A Universal Vector for High-Efficiency Multi-Fragment Recombineering of BACs and Knock-In Constructs

PLoS ONE

Volumn 8, Issue 4, 2013, Pages

  Dolt, Karamjit Singh ^a,b   Lawrence, Melanie L ^a   Miller Hodges, Eve ^a   Slight, Joan ^a   Thornburn, Anna ^a   Devenney, Paul S ^a   Hohenstein, Peter ^a,b  

^a UNIVERSITY OF EDINBURGH   (United Kingdom)

^b UNIVERSITY OF EDINBURGH   (United Kingdom)

Author keywords

[No Author keywords available]

Indexed keywords

ANIMAL EXPERIMENT; ANIMAL TISSUE; ARTICLE; BACTERIAL ARTIFICIAL CHROMOSOME; CONTROLLED STUDY; DNA MODIFICATION; EMBRYO; EXPRESSION VECTOR; GENE CASSETTE; GENE CONSTRUCT; GENE TARGETING; GENETIC ENGINEERING; GENETIC RECOMBINATION; GENOMIC FRAGMENT; MOUSE; NONHUMAN; TRANSGENIC MOUSE; TRANSGENICS; UNIVERSAL VECTOR; ANIMAL; FEMALE; GENE VECTOR; GENETICS; PROCEDURES;

MUS MUSCULUS;

ANIMALS; CHROMOSOMES, ARTIFICIAL, BACTERIAL; FEMALE; GENE KNOCK-IN TECHNIQUES; GENETIC ENGINEERING; GENETIC VECTORS; MICE; RECOMBINATION, GENETIC;

EID: 84876907484     PISSN: None     EISSN: 19326203     Source Type: Journal    
DOI: 10.1371/journal.pone.0062054     Document Type: Article

References (28)

