High efficiency preparation of bioactive human α-defensin 6 in Escherichia coli Origami(DE3)pLysS by soluble fusion expression

Applied Microbiology and Biotechnology

Volumn 87, Issue 5, 2010, Pages 1935-1942

  Wang, Aiping ^a   Su, Yongping ^a   Wang, Song ^a   Shen, Mingqiang ^a   Chen, Fang ^a   Chen, Mo ^a   Ran, Xinze ^a   Cheng, Tianmin ^a   Wang, Junping ^a  

^a THIRD MILITARY MEDICAL UNIVERSITY   (China)

Author keywords

Antiviral activity; Escherichia coli; Expression; Human defensin 6; Purification

Indexed keywords

ANTIVIRAL ACTIVITIES; ANTIVIRAL ACTIVITY; CATION EXCHANGE CHROMATOGRAPHY; CATIONIC PEPTIDES; CHARACTERISTIC IDENTIFICATION; DEFENSINS; DIGESTIVE TRACT; EFFICIENT PRODUCTION; EPITHELIAL CELLS; EXPRESSION; EXPRESSION VECTORS; FRAGMENT ENCODING; FUSION EXPRESSION; FUSION PROTEINS; HERPES SIMPLEX VIRUS; HIGH EFFICIENCY; HIGH POTENCY; IN-VITRO; MUCOSAL IMMUNITY; N-TERMINAL AMINO ACID SEQUENCE; RECOGNITION SEQUENCE; RECOMBINANT PLASMID; THIOREDOXINS; WESTERN BLOTS;

AMINO ACIDS; CHROMATOGRAPHY; DIGESTIVE SYSTEM; ESCHERICHIA COLI; GEL PERMEATION CHROMATOGRAPHY; MASS SPECTROMETRY; ORGANIC ACIDS; PEPTIDES; PURIFICATION; VIRUSES;

GENE EXPRESSION;

ALPHA DEFENSIN; ALPHA DEFENSIN 6; ENTEROPEPTIDASE; HYBRID PROTEIN; NICKEL; SEPHAROSE; THIOREDOXIN; UNCLASSIFIED DRUG; ALPHA-DEFENSIN 6, HUMAN;

AMINO ACID; ANTIMICROBIAL ACTIVITY; BIOACTIVITY; CHROMATOGRAPHY; FECAL COLIFORM; GENE EXPRESSION; GENETIC ANALYSIS; GENETIC ENGINEERING; IMMUNITY; ION EXCHANGE; MASS SPECTROMETRY; MUCUS; OPTIMIZATION; PEPTIDE; PLASMID; POLYMERASE CHAIN REACTION; PROTEIN; RECOMBINATION; VIRAL DISEASE;

AMINO ACID SEQUENCE; AMINO TERMINAL SEQUENCE; ANTIVIRAL ACTIVITY; ARTICLE; CHROMATOGRAPHY; CONTROLLED STUDY; EPITHELIUM CELL; ESCHERICHIA COLI; EXPRESSION VECTOR; HERPES SIMPLEX VIRUS 2; HUMAN; HUMAN CELL; IN VITRO STUDY; MASS SPECTROMETRY; NONHUMAN; NUCLEOTIDE SEQUENCE; POLYACRYLAMIDE GEL ELECTROPHORESIS; PROTEIN ANALYSIS; PROTEIN EXPRESSION; PROTEIN FUNCTION; RECOMBINANT PLASMID; UTERINE CERVIX EPITHELIUM; WESTERN BLOTTING; AFFINITY CHROMATOGRAPHY; CELL LINE; DRUG EFFECTS; GENE EXPRESSION; GENE VECTOR; GENETIC RECOMBINATION; GENETICS; ION EXCHANGE CHROMATOGRAPHY; ISOLATION AND PURIFICATION; METABOLISM; MICROBIAL SENSITIVITY TEST; PLASMID; SEQUENCE ANALYSIS;

ALPHA-DEFENSINS; BLOTTING, WESTERN; CELL LINE; CHROMATOGRAPHY, AFFINITY; CHROMATOGRAPHY, ION EXCHANGE; ENTEROPEPTIDASE; ESCHERICHIA COLI; GENE EXPRESSION; GENETIC VECTORS; HERPESVIRUS 2, HUMAN; HUMANS; MASS SPECTROMETRY; MICROBIAL SENSITIVITY TESTS; PLASMIDS; RECOMBINANT FUSION PROTEINS; RECOMBINATION, GENETIC; SEQUENCE ANALYSIS;

ESCHERICHIA COLI; HUMAN HERPESVIRUS 2;

EID: 77955552227     PISSN: 01757598     EISSN: None     Source Type: Journal    
DOI: 10.1007/s00253-010-2688-y     Document Type: Article

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