Peptide agonist of the thrombopoietin receptor as potent as the natural cytokine

Science

Volumn 276, Issue 5319, 1997, Pages 1696-1699

  Cwirla, Steven E ^a   Balasubramanian, Palaniappan ^a   Duffin, David J ^a   Wagstrom, Christopher R ^a   Gates, Christian M ^a   Singer, Sara C ^b   Davis, Ann M ^a   Tansik, Robert L ^b   Mattheakis, Larry C ^a   Boytos, Chris M ^b   Schatz, Peter J ^a   Baccanari, David P ^b   Wrighton, Nicholas C ^a   Barrett, Ronald W ^a   Dower, William J ^a  

^a AFFYMAX RESEARCH INSTITUTE   (United States)

^b GLAXOSMITHKLINE   (United States)

Author keywords

[No Author keywords available]

Indexed keywords

CELL SURFACE RECEPTOR; CYTOKINE; INTERLEUKIN 3; PEPTIDE; THROMBOPOIETIN;

AMINO ACID SEQUENCE; ARTICLE; CARBOXY TERMINAL SEQUENCE; CONSENSUS SEQUENCE; DIMERIZATION; DISULFIDE BOND; ENZYME LINKED IMMUNOSORBENT ASSAY; PRIORITY JOURNAL; PROTEIN ANALYSIS; RECEPTOR BINDING; SEQUENCE ANALYSIS; THROMBOCYTE COUNT;

AMINO ACID SEQUENCE; ANIMALS; BINDING, COMPETITIVE; BLOOD PLATELETS; CELL DIVISION; CELL LINE; CELLS, CULTURED; CONSENSUS SEQUENCE; DIMERIZATION; ERYTHROPOIETIN; HEMATOPOIESIS; HUMANS; MEGAKARYOCYTES; MICE; MOLECULAR SEQUENCE DATA; NEOPLASM PROTEINS; OLIGOPEPTIDES; PEPTIDE LIBRARY; PLATELET COUNT; PROTO-ONCOGENE PROTEINS; RECEPTORS, CYTOKINE; RECEPTORS, THROMBOPOIETIN; RECOMBINANT PROTEINS; THROMBOPOIETIN; TRANSFECTION;

EID: 0030773876     PISSN: 00368075     EISSN: None     Source Type: Journal    
DOI: 10.1126/science.276.5319.1696     Document Type: Article

References (34)

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33. + cells with a StemSep lineage depletion kit (StemCell Technologies, Vancouver, BC).
34. We thank E. Tate, T. Dias, Y. Feng, E. Whitehom, and M. Bremer (receptor cloning and expression); R. Raab, G. Dawes, and C. Iverson (oligonucleotide synthesis and DNA sequencing); S. Podduturi and Q. Yin (peptide synthesis); S. Piplani, S. Johnson, and J. Dias (library screening); T. Cutler (proliferation assays) of the Affymax Research Institute; and A. Lee, I. Dev (human bone marrow assays), S. Rudolph, C. Merrell, and J. Dillberger (in vivo efficacy studies) of the Glaxo Wellcome Research Institute.