Rational and efficient preparation of a chimeric protein containing a tandem dimer of thrombopoietin mimetic peptide fused to human growth hormone in Escherichia coli

Applied Microbiology and Biotechnology

Volumn 97, Issue 7, 2013, Pages 2885-2894

  Wang, Song ^a   Shen, Mingqiang ^a   Xu, Yang ^a   Chen, Fang ^a   Chen, Mo ^a   Chen, Shilei ^a   Wang, Aiping ^a   Zhang, Zhou ^a   Ran, Xinze ^a   Cheng, Tianmin ^a   Su, Yongping ^a   Wang, Junping ^a  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Escherichia coli; Fusion protein; Human growth hormone; Preparation; Thrombopoietin mimetic peptide

Indexed keywords

EQUIMOLAR CONCENTRATIONS; FUSION PROTEINS; HEMATOPOIETIC GROWTH FACTOR; HIGH LEVEL EXPRESSION; HUMAN GROWTH HORMONE; MALTOSE BINDING PROTEINS; PREPARATION; THROMBOPOIETIN;

AMINO ACIDS; BINDING SITES; BIOACTIVITY; ESCHERICHIA COLI; GENE ENCODING; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; HYDROPHOBIC CHROMATOGRAPHY; MASS SPECTROMETRY; PEPTIDES; RECOMBINANT PROTEINS; THERMOMECHANICAL PULPING PROCESS;

BIOMIMETICS;

CHIMERIC PROTEIN; DIMER; GROWTH HORMONE; HYBRID PROTEIN; THROMBOPOIETIN;

AMINO ACID; BIOACTIVITY; COLIFORM BACTERIUM; FILTRATION; GENETIC ENGINEERING; HORMONE; HYDROPHOBICITY; MASS SPECTROMETRY; PEPTIDE; PLASMID; PROTEIN; RESIN; SOLUBILIZATION;

ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; ESCHERICHIA COLI; GEL FILTRATION; HIGH PERFORMANCE LIQUID CHROMATOGRAPHY; MASS SPECTROMETRY; MEGAKARYOCYTE; NONHUMAN; SEQUENCE ANALYSIS;

ANIMALS; CELL DIFFERENTIATION; CELL PROLIFERATION; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, GEL; CHROMATOGRAPHY, HIGH PRESSURE LIQUID; ESCHERICHIA COLI; HUMAN GROWTH HORMONE; HUMANS; MASS SPECTROMETRY; MEGAKARYOCYTE PROGENITOR CELLS; MICE; PEPTIDES; RECOMBINANT FUSION PROTEINS; SEQUENCE ANALYSIS, PROTEIN;

ESCHERICHIA COLI; MURINAE;

EID: 84876678805     PISSN: 01757598     EISSN: 14320614     Source Type: Journal    
DOI: 10.1007/s00253-012-4553-7     Document Type: Article

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