10 nM CCK precedes actin breakdown in rat pancreatic acini. Am. J. Physiol. Gastrointest. Liver Physiol. 2000, 278:G486-G491.
20. Lerch M.M., Lutz M.P., Weidenbach H., Muller-Pillasch F., Gress T.M., Leser J., Adler G. Dissociation and reassembly of adherens junctions during experimental acute pancreatitis. Gastroenterology 1997, 113:1355-1366.
21. Siegmund E., Luthen F., Kunert J., Weber H. Ethanol modifies the actin cytoskeleton in rat pancreatic acinar cells--comparison with effects of CCK. Pancreatology 2004, 4:12-21.
22. Williams J.A., Korc M., Dormer R.L. Action of secretagogues on a new preparation of functionally intact, isolated pancreatic acini. Am. J. Physiol. 1978, 235:517-524.
23. Ren X.D., Kiosses W.B., Schwartz M.A. Regulation of the small GTP-binding protein Rho by cell adhesion and the cytoskeleton. EMBO J. 1999, 18:578-585.
24. Martinez S.E., Lazaro-Dieguez F., Selva J., Calvo F., Piqueras J.R., Crespo P., Claro E., Egea G. Lysophosphatidic acid rescues RhoA activation and phosphoinositides levels in astrocytes exposed to ethanol. J. Neurochem. 2007, 102:1044-1052.
25. Ho C., Shanmugasundararaj S., Miller K.W., Malinowski S.A., Cook A.C., Slater S.J. Interaction of anesthetics with the Rho GTPase regulator Rho GDP dissociation inhibitor. Biochemistry 2008, 47:9540-9552.
26. Schmidt A., Hall M.N. Signaling to the actin cytoskeleton. Annu. Rev. Cell Dev. Biol. 1998, 14:305-338.
27. Saluja A.K., Lerch M.M., Phillips P.A., Dudeja V. Why does pancreatic overstimulation cause pancreatitis?. Annu. Rev. Physiol. 2007, 69:249-269.
28. Allansson L., Khatibi S., Olsson T., Hansson E. Acute ethanol exposure induces [Ca2+]i transients, cell swelling and transformation of actin cytoskeleton in astroglial primary cultures. J. Neurochem. 2001, 76:472-479.
29. Slater S.J., Stagliano B.A., Seiz J.L., Curry J.P., Milano S.K., Gergich K.J., Stubbs C.D. Effects of ethanol on protein kinase C activity induced by filamentous actin. Biochim. Biophys. Acta 2001, 1544:207-216.
30. Banan A., Keshavarzian A., Zhang L., Shaikh M., Forsyth C.B., Tang Y., Fields J.Z. NF-kappaB activation as a key mechanism in ethanol-induced disruption of the F-actin cytoskeleton and monolayer barrier integrity in intestinal epithelium. Alcohol 2007, 41:447-460.
31. Joshi S., Guleria R.S., Pan J., Bayless K.J., Davis G.E., Dipette D., Singh U.S. Ethanol impairs Rho GTPase signaling and differentiation of cerebellar granule neurons in a rodent model of fetal alcohol syndrome. Cell. Mol. Life Sci. 2006, 63:2859-2870.
32. Perez-Moreno M., Jamora C., Fuchs E. Sticky business: orchestrating cellular signals at adherens junctions. Cell 2003, 112:535-548.
33. Anastasiadis P.Z. p120-ctn: A nexus for contextual signaling via Rho GTPases. Biochim. Biophys. Acta 2007, 1773:34-46.
34. Noren N.K., Liu B.P., Burridge K., Kreft B. p120 catenin regulates the actin cytoskeleton via Rho family GTPases. J. Cell Biol. 2000, 150:567-580.
35. Krakstad B.F., Ardawatia V.V., Aragay A.M. A role for Galpha12/Galpha13 in p120ctn regulation. Proc. Natl. Acad. Sci. USA 2004, 101:10314-10319.
36. Sabbatini M.E., Bi Y., Ji B., Ernst S.A., Williams J.A. CCK activates RhoA and Rac1 differentially through Galpha13 and Galphaq in mouse pancreatic acini. Am. J. Physiol. Cell Physiol. 2010, 298:C592-C601.