1. Kobayashi A, Valerius MT, Mugford JW, Carroll TJ, Self M, et al. (2008) Six2 defines and regulates a multipotent self-renewing nephron progenitor population throughout mammalian kidney development. Cell Stem Cell 3: 169-181.
2. Szymczak AL, Workman CJ, Wang Y, Vignali KM, Dilioglou S, et al. (2004) Correction of multi-gene deficiency in vivo using a single 'self-cleaving' 2A peptide-based retroviral vector. Nat Biotechnol 22: 589-594.
3. Anastassiadis K, Fu J, Patsch C, Hu S, Weidlich S, et al. (2009) Dre recombinase, like Cre, is a highly efficient site-specific recombinase in E. coli, mammalian cells and mice. Dis Model Mech 2: 508-515.
4. Nern A, Pfeiffer BD, Svoboda K, Rubin GM, (2011) Multiple new site-specific recombinases for use in manipulating animal genomes. Proc Natl Acad Sci U S A 108: 14198-14203.
5. Giraldo P, Montoliu L, (2001) Size matters: use of YACs, BACs and PACs in transgenic animals. Transgenic Res 10: 83-103.
6. Copeland NG, Jenkins NA, Court DL, (2001) Recombineering: a powerful new tool for mouse functional genomics. Nat Rev Genet 2: 769-779.
7. Poser I, Sarov M, Hutchins JR, Heriche JK, Toyoda Y, et al. (2008) BAC TransgeneOmics: a high-throughput method for exploration of protein function in mammals. Nat Methods 5: 409-415.
8. Skarnes WC, Rosen B, West AP, Koutsourakis M, Bushell W, et al. (2011) A conditional knockout resource for the genome-wide study of mouse gene function. Nature 474: 337-342.
9. Sasaki Y, Sone T, Yoshida S, Yahata K, Hotta J, et al. (2004) Evidence for high specificity and efficiency of multiple recombination signals in mixed DNA cloning by the Multisite Gateway system. J Biotechnol 107: 233-243.
10. Liu P, Jenkins NA, Copeland NG, (2003) A highly efficient recombineering-based method for generating conditional knockout mutations. Genome Res 13: 476-484.
11. Chan W, Costantino N, Li R, Lee SC, Su Q, et al. (2007) A recombineering based approach for high-throughput conditional knockout targeting vector construction. Nucleic Acids Res 35: e64.
12. Sharan SK, Thomason LC, Kuznetsov SG, Court DL, (2009) Recombineering: a homologous recombination-based method of genetic engineering. Nat Protoc 4: 206-223.
13. Hohenstein P, Slight J, Ozdemir DD, Burn SF, Berry R, et al. (2008) High-efficiency Rosa26 knock-in vector construction for Cre-regulated overexpression and RNAi. Pathogenetics 1: 3.
14. George SH, Gertsenstein M, Vintersten K, Korets-Smith E, Murphy J, et al. (2007) Developmental and adult phenotyping directly from mutant embryonic stem cells. Proc Natl Acad Sci U S A 104: 4455-4460.
15. Martinez-Estrada OM, Lettice LA, Essafi A, Guadix JA, Slight J, et al. (2010) Wt1 is required for cardiovascular progenitor cell formation through transcriptional control of Snail and E-cadherin. Nat Genet 42: 89-93.
16. Gertsenstein M, Nutter LM, Reid T, Pereira M, Stanford WL, et al. (2010) Efficient generation of germ line transmitting chimeras from C57BL/6N ES cells by aggregation with outbred host embryos. PLoS One 5: e11260.
17. Yusa K, Rad R, Takeda J, Bradley A, (2009) Generation of transgene-free induced pluripotent mouse stem cells by the piggyBac transposon. Nat Methods 6: 363-369.
18. Luche H, Weber O, Nageswara Rao T, Blum C, Fehling HJ, (2007) Faithful activation of an extra-bright red fluorescent protein in "knock-in" Cre-reporter mice ideally suited for lineage tracing studies. Eur J Immunol 37: 43-53.
19. Costantini F, Kopan R, (2010) Patterning a complex organ: branching morphogenesis and nephron segmentation in kidney development. Dev Cell 18: 698-712.
20. Self M, Lagutin OV, Bowling B, Hendrix J, Cai Y, et al. (2006) Six2 is required for suppression of nephrogenesis and progenitor renewal in the developing kidney. EMBO J 25: 5214-5228.
21. Karner CM, Das A, Ma Z, Self M, Chen C, et al. (2011) Canonical Wnt9b signaling balances progenitor cell expansion and differentiation during kidney development. Development 138: 1247-1257.
22. Park JS, Ma W, O'Brien LL, Chung E, Guo JJ, et al. (2012) Six2 and Wnt Regulate Self-Renewal and Commitment of Nephron Progenitors through Shared Gene Regulatory Networks. Dev Cell 23: 637-651.
23. Silva J, Nichols J, Theunissen TW, Guo G, van Oosten AL, et al. (2009) Nanog is the gateway to the pluripotent ground state. Cell 138: 722-737.
24. Chambers I, Silva J, Colby D, Nichols J, Nijmeijer B, et al. (2007) Nanog safeguards pluripotency and mediates germline development. Nature 450: 1230-1234.
25. Li MZ, Elledge SJ, (2007) Harnessing homologous recombination in vitro to generate recombinant DNA via SLIC. Nat Methods 4: 251-256.
26. Zhang Y, Werling U, Edelmann W, (2012) SLiCE: a novel bacterial cell extract-based DNA cloning method. Nucleic Acids Res 40: e55.
27. Hockemeyer D, Wang H, Kiani S, Lai CS, Gao Q, et al. (2011) Genetic engineering of human pluripotent cells using TALE nucleases. Nat Biotechnol 29: 731-734.
28. Urnov FD, Miller JC, Lee YL, Beausejour CM, Rock JM, et al. (2005) Highly efficient endogenous human gene correction using designed zinc-finger nucleases. Nature 435: 646-651.